JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB250826

Anti-GCET2 antibody [EPR14333] - BSA and Azide free

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal GCET2 antibody. Carrier free. Suitable for ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

GAL, GCET2, GCSAM, Germinal center-associated signaling and motility protein, Germinal center B-cell-expressed transcript 2 protein, Germinal center-associated lymphoma protein, hGAL

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-GCET2 antibody [EPR14333] - BSA and Azide free (AB250826)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GCET2 antibody [EPR14333] - BSA and Azide free (AB250826)

This data was developed using ab185231, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of Raji cells labeling GCET2 with ab185231 at 1/500 (red) and DAPI staining (blue). Cells fixed in -20°C Acetone.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCET2 antibody [EPR14333] - BSA and Azide free (AB250826)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCET2 antibody [EPR14333] - BSA and Azide free (AB250826)

This data was developed using ab185231, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human Non Hodgkin's lymphoma tissue labeling GCET2 with ab185231 at 1/2000.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCET2 antibody [EPR14333] - BSA and Azide free (AB250826)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCET2 antibody [EPR14333] - BSA and Azide free (AB250826)

This data was developed using ab185231, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human diffuse large B-cell lymphoma tissue labeling GCET2 with ab185231 at 1/2000.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-GCET2 antibody [EPR14333] - BSA and Azide free (AB250826)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-GCET2 antibody [EPR14333] - BSA and Azide free (AB250826)

This data was developed using ab185231, the same antibody clone in a different buffer formulation.

Intracellular flow cytometrical analysis of Raji cells labeling GCET2 with ab185231 at 1/230 and Goat anti rabbit IgG (FITC) at 1/150 as a secondary antibody compared to a negative control antibody.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14333

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Primary Antibodies

AB79757

Anti-ENO1 + ENO2 + ENO3 antibody [EPR3377] - Neuronal Marker

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-ENO1 + ENO2 + ENO3 antibody [EPR3377] - Neuronal Marker (AB79757)

  • 1
  • 3
Primary Antibodies

AB187646

Anti-Uroplakin III antibody [EPR14420]

RabMAb|Recombinant|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin III antibody [EPR14420] (AB187646)

  • 0
  • 0
Primary Antibodies

AB91652

Anti-LMO2 antibody [EP3257]

RabMAb|Recombinant

Immunoprecipitation - Anti-LMO2 antibody [EP3257] (AB91652)

  • 0
  • 0
Primary Antibodies

AB208020

Anti-Mre11 antibody [EPR21027] - ChIP Grade

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mre11 antibody [EPR21027] - ChIP Grade (AB208020)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
style
left-column

Product details

ab250826 is the carrier-free version of ab185231.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GCET2 also known as germinal center-expressed transcript 2 or centroblast protein has a molecular mass of approximately 19 kDa. It expresses mainly in germinal center B cells specifically the centroblasts within lymphoid tissues. This protein functions mechanically by having a role in the regulation of B cell differentiation and proliferation. Research demonstrates its expression during specific stages of B cell development indicating an important involvement during immune processes.
Biological function summary

This protein influences the immune response by contributing to the maturation and selection of B cells within germinal centers. Although not known to be a part of any larger complex its expression corresponds with stages critical to adaptive immunity. GCET2 supports the processes that ensure only high-affinity B cell receptors mature fostering a strong and specific immune defense.

Pathways

Researchers place GCET2 in connection with the germinal center reaction a significant pathway in adaptive immunity. This pathway involves interaction with proteins such as Bcl-6 which regulates the formation and maintenance of germinal centers. Another pathway is the somatic hypermutation process critical for antibody diversity where GCET2 may influence the changes in antibody affinity.

Scientists have linked GCET2 to certain conditions like lymphoma where its dysregulated expression may contribute to tumorigenesis. Evidence suggests that abnormalities in GCET2 expression levels correlate with the progression of some lymphoid malignancies. In autoimmune disorders this protein has associations with aberrant immune responses potentially increasing susceptibility to conditions characterized by excessive or uncontrolled immune activity.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Involved in the negative regulation of lymphocyte motility. It mediates the migration-inhibitory effects of IL6. Serves as a positive regulator of the RhoA signaling pathway. Enhancement of RhoA activation results in inhibition of lymphocyte and lymphoma cell motility by activation of its downstream effector ROCK. Is a regulator of B-cell receptor signaling, that acts through SYK kinase activation.
See full target information GCSAM
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of radiation research 64:833-841 PubMed37439405

2023

Expression of TMEM59L associated with radiosensitive in glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Dezhi Gao,Peng Wang,Lin Zhi,Shibin Sun,Xiaoguang Qiu,Yanwei Liu
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com