AB181839

Anti-GCLC antibody [EPR13476(B)]

Name: Anti-GCLC antibody [EPR13476(B)] (ab181839) | Abcam
Brand: Abcam Limited
SKU: AB181839
Price: 380 USD
Availability: InStock
Rating: 3 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(3 Publications)

Rabbit Recombinant Monoclonal GCLC antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications.

View Alternative Names

GLCL, GLCLC, GCLC, Glutamate--cysteine ligase catalytic subunit, GCS heavy chain, Gamma-ECS, Gamma-glutamylcysteine synthetase

2 Images

Supplier Data

Western blot - Anti-GCLC antibody [EPR13476(B)] (AB181839)

All lanes:

Western blot - Anti-GCLC antibody [EPR13476(B)] (ab181839) at 1/5000 dilution

All lanes:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 73 kDa

Observed band size: 73 kDa

false

Supplier Data

Western blot - Anti-GCLC antibody [EPR13476(B)] (AB181839)

All lanes:

Western blot - Anti-GCLC antibody [EPR13476(B)] (ab181839) at 1/10000 dilution

Lane 1:

Human fetal liver lysate at 10 µg

Lane 2:

A549 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 73 kDa

Observed band size: 73 kDa

false

Carrier free

Anti-GCLC antibody [EPR13476(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13476(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the ATP-dependent ligation of L-glutamate and L-cysteine and participates in the first and rate-limiting step in glutathione biosynthesis.

See full target information GCLC

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Microbial cell (Graz, Austria) 10:157-169 PubMed37545643

2023

Caspase 3 exhibits a yeast metacaspase proteostasis function that protects mitochondria from toxic TDP43 aggregates.

Applications

Unspecified application

Species

Unspecified reactive species

Steve Brunette,Anupam Sharma,Ryan Bell,Lawrence Puente,Lynn A Megeney

Neurochemical research 48:2542-2551 PubMed37061657

2023

Dithiolethiones D3T and ACDT Protect Against Iron Overload-Induced Cytotoxicity and Serve as Ferroptosis Inhibitors in U-87 MG Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Neha Kulkarni,Rajitha Gadde,Swati Betharia

American journal of cancer research 11:2911-2927 PubMed34249435

2021

Glutamate-cysteine ligase catalytic subunit as a therapeutic target in acute myeloid leukemia and solid tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Chiou-Hong Lin,John P Vu,Chen-Yen Yang,Mint Sirisawad,Chun-Te Chen,Hung Dao,Jing Liu,Xuan Ma,Chin Pan,Joseph Cefalu,Chris Tse,Erica Jackson,Hsu-Ping Kuo

View all publications

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Anti-GCLC antibody [EPR13476(B)]

Western blot - Anti-GCLC antibody [EPR13476(B)] (AB181839)

Western blot - Anti-GCLC antibody [EPR13476(B)] (AB181839)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (3)

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