Rabbit Recombinant Monoclonal GCLC antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.
Images
![Western blot - Anti-GCLC antibody [EPR13476(B)] (AB181839), expandable thumbnail](https://content.abcam.com/products/images/gclc-antibody-epr13476b-ab181839--western-blot-img85831.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GCLC antibody [EPR13476(B)] (AB181839)")
![Western blot - Anti-GCLC antibody [EPR13476(B)] (AB181839), expandable thumbnail](https://content.abcam.com/products/images/gclc-antibody-epr13476b-ab181839--western-blot-img124459.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GCLC antibody [EPR13476(B)] (AB181839)")
Publications
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- American journal of cancer research 11:2911-29272021Glutamate-cysteine ligase catalytic subunit as a therapeutic target in acute myeloid leukemia and solid tumors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChiou-Hong Lin,John P Vu,Chen-Yen Yang,Mint Sirisawad,Chun-Te Chen,Hung Dao,Jing Liu,Xuan Ma,Chin Pan,Joseph Cefalu,Chris Tse,Erica Jackson,Hsu-Ping KuoPubMed 34249435
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Rat | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Catalyzes the ATP-dependent ligation of L-glutamate and L-cysteine and participates in the first and rate-limiting step in glutathione biosynthesis.
Alternative names
GLCL, GLCLC, GCLC, Glutamate--cysteine ligase catalytic subunit, GCS heavy chain, Gamma-ECS, Gamma-glutamylcysteine synthetase
Recommended products
Rabbit Recombinant Monoclonal GCLC antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR13476(B)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-GCLC antibody [EPR13476(B)] (ab181839), expandable thumbnail](https://content.abcam.com/products/images/gclc-antibody-epr13476b-ab181839--western-blot-img85831.jpg "Western blot - Anti-GCLC antibody [EPR13476(B)] (ab181839)")
Western blot - Anti-GCLC antibody [EPR13476(B)] (ab181839)
All lanes: Western blot - Anti-GCLC antibody [EPR13476(B)] (ab181839) at 1/5000 dilution
All lanes: HepG2 cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 73 kDa
Observed band size: 73 kDa
![Western blot - Anti-GCLC antibody [EPR13476(B)] (ab181839), expandable thumbnail](https://content.abcam.com/products/images/gclc-antibody-epr13476b-ab181839--western-blot-img124459.jpg "Western blot - Anti-GCLC antibody [EPR13476(B)] (ab181839)")
Western blot - Anti-GCLC antibody [EPR13476(B)] (ab181839)
All lanes: Western blot - Anti-GCLC antibody [EPR13476(B)] (ab181839) at 1/10000 dilution
Lane 1: Human fetal liver lysate at 10 µg
Lane 2: A549 cell lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 73 kDa
Observed band size: 73 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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