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AB250794

Anti-GCAP2 antibody [EPR14571] - BSA and Azide free

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Rabbit Recombinant Monoclonal GCAP2 antibody. Carrier free. Suitable for WB and reacts with Mouse, Rat samples.

View Alternative Names

GCAP2, GUCA1B, Guanylyl cyclase-activating protein 2, GCAP 2, Guanylate cyclase activator 1B

2 Images
Western blot - Anti-GCAP2 antibody [EPR14571] - BSA and Azide free (AB250794)
  • WB

Supplier Data

Western blot - Anti-GCAP2 antibody [EPR14571] - BSA and Azide free (AB250794)

This data was developed using ab184958, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-GCAP2 antibody [EPR14571] (<a href='/en-us/products/primary-antibodies/gcap2-antibody-epr14571-ab184958'>ab184958</a>) at 1/2000 dilution

All lanes:

Rat eyeball lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 23 kDa

false

Western blot - Anti-GCAP2 antibody [EPR14571] - BSA and Azide free (AB250794)
  • WB

Supplier Data

Western blot - Anti-GCAP2 antibody [EPR14571] - BSA and Azide free (AB250794)

This data was developed using ab184958, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-GCAP2 antibody [EPR14571] (<a href='/en-us/products/primary-antibodies/gcap2-antibody-epr14571-ab184958'>ab184958</a>) at 1/1000 dilution

All lanes:

Mouse eyeball lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 23 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14571

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab250794 is the carrier-free version of ab184958.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Guanylyl cyclase-activating protein 2 (GCAP2) also known as GUCA1B is a calcium-binding protein with a molecular weight of approximately 24 kDa. GCAP2 plays an important role in the visual system's phototransduction process. It is primarily expressed in the retina especially in photoreceptor cells. GCAP2 is part of a family of neuronal calcium sensor proteins that adjust their activities based on calcium concentration changes. These adjustments help regulate the sensitivity of photoreceptors to light.
Biological function summary

This protein modulates the activity of retinal guanylyl cyclases. In photoreceptors it forms complexes with these enzymes to regulate the synthesis of cyclic GMP in response to light. The availability of cyclic GMP is essential for the recovery phase of phototransduction which allows visual cells to respond to light changes efficiently. GCAP2 switching between active and inactive states adjusts the production of cyclic GMP depending on calcium levels inside the cells.

Pathways

GCAP2 features in the phototransduction cascade an essential pathway for converting light into visual signals. This cascade involves several proteins including rhodopsin transducin and phosphodiesterase. GCAP2’s role focuses on regulating guanylyl cyclases allowing the restoration of cyclic GMP levels which is important for returning ion channels to their open state after photon absorption. It is tightly connected to GCAP1 another guanylyl cyclase-activating protein with similar functions but they operate under slightly different calcium concentrations.

GCAP2 mutations have been linked to certain forms of cone and cone-rod dystrophies retinal degenerative disorders that affect vision. Changes in GCAP2 function influence cyclic GMP levels disrupting normal photoreceptor activity and survival. Mutations can interact with the rhodopsin promoter influencing disease progression and severity. Understanding GCAP2’s role provides potential targets for treating these retinal disorders and improving photoreceptor health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Stimulates two retinal guanylyl cyclases (GCs) GUCY2D and GUCY2F when free calcium ions concentration is low, and inhibits GUCY2D and GUCY2F when free calcium ions concentration is elevated (By similarity). This Ca(2+)-sensitive regulation of GCs is a key event in recovery of the dark state of rod photoreceptors following light exposure (By similarity). May be involved in cone photoreceptor response and recovery of response in bright light (By similarity).
See full target information GUCA1B
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com