Anti-GCH1 antibody [EPR27331-30] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCH1 antibody [EPR27331-30] - BSA and Azide free (AB307508)

This data was developed using ab307507, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling GCH1 with ab307507 at 1/500 (1.044 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Positive staining on scattered cells in human colon (PMID : 22753274).The section was incubated with ab307507 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCH1 antibody [EPR27331-30] - BSA and Azide free (AB307508)

This data was developed using ab307507, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human skeletal muscl tissue labeling GCH1 with ab307507 at 1/500 (1.044 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Negative control : No staining on human skeletal muscle.The section was incubated with ab307507 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCH1 antibody [EPR27331-30] - BSA and Azide free (AB307508)

This data was developed using ab307507, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling GCH1 with ab307507 at 1/500 (1.044 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Positive staining on human pancreatic islets.The section was incubated with ab307507 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCH1 antibody [EPR27331-30] - BSA and Azide free (AB307508)

This data was developed using ab307507, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling GCH1 with ab307507 at 1/1000 (0.522 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Positive staining on scattered cells in rat colon.The section was incubated with ab307507 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCH1 antibody [EPR27331-30] - BSA and Azide free (AB307508)

This data was developed using ab307507, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling GCH1 with ab307507 at 1/1000 (0.522 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Negative control : No staining on rat skeletal muscle.The section was incubated with ab307507 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCH1 antibody [EPR27331-30] - BSA and Azide free (AB307508)

This data was developed using ab307507, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscl tissue labeling GCH1 with ab307507 at 1/1000 (0.522 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Negative control : No staining on mouse skeletal muscle.The section was incubated with ab307507 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCH1 antibody [EPR27331-30] - BSA and Azide free (AB307508)

This data was developed using ab307507, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat pancreas tissue labeling GCH1 with ab307507 at 1/1000 (0.522 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Positive staining on rat pancreatic islets.The section was incubated with ab307507 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCH1 antibody [EPR27331-30] - BSA and Azide free (AB307508)

This data was developed using ab307507, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse midbrain tissue labeling GCH1 with ab307507 at 1/1000 (0.522 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Positive staining on neurons of mouse midbrain (PMID : 10907721). The section was incubated with ab307507 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCH1 antibody [EPR27331-30] - BSA and Azide free (AB307508)

This data was developed using ab307507, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling GCH1 with ab307507 at 1/1000 (0.522 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Positive staining on scattered cells in mouse stomach.The section was incubated with ab307507 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IP

Supplier Data

Immunoprecipitation - Anti-GCH1 antibody [EPR27331-30] - BSA and Azide free (AB307508)

This data was developed using ab307507, the same antibody clone in a different buffer formulation. GCH1 was immunoprecipitated from 0.35 mg Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate 10 ug with ab307507 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307507 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate 10 ug Lane 2 : ab307507 IP in Neuro-2a whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab307507 in Neuro-2a whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 6 seconds

All lanes:

Immunoprecipitation - Anti-GCH1 antibody [EPR27331-30] (<a href='/en-us/products/primary-antibodies/gch1-antibody-epr27331-30-ab307507'>ab307507</a>) at 1/1000 dilution

Lane 1:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate 10 μg

Lane 2:

Neuro-2a whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/en-us/products/primary-antibodies/gch1-antibody-epr27331-30-ab307507'>ab307507</a> in Neuro-2a whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 27 kDa

false

Exposure time: 6s

• WB

Supplier Data

Western blot - Anti-GCH1 antibody [EPR27331-30] - BSA and Azide free (AB307508)

This data was developed using 307507, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : Negative control : K562 (PMID : 32778843) Exposure time :

All lanes:

Western blot - Anti-GCH1 antibody [EPR27331-30] (<a href='/en-us/products/primary-antibodies/gch1-antibody-epr27331-30-ab307507'>ab307507</a>) at 1/1000 dilution

Lane 1:

SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate 20 μg

Lane 2:

K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate 20 μg

Lane 3:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate 20 μg

Lane 4:

N9 (mouse microglia) whole cell lysate 20 μg

Lane 5:

PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate 20 μg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 27 kDa

false

Exposure time: 37s

• WB

Supplier Data

Western blot - Anti-GCH1 antibody [EPR27331-30] - BSA and Azide free (AB307508)

This data was developed using 307507, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : Negative control : skeletal muscle (PMID : 22798524) 180 seconds Exposure time :

All lanes:

Western blot - Anti-GCH1 antibody [EPR27331-30] (<a href='/en-us/products/primary-antibodies/gch1-antibody-epr27331-30-ab307507'>ab307507</a>) at 1/1000 dilution

Lane 1:

Mouse liver tissue lysate 20 μg

Lane 2:

Mouse hypothalamus tissue lysate 20 μg

Lane 3:

Mouse skeletal muscle tissue lysate 20 μg

Lane 4:

Rat liver tissue lysate 20 μg

Lane 5:

Rat hypothalamus tissue lysate 20 μg

Lane 6:

Rat skeletal muscle tissue lysate 20 μg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 27 kDa

false

Exposure time: 180s