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AB128173

Anti-GCC185 antibody

4

(5 Reviews)

|

(2 Publications)

Rabbit Polyclonal GCC185 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human GCC2 aa 1-50.

View Alternative Names

KIAA0336, RANBP2L4, GCC2, GRIP and coiled-coil domain-containing protein 2, 185 kDa Golgi coiled-coil protein, CLL-associated antigen KW-11, CTCL tumor antigen se1-1, Ran-binding protein 2-like 4, Renal carcinoma antigen NY-REN-53, GCC185, RanBP2L4

2 Images
Immunoprecipitation - Anti-GCC185 antibody (AB128173)
  • IP

Unknown

Immunoprecipitation - Anti-GCC185 antibody (AB128173)

Detection of GCC185 by Western Blot of Immunprecipitate.

ab128173, at 1 μg/ml, staining GCC185 in HeLa whole cell lysate immunoprecipitated using ab128173 at 6 μg/mg lysate (1 mg/IP; 20% of IP loaded/lane).

Detection : Chemiluminescence with exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-GCC185 antibody (ab128173)

Predicted band size: 196 kDa

false

Western blot - Anti-GCC185 antibody (AB128173)
  • WB

Unknown

Western blot - Anti-GCC185 antibody (AB128173)

All lanes:

Western blot - Anti-GCC185 antibody (ab128173) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

293T whole cell lysate at 50 µg

Lane 4:

Jurkat whole cell lysate at 50 µg

Predicted band size: 196 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human GCC2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q8IWJ2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab128173 is affinity purified using an epitope specific to GCC185 immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GCC185 also known as Golgi coiled-coil protein 185 plays a mechanical role in the tethering of Golgi membranes. It has a mass of approximately 185 kDa and localizes to the trans-Golgi network (TGN). GCC185 is responsible for maintaining Golgi structure and integrity by interacting with various membrane vesicles and microtubules. It is broadly expressed in different cell types emphasizing its general importance in maintaining the secretory pathway across various tissues.
Biological function summary

GCC185 facilitates vesicle tethering at the Golgi apparatus. It is a component of the larger Golgin family which is a group of coiled-coil proteins. These proteins assist in modulating Golgi organization and function by interactively recruiting and tethering vesicles from the trans-Golgi to other cellular destinations. GCC185 specifically binds to the Rab GTPases contributing to membrane trafficking and ensuring the effective transport of proteins and lipids within the cell.

Pathways

The function of GCC185 integrates within the intracellular trafficking and sorting pathways. It notably participates in the trafficking of lysosomal hydrolases through the mannose-6-phosphate (M6P) receptor pathway. GCC185 interacts with other Golgin family members and proteins like Rab9 facilitating retrograde transport from endosomes to the Golgi an essential process for protein sorting and recycling.

Improper functioning of GCC185 is linked to disorders involving lysosomal storage and Golgi dysfunction. One significant condition associated with GCC185 is neurodegenerative disease where defects in protein sorting and trafficking can impair neuronal function. Moreover disruptions in the GCC185-Rab9 connection are implicated in the pathology of disorders like Alzheimer's disease as they contribute to the mistrafficking of amyloid precursor proteins further illustrating the protein's critical role in maintaining cellular homeostasis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Golgin which probably tethers transport vesicles to the trans-Golgi network (TGN) and regulates vesicular transport between the endosomes and the Golgi. As a RAB9A effector it is involved in recycling of the mannose 6-phosphate receptor from the late endosomes to the TGN. May also play a role in transport between the recycling endosomes and the Golgi. Required for maintenance of the Golgi structure, it is involved in the biogenesis of noncentrosomal, Golgi-associated microtubules through recruitment of CLASP1 and CLASP2.
See full target information GCC2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 7:426 PubMed38589567

2024

Targeting TRIP13 in favorable histology Wilms tumor with nuclear export inhibitors synergizes with doxorubicin.

Applications

Unspecified application

Species

Unspecified reactive species

Karuna Mittal,Garrett W Cooper,Benjamin P Lee,Yongdong Su,Katie T Skinner,Jenny Shim,Hunter C Jonus,Won Jun Kim,Mihir Doshi,Diego Almanza,Bryan D Kynnap,Amanda L Christie,Xiaoping Yang,Glenn S Cowley,Brittaney A Leeper,Christopher L Morton,Bhakti Dwivedi,Taylor Lawrence,Manali Rupji,Paula Keskula,Stephanie Meyer,Catherine M Clinton,Manoj Bhasin,Brian D Crompton,Yuen-Yi Tseng,Jesse S Boehm,Keith L Ligon,David E Root,Andrew J Murphy,David M Weinstock,Prafulla C Gokhale,Jennifer M Spangle,Miguel N Rivera,Elizabeth A Mullen,Kimberly Stegmaier,Kelly C Goldsmith,William C Hahn,Andrew L Hong

Life sciences 229:13-20 PubMed30953643

2019

HIV-1 Nef-GCC185 interaction regulates assembly of cellular protein complexes at TGN targeting MHC-I downregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Sushila Kumari,Manjeet Kumar,Richa Verma,Jimut Kanti Ghosh,Raj Kamal Tripathi
View all publications
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Product promise

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