AB185539

Anti-GCET2 antibody [EPR14333-60]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal GCET2 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

GAL, GCET2, GCSAM, Germinal center-associated signaling and motility protein, Germinal center B-cell-expressed transcript 2 protein, Germinal center-associated lymphoma protein, hGAL

2 Images

Flow Cytometry (Intracellular) - Anti-GCET2 antibody [EPR14333-60] (AB185539)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-GCET2 antibody [EPR14333-60] (AB185539)

Intracellular Flow Cytometry analysis of 2% paraformaldehyde fixed Jurkat cells labeling GCET2 with ab185539 at 1/130 dilution (pink) or a rabbit IgG isotype control (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution.

Supplier Data

Western blot - Anti-GCET2 antibody [EPR14333-60] (AB185539)

All lanes:

Western blot - Anti-GCET2 antibody [EPR14333-60] (ab185539) at 1/5000 dilution

Lane 1:

Raji cell lysate at 20 µg

Lane 2:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 21 kDa

Observed band size: 21 kDa

false

Carrier free

Anti-GCET2 antibody [EPR14333-60] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14333-60

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/130", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GCET2 also known as germinal center-expressed transcript 2 or centroblast protein has a molecular mass of approximately 19 kDa. It expresses mainly in germinal center B cells specifically the centroblasts within lymphoid tissues. This protein functions mechanically by having a role in the regulation of B cell differentiation and proliferation. Research demonstrates its expression during specific stages of B cell development indicating an important involvement during immune processes.

Biological function summary

This protein influences the immune response by contributing to the maturation and selection of B cells within germinal centers. Although not known to be a part of any larger complex its expression corresponds with stages critical to adaptive immunity. GCET2 supports the processes that ensure only high-affinity B cell receptors mature fostering a strong and specific immune defense.

Pathways

Researchers place GCET2 in connection with the germinal center reaction a significant pathway in adaptive immunity. This pathway involves interaction with proteins such as Bcl-6 which regulates the formation and maintenance of germinal centers. Another pathway is the somatic hypermutation process critical for antibody diversity where GCET2 may influence the changes in antibody affinity.

Scientists have linked GCET2 to certain conditions like lymphoma where its dysregulated expression may contribute to tumorigenesis. Evidence suggests that abnormalities in GCET2 expression levels correlate with the progression of some lymphoid malignancies. In autoimmune disorders this protein has associations with aberrant immune responses potentially increasing susceptibility to conditions characterized by excessive or uncontrolled immune activity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in the negative regulation of lymphocyte motility. It mediates the migration-inhibitory effects of IL6. Serves as a positive regulator of the RhoA signaling pathway. Enhancement of RhoA activation results in inhibition of lymphocyte and lymphoma cell motility by activation of its downstream effector ROCK. Is a regulator of B-cell receptor signaling, that acts through SYK kinase activation.

See full target information GCSAM

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature immunology 25:873-885 PubMed38553615

2024

Thioredoxin is a metabolic rheostat controlling regulatory B cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hannah F Bradford,Thomas C R McDonnell,Alexander Stewart,Andrew Skelton,Joseph Ng,Zara Baig,Franca Fraternali,Deborah Dunn-Walters,David A Isenberg,Adnan R Khan,Claudio Mauro,Claudia Mauri

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GCET2 antibody [EPR14333-60]

Flow Cytometry (Intracellular) - Anti-GCET2 antibody [EPR14333-60] (AB185539)

Western blot - Anti-GCET2 antibody [EPR14333-60] (AB185539)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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