Rabbit Recombinant Monoclonal GCDFP 15 antibody. C-terminal. Suitable for WB, IHC-P and reacts with Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [SP185] - C-terminal (AB183321), expandable thumbnail](https://content.abcam.com/products/images/gcdfp-15-antibody-sp185-c-terminal-ab183321--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img39645.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [SP185] - C-terminal (AB183321)")
![Western blot - Anti-GCDFP 15 antibody [SP185] - C-terminal (AB183321), expandable thumbnail](https://content.abcam.com/products/images/gcdfp-15-antibody-sp185-c-terminal-ab183321--western-blot-img88535.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GCDFP 15 antibody [SP185] - C-terminal (AB183321)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [SP185] - C-terminal (AB183321), expandable thumbnail](https://content.abcam.com/products/images/gcdfp-15-antibody-sp185-c-terminal-ab183321--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img138716.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [SP185] - C-terminal (AB183321)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [SP185] - C-terminal (AB183321), expandable thumbnail](https://content.abcam.com/products/images/gcdfp-15-antibody-sp185-c-terminal-ab183321--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img132220.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [SP185] - C-terminal (AB183321)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [SP185] - C-terminal (AB183321), expandable thumbnail](https://content.abcam.com/products/images/gcdfp-15-antibody-sp185-c-terminal-ab183321--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img109421.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [SP185] - C-terminal (AB183321)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/400 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Alternative names
GCDFP15, GPIP4, PIP, Prolactin-inducible protein, Gross cystic disease fluid protein 15, Prolactin-induced protein, Secretory actin-binding protein, gp17, GCDFP-15, SABP
Recommended products
Rabbit Recombinant Monoclonal GCDFP 15 antibody. C-terminal. Suitable for WB, IHC-P and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- SP185
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Cow: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GCDFP-15 also known as gross cystic disease fluid protein 15 is a glycoprotein with a molecular weight of approximately 15 kDa. It is commonly expressed in apocrine glands particularly in breast tissue. Immunohistochemical studies show that GCDFP-15 is a marker for breast apocrine metaplasia and is frequently used in identifying apocrine carcinoma. The presence of GCDFP-15 in other sites like salivary glands suggests its broader role within glandular tissues.
- Biological function summary
GCDFP-15 functions as a secretory protein involved in the regulation of fluid composition in ducts. It is not known to be part of a larger complex but acts independently. This protein plays a role in stabilizing the microenvironment of ductal secretions which assists in the preservation of ductal lining and the maintenance of tissue integrity. The modulation of fluid content by GCDFP-15 is essential in maintaining the function of glandular tissues.
- Pathways
GCDFP-15 is integral to lipid metabolism pathways and may indirectly influence signal transduction. It shows interaction with other proteins like prolactin and fatty acid-binding protein suggesting a broader role in lipid-related biological processes. The involvement of GCDFP-15 in these pathways indicates its contribution to the regulation of metabolic homeostasis within glandular tissues.
- Associated diseases and disorders
GCDFP-15 has notable significance in breast cancer particularly in differentiating apocrine-type carcinoma from other subtypes. The expression of GCDFP-15 is considered a biomarker in breast pathology for diagnostic purposes. In addition its role in salivary gland disorders is under investigation given its expression in the ducts of salivary glands. The protein's relationship with markers like mammaglobin further highlights its clinical relevance in distinguishing between glandular-related pathologies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [SP185] - C-terminal (ab183321), expandable thumbnail](https://content.abcam.com/products/images/gcdfp-15-antibody-sp185-c-terminal-ab183321--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img39645.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [SP185] - C-terminal (ab183321)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [SP185] - C-terminal (ab183321)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast carcinoma tissue sections labeling GCDFP 15 with ab183321 at 1/100 dilution (2.21 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human breast carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab183321 for 10 mins at room temperature.![Western blot - Anti-GCDFP 15 antibody [SP185] - C-terminal (ab183321), expandable thumbnail](https://content.abcam.com/products/images/gcdfp-15-antibody-sp185-c-terminal-ab183321--western-blot-img88535.jpg "Western blot - Anti-GCDFP 15 antibody [SP185] - C-terminal (ab183321)")
Western blot - Anti-GCDFP 15 antibody [SP185] - C-terminal (ab183321)
All lanes: Western blot - Anti-GCDFP 15 antibody [SP185] - C-terminal (ab183321) at 1/400 dilution
All lanes: T47D cell lysate
Predicted band size: 17 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [SP185] - C-terminal (ab183321), expandable thumbnail](https://content.abcam.com/products/images/gcdfp-15-antibody-sp185-c-terminal-ab183321--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img138716.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [SP185] - C-terminal (ab183321)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [SP185] - C-terminal (ab183321)
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling GCDFP with ab183321 at 1/100 dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [SP185] - C-terminal (ab183321), expandable thumbnail](https://content.abcam.com/products/images/gcdfp-15-antibody-sp185-c-terminal-ab183321--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img132220.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [SP185] - C-terminal (ab183321)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [SP185] - C-terminal (ab183321)
Immunohistochemical analysis of paraffin-embedded Human breast ductal carcinoma tissue labeling GCDFP with ab183321 at 1/100 dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [SP185] - C-terminal (ab183321), expandable thumbnail](https://content.abcam.com/products/images/gcdfp-15-antibody-sp185-c-terminal-ab183321--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img109421.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [SP185] - C-terminal (ab183321)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [SP185] - C-terminal (ab183321)
Immunohistochemical analysis of paraffin-embedded Human breast ductal carcinoma tissue labeling GCDFP with ab183321 at 1/100 dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [SP185] - C-terminal (ab183321), expandable thumbnail](https://content.abcam.com/products/images/gcdfp-15-antibody-sp185-c-terminal-ab183321--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img107813.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [SP185] - C-terminal (ab183321)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [SP185] - C-terminal (ab183321)
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling GCDFP with ab183321 at 1/100 dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [SP185] - C-terminal (ab183321), expandable thumbnail](https://content.abcam.com/products/images/gcdfp-15-antibody-sp185-c-terminal-ab183321--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img107813.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [SP185] - C-terminal (ab183321)")
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