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Rabbit Recombinant Monoclonal GCDFP 15 antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 6 publications.


Images

Western blot - Anti-GCDFP 15 antibody [EP1582Y] (AB62363), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [EP1582Y] (AB62363), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-GCDFP 15 antibody [EP1582Y] (AB62363), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-GCDFP 15 antibody [EP1582Y] (AB62363), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [EP1582Y] (AB62363), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-PICC/IFFlow Cyt (Intra)
Human
Not recommended
Tested
Tested
Tested
Tested

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/2000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50 - 1/100
Notes

-

Tested
Tested

Species
Human
Dilution info
1/20
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Associated Products

Select an associated product type

5 products for Alternative Product

Target data

Alternative names

GCDFP15, GPIP4, PIP, Prolactin-inducible protein, Gross cystic disease fluid protein 15, Prolactin-induced protein, Secretory actin-binding protein, gp17, GCDFP-15, SABP

Recommended products

Rabbit Recombinant Monoclonal GCDFP 15 antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 6 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EP1582Y
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

GCDFP-15 also known as gross cystic disease fluid protein 15 is a glycoprotein with a molecular weight of approximately 15 kDa. It is commonly expressed in apocrine glands particularly in breast tissue. Immunohistochemical studies show that GCDFP-15 is a marker for breast apocrine metaplasia and is frequently used in identifying apocrine carcinoma. The presence of GCDFP-15 in other sites like salivary glands suggests its broader role within glandular tissues.

Biological function summary

GCDFP-15 functions as a secretory protein involved in the regulation of fluid composition in ducts. It is not known to be part of a larger complex but acts independently. This protein plays a role in stabilizing the microenvironment of ductal secretions which assists in the preservation of ductal lining and the maintenance of tissue integrity. The modulation of fluid content by GCDFP-15 is essential in maintaining the function of glandular tissues.

Pathways

GCDFP-15 is integral to lipid metabolism pathways and may indirectly influence signal transduction. It shows interaction with other proteins like prolactin and fatty acid-binding protein suggesting a broader role in lipid-related biological processes. The involvement of GCDFP-15 in these pathways indicates its contribution to the regulation of metabolic homeostasis within glandular tissues.

Associated diseases and disorders

GCDFP-15 has notable significance in breast cancer particularly in differentiating apocrine-type carcinoma from other subtypes. The expression of GCDFP-15 is considered a biomarker in breast pathology for diagnostic purposes. In addition its role in salivary gland disorders is under investigation given its expression in the ducts of salivary glands. The protein's relationship with markers like mammaglobin further highlights its clinical relevance in distinguishing between glandular-related pathologies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Western blot - Anti-GCDFP 15 antibody [EP1582Y] (ab62363), expandable thumbnail

    Western blot - Anti-GCDFP 15 antibody [EP1582Y] (ab62363)

    All lanes: Western blot - Anti-GCDFP 15 antibody [EP1582Y] (ab62363) at 1/2000 dilution

    All lanes: T47D cell lysate at 10 µg

    Secondary

    All lanes: goat anti rabbit HRP at 1/2000 dilution

    Predicted band size: 17 kDa

    Observed band size: 17 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [EP1582Y] (ab62363), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [EP1582Y] (ab62363)

    Immunohistochemical analysis of GCDFP 15 expression in paraffin embedded human breast carcinoma using 1/100-1/250 dilution of ab62363

  • Immunocytochemistry/ Immunofluorescence - Anti-GCDFP 15 antibody [EP1582Y] (ab62363), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-GCDFP 15 antibody [EP1582Y] (ab62363)

    Immunocytochemistry/Immunofluorescence analysis of MCF-7 (human breast carcinoma) cells labelling GCDFP 15 with ab62363 at 1/100. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton-X. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counter-stained with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 Anti-Tubulin antibody (1/1000) followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 AlexaFluor®594 Goat anti-Mouse secondary (1/1000). Nuclei were counterstained with DAPI (blue).

    For negative control 1, ab62363 at dilution of 1/100 was used followed by anti-mouse secondary antibody (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/1000 dilution. For negative control 2, Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291; 1/1000 was used followed by anti-rabbit secondary antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.

    Confocal image showing cytoplasmic staining on MCF7 cells.

  • Flow Cytometry (Intracellular) - Anti-GCDFP 15 antibody [EP1582Y] (ab62363), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-GCDFP 15 antibody [EP1582Y] (ab62363)

    Intracellular Flow Cytometry analysis of T47D (human mammary gland ductal carcinoma) (pink) with ab62363 at dilution of 1/20. The secondary antibody used was goat anti rabbit IgG (FITC) at dilution of 1/150. Cells were fixed with 2% paraformaldehyde. Isotype control antibody used was Rabbit monoclonal IgG (green).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [EP1582Y] (ab62363), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [EP1582Y] (ab62363)

    ab62363 showing positive staining in Salivary gland tissue.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [EP1582Y] (ab62363), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [EP1582Y] (ab62363)

    ab62363 showing negative staining in Hepatocellular carcinoma tissue.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [EP1582Y] (ab62363), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [EP1582Y] (ab62363)

    ab62363 showing negative staining in Prostate hyperplasia tissue.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [EP1582Y] (ab62363), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [EP1582Y] (ab62363)

    ab62363 showing positive staining in Cystic breast disease tissue.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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