Rabbit Recombinant Monoclonal GCET1 antibody. Carrier free. Suitable for WB and reacts with Human samples.
Images
![Western blot - Anti-GCET1 antibody [EPR12224] - BSA and Azide free (AB250117), expandable thumbnail](https://content.abcam.com/products/images/gcet1-antibody-epr12224-bsa-and-azide-free-ab250117--western-blot-img160805.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GCET1 antibody [EPR12224] - BSA and Azide free (AB250117)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Predicted | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Predicted | Not recommended | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Protease inhibitor that inhibits trypsin and trypsin-like serine proteases (in vitro). Inhibits plasmin and thrombin with lower efficiency (in vitro).
Alternative names
Serpin A9, Centerin, Germinal center B-cell-expressed transcript 1 protein, UNQ692/PRO1337, SERPINA9, SERPINA11, GCET1
Recommended products
Rabbit Recombinant Monoclonal GCET1 antibody. Carrier free. Suitable for WB and reacts with Human samples.
Alternative names
Serpin A9, Centerin, Germinal center B-cell-expressed transcript 1 protein, UNQ692/PRO1337, SERPINA9, SERPINA11, GCET1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR12224
- Purification technique
Affinity purification
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab250117 is the carrier-free version of Anti-GCET1 antibody [EPR12224] ab179814.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GCET1 or germinal center B-cell expressed transcript 1 is a protein also known as CLL-1. It weighs approximately 43 kDa. This protein primarily appears in germinal center B-cells and shows up in some cases of diffuse large B-cell lymphoma (DLBCL) and follicular lymphoma. It usually marks the cells involved in proliferation processes which are critical in some lymphomas’ pathogenesis and progression.
- Biological function summary
GCET1 participates in the regulation of B-cell proliferation and survival although it is not itself a signaling molecule. It operates mainly within the immune system particularly affecting B-cells. This protein does not typically form part of a larger complex but may influence the activity of other proteins tied to B-cell activation and development aiding in the control of immune responses.
- Pathways
GCET1 shows involvement with B-cell receptor signaling and associated signaling pathways. These pathways are central to lymphocyte development activation and differentiation. It interacts closely with essential signaling proteins like SYK and BLNK. Alterations in these pathways can contribute to improper B-cell functioning and linked immunological abnormalities.
- Associated diseases and disorders
GCET1 often relates to lymphoma particularly diffuse large B-cell lymphoma and follicular lymphoma. The protein GCET1 shows reciprocal connections with other molecules like BCL-6 in these cancers. Understanding GCET1 helps in identifying potential therapeutic targets and aids in diagnosing certain lymphoid malignancies underlining its value in clinical research related to these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - Anti-GCET1 antibody [EPR12224] - BSA and Azide free (ab250117), expandable thumbnail](https://content.abcam.com/products/images/gcet1-antibody-epr12224-bsa-and-azide-free-ab250117--western-blot-img160805.jpg "Western blot - Anti-GCET1 antibody [EPR12224] - BSA and Azide free (ab250117)")
Western blot - Anti-GCET1 antibody [EPR12224] - BSA and Azide free (ab250117)
This data was developed using Anti-GCET1 antibody [EPR12224] ab179814, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-GCET1 antibody [EPR12224] (Anti-GCET1 antibody [EPR12224] ab179814) at 1/1000 dilution
Lane 1: MCF7 cell lysate at 10 µg
Lane 2: Raji cell lysate at 10 µg
Lane 3: Daudi cell lysate at 10 µg
Predicted band size: 47 kDa
Downloads
Product protocols
- Visit the general protocols
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