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AB62363

Anti-GCDFP 15 antibody [EP1582Y]

1

(1 Review)

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(6 Publications)

Rabbit Recombinant Monoclonal GCDFP 15 antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 6 publications.

View Alternative Names

GCDFP15, GPIP4, PIP, Prolactin-inducible protein, Gross cystic disease fluid protein 15, Prolactin-induced protein, Secretory actin-binding protein, gp17, GCDFP-15, SABP

8 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [EP1582Y] (AB62363)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [EP1582Y] (AB62363)

ab62363 showing positive staining in Salivary gland tissue.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [EP1582Y] (AB62363)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [EP1582Y] (AB62363)

ab62363 showing positive staining in Cystic breast disease tissue.

Immunocytochemistry/ Immunofluorescence - Anti-GCDFP 15 antibody [EP1582Y] (AB62363)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GCDFP 15 antibody [EP1582Y] (AB62363)

Immunocytochemistry/Immunofluorescence analysis of MCF-7 (human breast carcinoma) cells labelling GCDFP 15 with ab62363 at 1/100. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton-X. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counter-stained with ab7291 Anti-Tubulin antibody (1/1000) followed by ab150120 AlexaFluor®594 Goat anti-Mouse secondary (1/1000). Nuclei were counterstained with DAPI (blue).

For negative control 1, ab62363 at dilution of 1/100 was used followed by anti-mouse secondary antibody (ab150120) at 1/1000 dilution. For negative control 2, ab7291; 1/1000 was used followed by anti-rabbit secondary antibody (ab150077) at 1/1000 dilution.

Confocal image showing cytoplasmic staining on MCF7 cells.

Flow Cytometry (Intracellular) - Anti-GCDFP 15 antibody [EP1582Y] (AB62363)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-GCDFP 15 antibody [EP1582Y] (AB62363)

Intracellular Flow Cytometry analysis of T47D (human mammary gland ductal carcinoma) (pink) with ab62363 at dilution of 1/20. The secondary antibody used was goat anti rabbit IgG (FITC) at dilution of 1/150. Cells were fixed with 2% paraformaldehyde. Isotype control antibody used was Rabbit monoclonal IgG (green).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [EP1582Y] (AB62363)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [EP1582Y] (AB62363)

ab62363 showing negative staining in Hepatocellular carcinoma tissue.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [EP1582Y] (AB62363)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [EP1582Y] (AB62363)

Immunohistochemical analysis of GCDFP 15 expression in paraffin embedded human breast carcinoma using 1/100-1/250 dilution of ab62363

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [EP1582Y] (AB62363)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [EP1582Y] (AB62363)

ab62363 showing negative staining in Prostate hyperplasia tissue.

Western blot - Anti-GCDFP 15 antibody [EP1582Y] (AB62363)
  • WB

Unknown

Western blot - Anti-GCDFP 15 antibody [EP1582Y] (AB62363)

All lanes:

Western blot - Anti-GCDFP 15 antibody [EP1582Y] (ab62363) at 1/2000 dilution

All lanes:

T47D cell lysate at 10 µg

Secondary

All lanes:

goat anti rabbit HRP at 1/2000 dilution

Predicted band size: 17 kDa

Observed band size: 17 kDa

false

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-GCDFP 15 antibody [EP1582Y]

  • Carrier free

    Anti-GCDFP 15 antibody [EP1582Y] - BSA and Azide free

  • 578 PE

    PE Anti-GCDFP 15 antibody [EP1582Y]

  • 660 APC

    APC Anti-GCDFP 15 antibody [EP1582Y]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-GCDFP 15 antibody [EP1582Y]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-GCDFP 15 antibody [EP1582Y]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-GCDFP 15 antibody [EP1582Y]

  • HRP

    HRP Anti-GCDFP 15 antibody [EP1582Y]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-GCDFP 15 antibody [EP1582Y]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-GCDFP 15 antibody [EP1582Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1582Y

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), WB, IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

Primary Antibodies

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  • 0
  • 0
Primary Antibodies

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Primary Antibodies

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  • 0
  • 0
Primary Antibodies

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [SP185] - C-terminal (AB183321)

  • 0
  • 0
Primary Antibodies

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [SP185] - BSA and Azide free (AB238776)

  • 0
  • 0

Complementary Products

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  • 0
  • 0
Primary Antibodies

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Primary Antibodies

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPSIN A antibody [EPR6252] (AB133249)

  • 0
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Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

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  • 5
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Primary Antibodies

AB133273

Anti-Androgen Receptor antibody [EPR1535(2)]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (AB133273)

  • 5
  • 11
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/20", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GCDFP-15 also known as gross cystic disease fluid protein 15 is a glycoprotein with a molecular weight of approximately 15 kDa. It is commonly expressed in apocrine glands particularly in breast tissue. Immunohistochemical studies show that GCDFP-15 is a marker for breast apocrine metaplasia and is frequently used in identifying apocrine carcinoma. The presence of GCDFP-15 in other sites like salivary glands suggests its broader role within glandular tissues.
Biological function summary

GCDFP-15 functions as a secretory protein involved in the regulation of fluid composition in ducts. It is not known to be part of a larger complex but acts independently. This protein plays a role in stabilizing the microenvironment of ductal secretions which assists in the preservation of ductal lining and the maintenance of tissue integrity. The modulation of fluid content by GCDFP-15 is essential in maintaining the function of glandular tissues.

Pathways

GCDFP-15 is integral to lipid metabolism pathways and may indirectly influence signal transduction. It shows interaction with other proteins like prolactin and fatty acid-binding protein suggesting a broader role in lipid-related biological processes. The involvement of GCDFP-15 in these pathways indicates its contribution to the regulation of metabolic homeostasis within glandular tissues.

GCDFP-15 has notable significance in breast cancer particularly in differentiating apocrine-type carcinoma from other subtypes. The expression of GCDFP-15 is considered a biomarker in breast pathology for diagnostic purposes. In addition its role in salivary gland disorders is under investigation given its expression in the ducts of salivary glands. The protein's relationship with markers like mammaglobin further highlights its clinical relevance in distinguishing between glandular-related pathologies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information PIP
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

iScience 25:103592 PubMed35005541

2022

Salivary ZG16B expression loss follows exocrine gland dysfunction related to oral chronic graft-versus-host disease.

Applications

Unspecified application

Species

Unspecified reactive species

Ana Caroline Costa-da-Silva,Marit H Aure,Joshua Dodge,Daniel Martin,Susan Dhamala,Monica Cho,Jeremy J Rose,Carol W Bassim,Kiran Ambatipudi,Frances T Hakim,Steven Z Pavletic,Jacqueline W Mays

Breast cancer research : BCR 23:102 PubMed34736512

2021

Secreted indicators of androgen receptor activity in breast cancer pre-clinical models.

Applications

Unspecified application

Species

Unspecified reactive species

Toru Hanamura,Jessica L Christenson,Kathleen I O'Neill,Emmanuel Rosas,Nicole S Spoelstra,Michelle M Williams,Jennifer K Richer

Journal of translational medicine 13:371 PubMed26608305

2015

Differential urinary glycoproteome analysis of type 2 diabetic nephropathy using 2D-LC-MS/MS and iTRAQ quantification.

Applications

WB

Species

Unspecified reactive species

Zhengguang Guo,Xuejiao Liu,Menglin Li,Chen Shao,Jianling Tao,Wei Sun,Mingxi Li

Journal of genetic syndromes & gene therapy 4: PubMed24416635

2014

Gross Cystic Disease Fluid Protein-15(GCDFP-15)/Prolactin-Inducible Protein (PIP) as Functional Salivary Biomarker for Primary Sjögren's Syndrome.

Applications

IHC

Species

Unspecified reactive species

A Gallo,D Martini,F Sernissi,C Giacomelli,P Pepe,C Rossi,Pp Riveros,M Mosca,I Alevizos,C Baldini

Breast cancer research : BCR 14:R111 PubMed22817771

2012

Prolactin-induced protein mediates cell invasion and regulates integrin signaling in estrogen receptor-negative breast cancer.

Applications

WB, IHC-P

Species

Human, Human

Ali Naderi,Michelle Meyer

Molecular cancer 9:258 PubMed20863401

2010

Runx2 transcriptome of prostate cancer cells: insights into invasiveness and bone metastasis.

Applications

WB

Species

Human

Sanjeev K Baniwal,Omar Khalid,Yankel Gabet,Ruchir R Shah,Daniel J Purcell,Deepak Mav,Alice E Kohn-Gabet,Yunfan Shi,Gerhard A Coetzee,Baruch Frenkel
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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