JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB240312

Anti-GCLC antibody [EP13475] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal GCLC antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse, Rat samples.

View Alternative Names

GLCL, GLCLC, GCLC, Glutamate--cysteine ligase catalytic subunit, GCS heavy chain, Gamma-ECS, Gamma-glutamylcysteine synthetase

3 Images
Western blot - Anti-GCLC antibody [EP13475] - BSA and Azide free (AB240312)
  • WB

Unknown

Western blot - Anti-GCLC antibody [EP13475] - BSA and Azide free (AB240312)

Blocking/diluting buffer and concentation : 5% NFDM/TBST

Exposure time lane 1 : 80 seconds

Exposure time lane 2 : 160 seconds

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab190685).

All lanes:

Western blot - Anti-GCLC antibody [EP13475] (<a href='/en-us/products/primary-antibodies/gclc-antibody-ep13475-ab190685'>ab190685</a>) at 1/5000 dilution

Lane 1:

Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at 15 µg

Lane 2:

C6 (Rat glial tumor glial cell) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 73 kDa

Observed band size: 73 kDa

false

Immunoprecipitation - Anti-GCLC antibody [EP13475] - BSA and Azide free (AB240312)
  • IP

Unknown

Immunoprecipitation - Anti-GCLC antibody [EP13475] - BSA and Azide free (AB240312)

ab190685 at 1/20 dilution immunoprecipitating GCLC in Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates.
Lane 1 (input) : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10μg
Lane 2 (+) : Jurkat whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab190685 in Jurkat whole cell lysate
For western blotting, ab227648 at 1/500 dilution and VeriBlot for IP Detection Reagent (HRP)(ab131366) at 1/50000 dilution were used.

Blocking and diluting buffer : 5% NFDM /TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab190685).

All lanes:

Immunoprecipitation - Anti-GCLC antibody [EP13475] (<a href='/en-us/products/primary-antibodies/gclc-antibody-ep13475-ab190685'>ab190685</a>)

Predicted band size: 73 kDa

Observed band size: 73 kDa

false

Western blot - Anti-GCLC antibody [EP13475] - BSA and Azide free (AB240312)
  • WB

Unknown

Western blot - Anti-GCLC antibody [EP13475] - BSA and Azide free (AB240312)

Blocking/diluting buffer and concentration : 5% NFDM/TBST

Exposure time : 180 seconds

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab190685).

All lanes:

Western blot - Anti-GCLC antibody [EP13475] (<a href='/en-us/products/primary-antibodies/gclc-antibody-ep13475-ab190685'>ab190685</a>) at 1/2000 dilution

All lanes:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 73 kDa

Observed band size: 73 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP13475

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
style
left-column

You may be interested in:

AB102530

Glutathione Peroxidase Assay Kit (Colorimetric)

5

2 Reviews

View product

We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

style
left-column

Product details

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Catalyzes the ATP-dependent ligation of L-glutamate and L-cysteine and participates in the first and rate-limiting step in glutathione biosynthesis.
See full target information GCLC
style
left-column
style
left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
style
left-column
style
right-column

Associated Products

Select an associated product type
Alternative Product
Primary Antibodies

AB181839

Anti-GCLC antibody [EPR13476(B)]

primary-antibodies

gclc-antibody-epr13476b-ab181839

3

(1 reviews)

Alternative Product
Primary Antibodies

AB240379

Anti-GCLC antibody [EPR20078] - BSA and Azide free

primary-antibodies

gclc-antibody-epr20078-bsa-and-azide-free-ab240379

5

(1 reviews)

Alternative Product
Primary Antibodies

AB207777

Anti-GCLC antibody [EPR20078]

primary-antibodies

gclc-antibody-epr20078-ab207777

0

(0 reviews)

style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com