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AB190685

Anti-GCLC antibody [EP13475]

5

(7 Reviews)

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(70 Publications)

Rabbit Recombinant Monoclonal GCLC antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 70 publications.

View Alternative Names

GLCL, GLCLC, GCLC, Glutamate--cysteine ligase catalytic subunit, GCS heavy chain, Gamma-ECS, Gamma-glutamylcysteine synthetase

3 Images
Immunoprecipitation - Anti-GCLC antibody [EP13475] (AB190685)
  • IP

Unknown

Immunoprecipitation - Anti-GCLC antibody [EP13475] (AB190685)

ab190685 at 1/20 dilution immunoprecipitating GCLC in Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates.
Lane 1 (input) : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10μg
Lane 2 (+) : Jurkat whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab190685 in Jurkat whole cell lysate
For western blotting, ab227648 at 1/500 dilution and VeriBlot for IP Detection Reagent (HRP)(ab131366) at 1/50000 dilution were used.

Blocking and diluting buffer : 5% NFDM /TBST.

All lanes:

Immunoprecipitation - Anti-GCLC antibody [EP13475] (ab190685)

Predicted band size: 73 kDa

Observed band size: 73 kDa

false

Western blot - Anti-GCLC antibody [EP13475] (AB190685)
  • WB

Unknown

Western blot - Anti-GCLC antibody [EP13475] (AB190685)

Blocking/diluting buffer and concentation : 5% NFDM/TBST

Exposure time lane 1 : 80 seconds

Exposure time lane 2 : 160 seconds

All lanes:

Western blot - Anti-GCLC antibody [EP13475] (ab190685) at 1/5000 dilution

Lane 1:

Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at 15 µg

Lane 2:

C6 (Rat glial tumor glial cell) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 73 kDa

Observed band size: 73 kDa

false

Western blot - Anti-GCLC antibody [EP13475] (AB190685)
  • WB

Unknown

Western blot - Anti-GCLC antibody [EP13475] (AB190685)

Blocking/diluting buffer and concentration : 5% NFDM/TBST

Exposure time : 180 seconds

All lanes:

Western blot - Anti-GCLC antibody [EP13475] (ab190685) at 1/2000 dilution

All lanes:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 73 kDa

Observed band size: 73 kDa

false

  • Carrier free

    Anti-GCLC antibody [EP13475] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP13475

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/20", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/20000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/20000", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/20000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the ATP-dependent ligation of L-glutamate and L-cysteine and participates in the first and rate-limiting step in glutathione biosynthesis.
See full target information GCLC
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Publications (70)

Recent publications for all applications. Explore the full list and refine your search

Cell death and differentiation 32:1679-1690 PubMed40188196

2025

GCLC desuccinylation regulated by oxidative stress protects human cancer cells from ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Zixiang Chen,Kaifeng Niu,Mengge Li,Yuchun Deng,Ji Zhang,Di Wei,Jiaqi Wang,Yongliang Zhao

Redox biology 79:103479 PubMed39742570

2025

NSUN2 lactylation drives cancer cell resistance to ferroptosis through enhancing GCLC-dependent glutathione synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Kaifeng Niu,Zixiang Chen,Mengge Li,Guannan Ma,Yuchun Deng,Ji Zhang,Di Wei,Jiaqi Wang,Yongliang Zhao

Plants (Basel, Switzerland) 13: PubMed39519981

2024

Daphnetin Protects Schwann Cells Against High-Glucose-Induced Oxidative Injury by Modulating the Nuclear Factor Erythroid 2-Related Factor 2/Glutamate-Cysteine Ligase Catalytic Subunit Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chih-Yuan Ko,Run-Tian Meng,Chung-Hsin Wu,Thi Kim Ngan Nguyen,Yu-En Chen,James Swi-Bea Wu,Wen-Chung Huang,Szu-Chuan Shen

Molecular medicine (Cambridge, Mass.) 30:198 PubMed39497026

2024

Compound 7 regulates microglia polarization and attenuates radiation-induced myelopathy via the Nrf2 signaling pathway in vivo and in vitro studies.

Applications

Unspecified application

Species

Unspecified reactive species

Han Wu,Jianping Wu,Jianzhuo Jiang,Zeyu Qian,Shuang Yang,Yanze Sun,Hongxia Cui,Shengwen Li,Peng Zhang,Zhiqiang Zhou

Phytotherapy research : PTR 38:5441-5457 PubMed39289784

2024

Geniposidic Acid Attenuates Chronic Tubulointerstitial Nephropathy Through Regulation of the NF-ƙB/Nrf2 Pathway Via Aryl Hydrocarbon Receptor Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yan-Ni Wang,Xiao-Jun Li,Wen-Feng Wang,Liang Zou,Hua Miao,Ying-Yong Zhao

Foods (Basel, Switzerland) 13: PubMed39123631

2024

Synergistic and Antagonistic Activity of Selected Dietary Phytochemicals against Oxidative Stress-Induced Injury in Cardiac H9c2 Cells via the Nrf2 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jingwen Yu,Xiangwei Xiao,Baiying Chen,Zeyuan Deng,Xuan Chen,Yawei Fan,Hongyan Li

Antioxidants (Basel, Switzerland) 13: PubMed38671839

2024

Selenium Deficiency Exacerbates Hyperoxia-Induced Lung Injury in Newborn C3H/HeN Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Lora C Bailey-Downs,Laura G Sherlock,Michaela N Crossley,Aristides Rivera Negron,Paul T Pierce,Shirley Wang,Hua Zhong,Cynthia Carter,Kathryn Burge,Jeffrey V Eckert,Lynette K Rogers,Peter F Vitiello,Trent E Tipple

Science advances 10:eadl1088 PubMed38669339

2024

Prevention of age-related truncation of γ-glutamylcysteine ligase catalytic subunit (GCLC) delays cataract formation.

Applications

Unspecified application

Species

Unspecified reactive species

Zongbo Wei,Caili Hao,Kazi Rafsan Radeen,Ramkumar Srinivasagan,Jian-Kang Chen,Shruti Sharma,Meghan E McGee-Lawrence,Mark W Hamrick,Vincent M Monnier,Xingjun Fan

Science advances 10:eadk7329 PubMed38489367

2024

Treatment with siRNAs is commonly associated with GPX4 up-regulation and target knockdown-independent sensitization to ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Anne von Mässenhausen,Marlena Nastassja Schlecht,Kristina Beer,Francesca Maremonti,Wulf Tonnus,Alexia Belavgeni,Shubhangi Gavali,Karolin Flade,Joel S Riley,Nadia Zamora Gonzalez,Anne Brucker,Jorunn Naila Becker,Mirela Tmava,Claudia Meyer,Mirko Peitzsch,Christian Hugo,Florian Gembardt,Jose Pedro Friedmann Angeli,Stefan R Bornstein,Stephen W G Tait,Andreas Linkermann

JCI insight 9: PubMed38329125

2024

Cold stress-induced ferroptosis in liver sinusoidal endothelial cells determines liver transplant injury and outcomes.

Applications

Unspecified application

Species

Unspecified reactive species

Hidenobu Kojima,Hirofumi Hirao,Kentaro Kadono,Takahiro Ito,Siyuan Yao,Taylor Torgerson,Kenneth J Dery,Hiroaki Kitajima,Takahiro Ogawa,Fady M Kaldas,Douglas G Farmer,Jerzy W Kupiec-Weglinski
View all publications
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Product promise

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