Anti-GCH1 antibody [EPR27331-30] - BSA and Azide free (ab307508)

Rabbit Recombinant Monoclonal GCH1 antibody. Carrier free. Suitable for IP, IHC-P, WB and reacts with Mouse, Rat, Human samples.

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Images

Western blot - Anti-GCH1 antibody [EPR27331-30] - BSA and Azide free (AB307508), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	Flow Cyt (Intra)	ICC/IF	IHC-Fr	IHC-P	WB
Human	Expected	Not recommended	Not recommended	Not recommended	Tested	Tested
Mouse	Tested	Not recommended	Not recommended	Not recommended	Tested	Tested
Rat	Expected	Not recommended	Not recommended	Not recommended	Tested	Tested

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat, Human	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat, Mouse, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info -	Notes -

Target data

See full target information GCH1

Function

Positively regulates nitric oxide synthesis in umbilical vein endothelial cells (HUVECs). May be involved in dopamine synthesis. May modify pain sensitivity and persistence. Isoform GCH-1 is the functional enzyme, the potential function of the enzymatically inactive isoforms remains unknown.

Alternative names

DYT5, GCH, GCH1, GTP cyclohydrolase 1, GTP cyclohydrolase I, GTP-CH-I

Recommended products

Rabbit Recombinant Monoclonal GCH1 antibody. Carrier free. Suitable for IP, IHC-P, WB and reacts with Mouse, Rat, Human samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR27331-30
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

12 product images

Western blot - Anti-GCH1 antibody [EPR27331-30] - BSA and Azide free (ab307508)
This data was developed using 307507, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: Negative control: skeletal muscle (PMID:22798524)
180 seconds
Exposure time:
All lanes: Western blot - Anti-GCH1 antibody [EPR27331-30] (Anti-GCH1 antibody [EPR27331-30] ab307507) at 1/1000 dilution
Lane 1: Mouse liver tissue lysate 20 μg
Lane 2: Mouse hypothalamus tissue lysate 20 μg
Lane 3: Mouse skeletal muscle tissue lysate 20 μg
Lane 4: Rat liver tissue lysate 20 μg
Lane 5: Rat hypothalamus tissue lysate 20 μg
Lane 6: Rat skeletal muscle tissue lysate 20 μg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 27 kDa
Exposure time: 180s
Western blot - Anti-GCH1 antibody [EPR27331-30] - BSA and Azide free (ab307508)
This data was developed using 307507, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: Negative control: K562 (PMID:32778843)

Exposure time:
All lanes: Western blot - Anti-GCH1 antibody [EPR27331-30] (Anti-GCH1 antibody [EPR27331-30] ab307507) at 1/1000 dilution
Lane 1: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate 20 μg
Lane 2: K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate 20 μg
Lane 3: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate 20 μg
Lane 4: N9 (mouse microglia) whole cell lysate 20 μg
Lane 5: PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate 20 μg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 27 kDa
Exposure time: 37s
Immunoprecipitation - Anti-GCH1 antibody [EPR27331-30] - BSA and Azide free (ab307508)
This data was developed using Anti-GCH1 antibody [EPR27331-30] ab307507, the same antibody clone in a different buffer formulation.
GCH1 was immunoprecipitated from 0.35 mg Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate 10 ug with Anti-GCH1 antibody [EPR27331-30] ab307507 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-GCH1 antibody [EPR27331-30] ab307507 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution. Lane 1: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate 10 ug
Lane 2: ABAB307507 IP in Neuro-2a whole cell lysate
Lane 3:RABbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-GCH1 antibody [EPR27331-30] ab307507 in Neuro-2a whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 6 seconds
All lanes: Immunoprecipitation - Anti-GCH1 antibody [EPR27331-30] (Anti-GCH1 antibody [EPR27331-30] ab307507) at 1/1000 dilution
Lane 1: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate 10 μg
Lane 2: Neuro-2a whole cell lysate
Lane 3: Immunoprecipitation - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730)
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Observed band size: 27 kDa
Exposure time: 6s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCH1 antibody [EPR27331-30] - BSA and Azide free (ab307508)
This data was developed using Anti-GCH1 antibody [EPR27331-30] ab307507, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling GCH1 with Anti-GCH1 antibody [EPR27331-30] ab307507 at 1/1000 (0.522 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Negative control: No staining on rat skeletal muscle.The section was incubated with Anti-GCH1 antibody [EPR27331-30] ab307507 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCH1 antibody [EPR27331-30] - BSA and Azide free (ab307508)
This data was developed using Anti-GCH1 antibody [EPR27331-30] ab307507, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscl tissue labeling GCH1 with Anti-GCH1 antibody [EPR27331-30] ab307507 at 1/1000 (0.522 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Negative control: No staining on mouse skeletal muscle.The section was incubated with Anti-GCH1 antibody [EPR27331-30] ab307507 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCH1 antibody [EPR27331-30] - BSA and Azide free (ab307508)
This data was developed using Anti-GCH1 antibody [EPR27331-30] ab307507, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human skeletal muscl tissue labeling GCH1 with Anti-GCH1 antibody [EPR27331-30] ab307507 at 1/500 (1.044 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Negative control: No staining on human skeletal muscle.The section was incubated with Anti-GCH1 antibody [EPR27331-30] ab307507 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCH1 antibody [EPR27331-30] - BSA and Azide free (ab307508)
This data was developed using Anti-GCH1 antibody [EPR27331-30] ab307507, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat pancreas tissue labeling GCH1 with Anti-GCH1 antibody [EPR27331-30] ab307507 at 1/1000 (0.522 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Positive staining on rat pancreatic islets.The section was incubated with Anti-GCH1 antibody [EPR27331-30] ab307507 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCH1 antibody [EPR27331-30] - BSA and Azide free (ab307508)
This data was developed using Anti-GCH1 antibody [EPR27331-30] ab307507, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling GCH1 with Anti-GCH1 antibody [EPR27331-30] ab307507 at 1/1000 (0.522 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Positive staining on scattered cells in rat colon.The section was incubated with Anti-GCH1 antibody [EPR27331-30] ab307507 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCH1 antibody [EPR27331-30] - BSA and Azide free (ab307508)
This data was developed using Anti-GCH1 antibody [EPR27331-30] ab307507, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling GCH1 with Anti-GCH1 antibody [EPR27331-30] ab307507 at 1/1000 (0.522 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Positive staining on scattered cells in mouse stomach.The section was incubated with Anti-GCH1 antibody [EPR27331-30] ab307507 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCH1 antibody [EPR27331-30] - BSA and Azide free (ab307508)
This data was developed using Anti-GCH1 antibody [EPR27331-30] ab307507, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse midbrain tissue labeling GCH1 with Anti-GCH1 antibody [EPR27331-30] ab307507 at 1/1000 (0.522 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Positive staining on neurons of mouse midbrain (PMID: 10907721). The section was incubated with Anti-GCH1 antibody [EPR27331-30] ab307507 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCH1 antibody [EPR27331-30] - BSA and Azide free (ab307508)
This data was developed using Anti-GCH1 antibody [EPR27331-30] ab307507, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling GCH1 with Anti-GCH1 antibody [EPR27331-30] ab307507 at 1/500 (1.044 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Positive staining on human pancreatic islets.The section was incubated with Anti-GCH1 antibody [EPR27331-30] ab307507 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCH1 antibody [EPR27331-30] - BSA and Azide free (ab307508)
This data was developed using Anti-GCH1 antibody [EPR27331-30] ab307507, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling GCH1 with Anti-GCH1 antibody [EPR27331-30] ab307507 at 1/500 (1.044 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Positive staining on scattered cells in human colon (PMID: 22753274).The section was incubated with Anti-GCH1 antibody [EPR27331-30] ab307507 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins