Anti-GCLC antibody [EP13475] - BSA and Azide free

• IP

Unknown

Immunoprecipitation - Anti-GCLC antibody [EP13475] - BSA and Azide free (AB240312)

ab190685 at 1/20 dilution immunoprecipitating GCLC in Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates.
Lane 1 (input) : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10μg
Lane 2 (+) : Jurkat whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab190685 in Jurkat whole cell lysate
For western blotting, ab227648 at 1/500 dilution and VeriBlot for IP Detection Reagent (HRP)(ab131366) at 1/50000 dilution were used.

Blocking and diluting buffer : 5% NFDM /TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab190685).

All lanes:

Immunoprecipitation - Anti-GCLC antibody [EP13475] (<a href='/en-us/products/primary-antibodies/gclc-antibody-ep13475-ab190685'>ab190685</a>)

Predicted band size: 73 kDa

Observed band size: 73 kDa

false

• WB

Unknown

Western blot - Anti-GCLC antibody [EP13475] - BSA and Azide free (AB240312)

Blocking/diluting buffer and concentation : 5% NFDM/TBST

Exposure time lane 1 : 80 seconds

Exposure time lane 2 : 160 seconds

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab190685).

All lanes:

Western blot - Anti-GCLC antibody [EP13475] (<a href='/en-us/products/primary-antibodies/gclc-antibody-ep13475-ab190685'>ab190685</a>) at 1/5000 dilution

Lane 1:

Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at 15 µg

Lane 2:

C6 (Rat glial tumor glial cell) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 73 kDa

Observed band size: 73 kDa

false

• WB

Unknown

Western blot - Anti-GCLC antibody [EP13475] - BSA and Azide free (AB240312)

Blocking/diluting buffer and concentration : 5% NFDM/TBST

Exposure time : 180 seconds

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab190685).

All lanes:

Western blot - Anti-GCLC antibody [EP13475] (<a href='/en-us/products/primary-antibodies/gclc-antibody-ep13475-ab190685'>ab190685</a>) at 1/2000 dilution

All lanes:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 73 kDa

Observed band size: 73 kDa

false