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AB69962

Anti-GCH1 antibody

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(1 Publication)

Mouse Polyclonal GCH1 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human GCH1.

View Alternative Names

DYT5, GCH, GCH1, GTP cyclohydrolase 1, GTP cyclohydrolase I, GTP-CH-I

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-GCH1 antibody (AB69962)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GCH1 antibody (AB69962)

Immunocytochemistry of HeLa cells staining GCH1 using ab69962 at 10μg/ml.

Western blot - Anti-GCH1 antibody (AB69962)
  • WB

Unknown

Western blot - Anti-GCH1 antibody (AB69962)

All lanes:

Western blot - Anti-GCH1 antibody (ab69962) at 1/500 dilution

Lane 1:

GCH1 transfected 293T cell lysate at 25 µg

Lane 2:

Non-transfected 293T cell lysate at 25 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Predicted band size: 28 kDa

Observed band size: 28 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human GCH1.

P30793

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Alternative Products

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  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Positively regulates nitric oxide synthesis in umbilical vein endothelial cells (HUVECs). May be involved in dopamine synthesis. May modify pain sensitivity and persistence. Isoform GCH-1 is the functional enzyme, the potential function of the enzymatically inactive isoforms remains unknown.
See full target information GCH1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

American journal of physiology. Lung cellular and 307:L987-97 PubMed25326583

2014

Nox2-dependent glutathionylation of endothelial NOS leads to uncoupled superoxide production and endothelial barrier dysfunction in acute lung injury.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Wu,William S Szczepaniak,Sruti Shiva,Huanbo Liu,Yinna Wang,Ling Wang,Ying Wang,Eric E Kelley,Alex F Chen,Mark T Gladwin,Bryan J McVerry
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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