Rabbit Polyclonal GCLM antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GCLM aa 1 to C-terminus.
Images
Publications
Filter by
- American journal of translational research 13:11178-111932021Bladder cancer cells prevent cisplatin-induced oxidative stress by upregulating Nestin1 expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYifan Zhang,Yunlong LiPubMed 34786050
- Cellular and molecular gastroenterology and hepatology :2021p53-independent induction of p21 fails to control regeneration and hepatocarcinogenesis in a murine liver injury model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaura Elisa Buitrago-Molina,Silke Marhenke,Diana Becker,Robert Geffers,Timo Itzel,Andreas Teufel,Hartmut Jaeschke,André Lechel,Kristian Unger,Jovana Markovic,Amar Deep Sharma,Jens U Marquardt,Michael Saborowski,Anna Saborowski,Arndt VogelPubMed 33484913
- Mechanisms of ageing and development 180:70-812019Astragaloside IV and ferulic acid synergistically promote neurite outgrowth through Nrf2 activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYini Liang,Yu Zou,Chengu Niu,Yingcai NiuPubMed 30978363
- Molecular metabolism 23:1-132019Dysregulated transmethylation leading to hepatocellular carcinoma compromises redox homeostasis and glucose formation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCurtis C Hughey et. al.PubMed 30850319
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human GCLM aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P48507
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
| Rat | Tested | Expected |
| Cow | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Rat | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Alternative names
GLCLR, GCLM, Glutamate--cysteine ligase regulatory subunit, GCS light chain, Gamma-ECS regulatory subunit, Gamma-glutamylcysteine synthetase regulatory subunit, Glutamate--cysteine ligase modifier subunit
Recommended products
Rabbit Polyclonal GCLM antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GCLM aa 1 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human GCLM aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P48507
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Western blot - Anti-GCLM antibody (ab153967)
12% SDS PAGE
All lanes: Western blot - Anti-GCLM antibody (ab153967) at 1/1000 dilution
All lanes: A431 whole cell lysate at 30 µg
Predicted band size: 31 kDa
Western blot - Anti-GCLM antibody (ab153967)
12% SDS PAGE
All lanes: Western blot - Anti-GCLM antibody (ab153967) at 1/1000 dilution
All lanes: C8D30 whole cell lysate at 30 µg
Predicted band size: 31 kDa
Western blot - Anti-GCLM antibody (ab153967)
12% SDS PAGE
All lanes: Western blot - Anti-GCLM antibody (ab153967) at 1/1000 dilution
All lanes: Rat liver lysate at 50 µg
Predicted band size: 31 kDa
Immunocytochemistry/ Immunofluorescence - Anti-GCLM antibody (ab153967)
Immunofluorescent analysis of methanol-fixed HeLa cells labeling GCLM with ab153967 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com