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Rabbit Recombinant Monoclonal GCAT antibody. N-terminal. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

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Images

Western blot - Anti-GCAT antibody [EPR13450] - N-terminal (AB181094), expandable thumbnail
  • Western blot - Anti-GCAT antibody [EPR13450] - N-terminal (AB181094), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCAT antibody [EPR13450] - N-terminal (AB181094), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCAT antibody [EPR13450] - N-terminal (AB181094), expandable thumbnail
  • Immunoprecipitation - Anti-GCAT antibody [EPR13450] - N-terminal (AB181094), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IF
Human
Tested
Tested
Tested
Tested
Mouse
Predicted
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1/50 - 1/100
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/40
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50 - 1/100
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Pyridoxal phosphate (PLP) dependent enzyme, which catalyzes the cleavage of 2-amino-3-oxobutanoate to glycine and acetyl-CoA.

Alternative names

KBL, GCAT, AKB ligase, Aminoacetone synthase, Glycine acetyltransferase

Recommended products

Rabbit Recombinant Monoclonal GCAT antibody. N-terminal. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR13450
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

GCAT also known as glycine C-acetyltransferase functions as a mitochondrial enzyme involved in glycine metabolism. It has a molecular mass of approximately 44 kDa. Researchers often find GCAT expressed in the liver where it plays a significant role in metabolic processes related to amino acid synthesis and catabolism. Being predominantly cytosolic GCAT contributes to the detoxification pathways by participating in the conversion of glycine to other metabolites.

Biological function summary

In glycine cleavage system GCAT operates as part of a complex essential for the regulation of amino acid levels. The enzyme accepts substrates from reactions facilitated by other components such as T-protein and P-protein and is vital in maintaining the balance of glycine and other amino acids within the cell. Dysfunction in any part of this system can result in elevated levels of toxic metabolic intermediates leading to cellular stress and potential tissue damage.

Pathways

GCAT is an integral element in the mitochondrial glycine cleavage system linking it to broader metabolic pathways such as the Krebs cycle and oxidative phosphorylation. This position between pathways underlines its connection with proteins such as glycine dehydrogenase and aminomethyltransferase. This interconnectedness suggests that imbalances involving GCAT can disrupt metabolic homeostasis influencing energy production at the cellular level.

Associated diseases and disorders

Defects or dysregulation in GCAT activity link to various metabolic conditions including nonketotic hyperglycinemia and liver diseases. Patients with nonketotic hyperglycinemia show accumulation of glycine leading to neurological issues conditions typically associated with deficiencies in glycine metabolism enzymes like P-protein. In the context of liver diseases alterations in GCAT's function affect the liver's ability to process nitrogen highlighting its importance in liver homeostasis and function.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-GCAT antibody [EPR13450] - N-terminal (ab181094), expandable thumbnail

    Western blot - Anti-GCAT antibody [EPR13450] - N-terminal (ab181094)

    All lanes: Western blot - Anti-GCAT antibody [EPR13450] - N-terminal (ab181094) at 1/5000 dilution

    Lane 1: BxPC-3 cell lysate at 20 µg

    Lane 2: HepG2 cell lysate at 20 µg

    Lane 3: U87-MG cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 45 kDa

    Observed band size: 45 kDa

  • Western blot - Anti-GCAT antibody [EPR13450] - N-terminal (ab181094), expandable thumbnail

    Western blot - Anti-GCAT antibody [EPR13450] - N-terminal (ab181094)

    All lanes: Western blot - Anti-GCAT antibody [EPR13450] - N-terminal (ab181094) at 1/5000 dilution

    All lanes: Human fetal liver tissue lysate

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab136636) at 1/500 dilution

    Predicted band size: 45 kDa

    Observed band size: 45 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCAT antibody [EPR13450] - N-terminal (ab181094), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCAT antibody [EPR13450] - N-terminal (ab181094)

    Immunohistochemical analysis of Human heart tissue, staining GCAT with ab181094 at 1/100 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCAT antibody [EPR13450] - N-terminal (ab181094), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCAT antibody [EPR13450] - N-terminal (ab181094)

    Immunohistochemical analysis of Human pancreas tissue, staining GCAT with ab181094 at 1/100 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-GCAT antibody [EPR13450] - N-terminal (ab181094), expandable thumbnail

    Immunoprecipitation - Anti-GCAT antibody [EPR13450] - N-terminal (ab181094)

    Western blot analysis on immunoprecipitation pellet from HepG2 cell lysate (lane 1) or negative control (lane 2), labeling GCAT immunoprecipitated using ab181094 at 1/40 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    All lanes: Immunoprecipitation - Anti-GCAT antibody [EPR13450] - N-terminal (ab181094)

    Predicted band size: 45 kDa

    Observed band size: 45 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-GCAT antibody [EPR13450] - N-terminal (ab181094), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-GCAT antibody [EPR13450] - N-terminal (ab181094)

    Immunofluorescence analysis of HepG2 cells, staining GCAT (green) with ab181094 at 1/100 dilution. An Alexa Fluor®488-conjugated goat anti-rabbit IgG was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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