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AB154120

Anti-GCKR antibody

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(1 Publication)

Rabbit Polyclonal GCKR antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human GCKR aa 50-500.

View Alternative Names

Glucokinase regulatory protein, GKRP, Glucokinase regulator, GCKR

2 Images
Western blot - Anti-GCKR antibody (AB154120)
  • WB

Unknown

Western blot - Anti-GCKR antibody (AB154120)

7.5% SDS PAGE

All lanes:

Western blot - Anti-GCKR antibody (ab154120) at 1/1000 dilution

Lane 1:

293T whole cell lysate at 30 µg

Lane 2:

A431 whole cell lysate at 30 µg

Predicted band size: 69 kDa

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Western blot - Anti-GCKR antibody (AB154120)
  • WB

Unknown

Western blot - Anti-GCKR antibody (AB154120)

7.5% SDS PAGE

All lanes:

Western blot - Anti-GCKR antibody (ab154120) at 1/3000 dilution

All lanes:

Mouse liver whole cell lysate at 50 µg

Predicted band size: 69 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human GCKR aa 50-500. The exact immunogen used to generate this antibody is proprietary information.

Q14397

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulates glucokinase (GCK) by forming an inactive complex with this enzyme (PubMed : 23621087, PubMed : 23733961). Acts by promoting GCK recruitment to the nucleus, possibly to provide a reserve of GCK that can be quickly released in the cytoplasm after a meal (PubMed : 10456334). The affinity of GCKR for GCK is modulated by fructose metabolites : GCKR with bound fructose 6-phosphate has increased affinity for GCK, while GCKR with bound fructose 1-phosphate has strongly decreased affinity for GCK and does not inhibit GCK activity (PubMed : 23621087, PubMed : 23733961).
See full target information GCKR
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular physiology 234:927-939 PubMed30144066

2018

Downregulated microRNA-340-5p promotes proliferation and inhibits apoptosis of chondrocytes in osteoarthritis mice through inhibiting the extracellular signal-regulated kinase signaling pathway by negatively targeting the FMOD gene.

Applications

Unspecified application

Species

Unspecified reactive species

Weikai Zhang,Peng Cheng,Weihua Hu,Weifeng Yin,Fengjing Guo,Anmin Chen,Hui Huang
View all publications
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Product promise

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