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AB279376

Anti-GCKR antibody [OTI1E7]

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Mouse Monoclonal GCKR antibody. Suitable for WB and reacts with Transfected cell lysate, Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human GCKR.

View Alternative Names

Glucokinase regulatory protein, GKRP, Glucokinase regulator, GCKR

2 Images
Western blot - Anti-GCKR antibody [OTI1E7] (AB279376)
  • WB

Supplier Data

Western blot - Anti-GCKR antibody [OTI1E7] (AB279376)

All lanes:

Western blot - Anti-GCKR antibody [OTI1E7] (ab279376) at 1/2000 dilution

Lane 1:

HepG2 cell extract at 35 µg

Lane 2:

HeLa cell extract at 35 µg

Lane 3:

SVT2 cell extract at 35 µg

Lane 4:

A549 cell extract at 35 µg

Lane 5:

COS-7 cell extract at 35 µg

Lane 6:

Jurkat cell extract at 35 µg

Lane 7:

MDCK cell extract at 35 µg

Lane 8:

PC12 cell extract at 35 µg

Lane 9:

MCF7 cell extract at 35 µg

Predicted band size: 69 kDa

false

Western blot - Anti-GCKR antibody [OTI1E7] (AB279376)
  • WB

Supplier Data

Western blot - Anti-GCKR antibody [OTI1E7] (AB279376)

All lanes:

Western blot - Anti-GCKR antibody [OTI1E7] (ab279376) at 1/2000 dilution

Lane 1:

HEK293T cells transfected with the pCMV6-ENTRY control at 5 µg

Lane 2:

HEK293T cells were transfected with the pCMV6-ENTRY GCKR cDNA for 48 hrs at 5 µg

Predicted band size: 69 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI1E7

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human GCKR.

Q14397

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Secondary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "African green monkey": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "<p></p>" }, "Transfected cell lysate": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulates glucokinase (GCK) by forming an inactive complex with this enzyme (PubMed : 23621087, PubMed : 23733961). Acts by promoting GCK recruitment to the nucleus, possibly to provide a reserve of GCK that can be quickly released in the cytoplasm after a meal (PubMed : 10456334). The affinity of GCKR for GCK is modulated by fructose metabolites : GCKR with bound fructose 6-phosphate has increased affinity for GCK, while GCKR with bound fructose 1-phosphate has strongly decreased affinity for GCK and does not inhibit GCK activity (PubMed : 23621087, PubMed : 23733961).
See full target information GCKR
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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