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AB235998

Anti-GCDFP 15 antibody [EP1582Y] - BSA and Azide free

1

(1 Review)

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(1 Publication)

Rabbit Recombinant Monoclonal GCDFP 15 antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

GCDFP15, GPIP4, PIP, Prolactin-inducible protein, Gross cystic disease fluid protein 15, Prolactin-induced protein, Secretory actin-binding protein, gp17, GCDFP-15, SABP

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [EP1582Y] - BSA and Azide free (AB235998)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [EP1582Y] - BSA and Azide free (AB235998)

ab62363 showing positive staining in Salivary gland tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab62363).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [EP1582Y] - BSA and Azide free (AB235998)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [EP1582Y] - BSA and Azide free (AB235998)

ab62363 showing positive staining in Cystic breast disease tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab62363).

Immunocytochemistry/ Immunofluorescence - Anti-GCDFP 15 antibody [EP1582Y] - BSA and Azide free (AB235998)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GCDFP 15 antibody [EP1582Y] - BSA and Azide free (AB235998)

Immunocytochemistry/Immunofluorescence analysis of MCF-7 (human breast carcinoma) cells labelling GCDFP 15 with ab62363 at 1/100. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton-X. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counter-stained with ab7291 Anti-Tubulin antibody (1/1000) followed by ab150120   AlexaFluor®594 Goat anti-Mouse secondary (1/1000). Nuclei were counterstained with DAPI (blue).

For negative control 1, ab62363 at dilution of 1/100 was used followed by anti-mouse secondary antibody (ab150120) at 1/1000 dilution. For negative control 2, ab7291; 1/1000 was used followed by anti-rabbit secondary antibody (ab150077) at 1/1000 dilution.

Confocal image showing cytoplasmic staining on MCF7 cells.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab62363).

Flow Cytometry (Intracellular) - Anti-GCDFP 15 antibody [EP1582Y] - BSA and Azide free (AB235998)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-GCDFP 15 antibody [EP1582Y] - BSA and Azide free (AB235998)

Intracellular Flow Cytometry analysis of T47D (human mammary gland ductal carcinoma) (pink) with ab62363 at dilution of 1/20. The secondary antibody used was goat anti rabbit IgG (FITC) at dilution of 1/150. Cells were fixed with 2% paraformaldehyde. Isotype control antibody used was Rabbit monoclonal IgG (green).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab62363).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [EP1582Y] - BSA and Azide free (AB235998)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [EP1582Y] - BSA and Azide free (AB235998)

ab62363 showing negative staining in Hepatocellular carcinoma tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab62363).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [EP1582Y] - BSA and Azide free (AB235998)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [EP1582Y] - BSA and Azide free (AB235998)

Immunohistochemical analysis of GCDFP 15 expression in paraffin embedded human breast carcinoma using 1/100-1/250 dilution of ab62363This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab62363).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [EP1582Y] - BSA and Azide free (AB235998)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [EP1582Y] - BSA and Azide free (AB235998)

ab62363 showing negative staining in Prostate hyperplasia tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab62363).

  • Unconjugated

    Anti-GCDFP 15 antibody [EP1582Y]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-GCDFP 15 antibody [EP1582Y]

  • 578 PE

    PE Anti-GCDFP 15 antibody [EP1582Y]

  • 660 APC

    APC Anti-GCDFP 15 antibody [EP1582Y]

  • HRP

    HRP Anti-GCDFP 15 antibody [EP1582Y]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-GCDFP 15 antibody [EP1582Y]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-GCDFP 15 antibody [EP1582Y]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-GCDFP 15 antibody [EP1582Y]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-GCDFP 15 antibody [EP1582Y]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-GCDFP 15 antibody [EP1582Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1582Y

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, Flow Cyt (Intra), IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }
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Product details

ab235998 is the carrier-free version of ab62363.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GCDFP-15 also known as gross cystic disease fluid protein 15 is a glycoprotein with a molecular weight of approximately 15 kDa. It is commonly expressed in apocrine glands particularly in breast tissue. Immunohistochemical studies show that GCDFP-15 is a marker for breast apocrine metaplasia and is frequently used in identifying apocrine carcinoma. The presence of GCDFP-15 in other sites like salivary glands suggests its broader role within glandular tissues.
Biological function summary

GCDFP-15 functions as a secretory protein involved in the regulation of fluid composition in ducts. It is not known to be part of a larger complex but acts independently. This protein plays a role in stabilizing the microenvironment of ductal secretions which assists in the preservation of ductal lining and the maintenance of tissue integrity. The modulation of fluid content by GCDFP-15 is essential in maintaining the function of glandular tissues.

Pathways

GCDFP-15 is integral to lipid metabolism pathways and may indirectly influence signal transduction. It shows interaction with other proteins like prolactin and fatty acid-binding protein suggesting a broader role in lipid-related biological processes. The involvement of GCDFP-15 in these pathways indicates its contribution to the regulation of metabolic homeostasis within glandular tissues.

GCDFP-15 has notable significance in breast cancer particularly in differentiating apocrine-type carcinoma from other subtypes. The expression of GCDFP-15 is considered a biomarker in breast pathology for diagnostic purposes. In addition its role in salivary gland disorders is under investigation given its expression in the ducts of salivary glands. The protein's relationship with markers like mammaglobin further highlights its clinical relevance in distinguishing between glandular-related pathologies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information PIP
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in aging neuroscience 12:575481 PubMed33328957

2020

Alpha-Synuclein Accumulation and Its Phosphorylation in the Enteric Nervous System of Patients Without Neurodegeneration: An Explorative Study.

Applications

Unspecified application

Species

Unspecified reactive species

Lu-Lu Bu,Kai-Xun Huang,De-Zhi Zheng,Dan-Yu Lin,Ying Chen,Xiu-Na Jing,Yan-Ran Liang,En-Xiang Tao
View all publications
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Product promise

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