Rabbit Polyclonal GCKR antibody. Suitable for WB and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human GCKR aa 50-500.
IgG
Rabbit
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Liquid
Polyclonal
WB | |
---|---|
Human | Tested |
Mouse | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Regulates glucokinase (GCK) by forming an inactive complex with this enzyme (PubMed:23621087, PubMed:23733961). Acts by promoting GCK recruitment to the nucleus, possibly to provide a reserve of GCK that can be quickly released in the cytoplasm after a meal (PubMed:10456334). The affinity of GCKR for GCK is modulated by fructose metabolites: GCKR with bound fructose 6-phosphate has increased affinity for GCK, while GCKR with bound fructose 1-phosphate has strongly decreased affinity for GCK and does not inhibit GCK activity (PubMed:23621087, PubMed:23733961).
Glucokinase regulatory protein, GKRP, Glucokinase regulator, GCKR
Rabbit Polyclonal GCKR antibody. Suitable for WB and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human GCKR aa 50-500.
Glucokinase regulatory protein, GKRP, Glucokinase regulator, GCKR
IgG
Rabbit
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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7.5% SDS PAGE
All lanes: Western blot - Anti-GCKR antibody (ab154120) at 1/1000 dilution
Lane 1: 293T whole cell lysate at 30 µg
Lane 2: A431 whole cell lysate at 30 µg
Predicted band size: 69 kDa
7.5% SDS PAGE
All lanes: Western blot - Anti-GCKR antibody (ab154120) at 1/3000 dilution
All lanes: Mouse liver whole cell lysate at 50 µg
Predicted band size: 69 kDa
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