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AB112998

Anti-GCDH/GCD antibody [3E9BA3BF5]

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(1 Publication)

Mouse Monoclonal GCDH/GCD antibody. Suitable for Flow Cyt, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

GCD, GCDH

2 Images
Flow Cytometry - Anti-GCDH/GCD antibody [3E9BA3BF5] (AB112998)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-GCDH/GCD antibody [3E9BA3BF5] (AB112998)

ab112998 at 1ug/ml staining GCDH/GCD in HeLa cells by Flow Cytometry (blue). Isotype control antibody (red).

Immunocytochemistry/ Immunofluorescence - Anti-GCDH/GCD antibody [3E9BA3BF5] (AB112998)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GCDH/GCD antibody [3E9BA3BF5] (AB112998)

ab112998 at 1 μg/ml staining GCDH/GCD in fibroblast cells by immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red). Nuclei were stained with DAPI.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3E9BA3BF5

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

Flow Cyt, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

Primary Antibodies

AB182152

Anti-GCDH/GCD antibody [EPR14864(B)]

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Flow Cytometry (Intracellular) - Anti-GCDH/GCD antibody [EPR14864(B)] (AB182152)

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Primary Antibodies

AB232774

Anti-GCDH/GCD antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDH/GCD antibody (AB232774)

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Complementary Products

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Proteins & Peptides

AB98118

Recombinant Human GCDH/GCD protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human GCDH/GCD protein (His tag N-Terminus) (AB98118)

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Primary Antibodies

AB213511

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Flow Cytometry - Anti-mCherry antibody [EPR20579] (AB213511)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg/mL", "FlowCyt-species-notes": "<p>0.1% Triton X-100 permeabilization recommended.</p><p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
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Properties and storage information

Form
Liquid
Purification technique
Proprietary technique
Purification notes
ab112998 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Monoclonal purity was near homogeneity as judged by SDS-PAGE (>95%).
Storage buffer
pH: 7.5 Preservative: 0.02% Sodium azide Constituents: 99.98% HEPES buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GCDH also known as glutaryl-CoA dehydrogenase is an enzyme involved in the catabolic breakdown of specific amino acids. This protein harbors a mass of approximately 45 kDa and functions primarily within the mitochondria. GCDH expression occurs in various tissues including the liver kidney and brain. The enzyme plays a mechanical role by catalyzing the decarboxylation of glutaryl-CoA to crotonyl-CoA facilitating the removal of carbon dioxide in this biochemical process.
Biological function summary

This enzyme acts as an important component in the lysine hydroxylysine and tryptophan degradation pathways. GCDH does not form part of a larger enzyme complex but performs its function independently ensuring the proper degradation and metabolism of amino acids. Its activity aids in maintaining metabolic homeostasis preventing the accumulation of harmful intermediates within cells. GCDH may interact with cofactors like flavin adenine dinucleotide (FAD) to carry out its enzymatic activity.

Pathways

GCDH plays a significant role in the lysine and tryptophan metabolism pathways. Within these pathways GCDH helps convert glutaryl-CoA a molecule derived from amino acid catabolism into intermediates ultimately leading to energy production. The enzyme's functionality connects with other mitochondrial enzymes like isovaleryl-CoA dehydrogenase (IVD) which also participates in amino acid breakdown. Proper interaction and coordination within these pathways are essential for maintaining metabolic balance in the cell.

GCDH is closely linked to glutaric aciduria type 1 (GA1) an inborn error of metabolism. This disorder arises from mutations in the GCDH gene leading to insufficient enzyme activity and subsequent buildup of glutaric acid. This accumulation can cause severe neurological damage over time if untreated. GA1 can show genetic connections with other metabolic disorders involving enzymes like medium-chain acyl-CoA dehydrogenase (MCAD). Understanding these interactions helps in diagnosing and managing such metabolic disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA and CO(2) in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism. It uses electron transfer flavoprotein as its electron acceptor. Isoform Short is inactive.
See full target information GCDH
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 15:541 PubMed39080260

2024

HMGA1 promotes the progression of esophageal squamous cell carcinoma by elevating TKT-mediated upregulation of pentose phosphate pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Meng-Jie Liu,Yuan Zhao,Qiu-Tong Li,Xin-Yuan Lei,Kai-Yue He,Jin-Rong Guo,Jing-Yu Yang,Zhen-Hua Yan,Dan-Hui Wu,Lei Zhang,Yong-Ping Jian,Zhi-Xiang Xu
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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