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AB232774

Anti-GCDH/GCD antibody

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(1 Publication)

Rabbit Polyclonal GCDH/GCD antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human GCDH aa 250-450.

View Alternative Names

GCD, GCDH

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDH/GCD antibody (AB232774)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDH/GCD antibody (AB232774)

Formalin-fixed, paraffin-embedded human liver tissue stained for GCDH/GCD using ab232774 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Western blot - Anti-GCDH/GCD antibody (AB232774)
  • WB

Supplier Data

Western blot - Anti-GCDH/GCD antibody (AB232774)

All lanes:

Western blot - Anti-GCDH/GCD antibody (ab232774) at 2 µg/mL

All lanes:

Mouse liver tissue lysate

Predicted band size: 48 kDa

false

Western blot - Anti-GCDH/GCD antibody (AB232774)
  • WB

Supplier Data

Western blot - Anti-GCDH/GCD antibody (AB232774)

All lanes:

Western blot - Anti-GCDH/GCD antibody (ab232774) at 2 µg/mL

All lanes:

Mouse heart tissue lysate

Predicted band size: 48 kDa

false

Western blot - Anti-GCDH/GCD antibody (AB232774)
  • WB

Supplier Data

Western blot - Anti-GCDH/GCD antibody (AB232774)

All lanes:

Western blot - Anti-GCDH/GCD antibody (ab232774) at 2 µg/mL

All lanes:

Recombinant human GCDH/GCD protein

Predicted band size: 48 kDa

false

Western blot - Anti-GCDH/GCD antibody (AB232774)
  • WB

Supplier Data

Western blot - Anti-GCDH/GCD antibody (AB232774)

All lanes:

Western blot - Anti-GCDH/GCD antibody (ab232774) at 2 µg/mL

All lanes:

K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate

Predicted band size: 48 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human GCDH aa 250-450. The exact immunogen used to generate this antibody is proprietary information.

Q92947

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Alternative Products

Primary Antibodies

AB182152

Anti-GCDH/GCD antibody [EPR14864(B)]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-GCDH/GCD antibody [EPR14864(B)] (AB182152)

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Primary Antibodies

AB112998

Anti-GCDH/GCD antibody [3E9BA3BF5]

Flow Cytometry - Anti-GCDH/GCD antibody [3E9BA3BF5] (AB112998)

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Complementary Products

Secondary Antibodies

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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

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Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

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Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

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Proteins & Peptides

AB98118

Recombinant Human GCDH/GCD protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human GCDH/GCD protein (His tag N-Terminus) (AB98118)

  • 0
  • 0
Primary Antibodies

AB18207

Anti-beta III Tubulin antibody - Neuronal Marker

BOND RX™ Validated|Lab Essentials|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody - Neuronal Marker (AB18207)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Macaque monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage buffer
pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GCDH also known as glutaryl-CoA dehydrogenase is an enzyme involved in the catabolic breakdown of specific amino acids. This protein harbors a mass of approximately 45 kDa and functions primarily within the mitochondria. GCDH expression occurs in various tissues including the liver kidney and brain. The enzyme plays a mechanical role by catalyzing the decarboxylation of glutaryl-CoA to crotonyl-CoA facilitating the removal of carbon dioxide in this biochemical process.
Biological function summary

This enzyme acts as an important component in the lysine hydroxylysine and tryptophan degradation pathways. GCDH does not form part of a larger enzyme complex but performs its function independently ensuring the proper degradation and metabolism of amino acids. Its activity aids in maintaining metabolic homeostasis preventing the accumulation of harmful intermediates within cells. GCDH may interact with cofactors like flavin adenine dinucleotide (FAD) to carry out its enzymatic activity.

Pathways

GCDH plays a significant role in the lysine and tryptophan metabolism pathways. Within these pathways GCDH helps convert glutaryl-CoA a molecule derived from amino acid catabolism into intermediates ultimately leading to energy production. The enzyme's functionality connects with other mitochondrial enzymes like isovaleryl-CoA dehydrogenase (IVD) which also participates in amino acid breakdown. Proper interaction and coordination within these pathways are essential for maintaining metabolic balance in the cell.

GCDH is closely linked to glutaric aciduria type 1 (GA1) an inborn error of metabolism. This disorder arises from mutations in the GCDH gene leading to insufficient enzyme activity and subsequent buildup of glutaric acid. This accumulation can cause severe neurological damage over time if untreated. GA1 can show genetic connections with other metabolic disorders involving enzymes like medium-chain acyl-CoA dehydrogenase (MCAD). Understanding these interactions helps in diagnosing and managing such metabolic disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA and CO(2) in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism. It uses electron transfer flavoprotein as its electron acceptor. Isoform Short is inactive.
See full target information GCDH
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Fundamental & clinical pharmacology 39:e13048 PubMed39777430

2025

Fluoxetine-induced downregulation of circMap2k1 signaling cascade to improve neurological function after ischemic stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Langtao He,Hui Zhang,Jian Deng,Yibo He,Zhili Cai,Yitao He
View all publications
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Product promise

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