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AB250117

Anti-GCET1 antibody [EPR12224] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal GCET1 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

GCET1, SERPINA11, UNQ692/PRO1337, SERPINA9, Serpin A9, Centerin, Germinal center B-cell-expressed transcript 1 protein

1 Images
Western blot - Anti-GCET1 antibody [EPR12224] - BSA and Azide free (AB250117)
  • WB

Supplier Data

Western blot - Anti-GCET1 antibody [EPR12224] - BSA and Azide free (AB250117)

This data was developed using ab179814, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-GCET1 antibody [EPR12224] (<a href='/en-us/products/primary-antibodies/gcet1-antibody-epr12224-ab179814'>ab179814</a>) at 1/1000 dilution

Lane 1:

MCF7 cell lysate at 10 µg

Lane 2:

Raji cell lysate at 10 µg

Lane 3:

Daudi cell lysate at 10 µg

Predicted band size: 47 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12224

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

ab250117 is the carrier-free version of ab179814.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GCET1 or germinal center B-cell expressed transcript 1 is a protein also known as CLL-1. It weighs approximately 43 kDa. This protein primarily appears in germinal center B-cells and shows up in some cases of diffuse large B-cell lymphoma (DLBCL) and follicular lymphoma. It usually marks the cells involved in proliferation processes which are critical in some lymphomas’ pathogenesis and progression.
Biological function summary

GCET1 participates in the regulation of B-cell proliferation and survival although it is not itself a signaling molecule. It operates mainly within the immune system particularly affecting B-cells. This protein does not typically form part of a larger complex but may influence the activity of other proteins tied to B-cell activation and development aiding in the control of immune responses.

Pathways

GCET1 shows involvement with B-cell receptor signaling and associated signaling pathways. These pathways are central to lymphocyte development activation and differentiation. It interacts closely with essential signaling proteins like SYK and BLNK. Alterations in these pathways can contribute to improper B-cell functioning and linked immunological abnormalities.

GCET1 often relates to lymphoma particularly diffuse large B-cell lymphoma and follicular lymphoma. The protein GCET1 shows reciprocal connections with other molecules like BCL-6 in these cancers. Understanding GCET1 helps in identifying potential therapeutic targets and aids in diagnosing certain lymphoid malignancies underlining its value in clinical research related to these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Protease inhibitor that inhibits trypsin and trypsin-like serine proteases (in vitro). Inhibits plasmin and thrombin with lower efficiency (in vitro).
See full target information SERPINA9
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 295:12868-12884 PubMed32580945

2020

Adipose mesenchymal stem cell-derived extracellular vesicles containing microRNA-26a-5p target TLR4 and protect against diabetic nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Yurui Duan,Qingyang Luo,Yun Wang,Yali Ma,Fang Chen,Xiaoguang Zhu,Jun Shi
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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