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AB153967

Anti-GCLM antibody

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(5 Publications)

Rabbit Polyclonal GCLM antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GCLM aa 1 to C-terminus.

View Alternative Names

GLCLR, GCLM, Glutamate--cysteine ligase regulatory subunit, GCS light chain, Gamma-ECS regulatory subunit, Gamma-glutamylcysteine synthetase regulatory subunit, Glutamate--cysteine ligase modifier subunit

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-GCLM antibody (AB153967)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GCLM antibody (AB153967)

Immunofluorescent analysis of methanol-fixed HeLa cells labeling GCLM with ab153967 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.

Western blot - Anti-GCLM antibody (AB153967)
  • WB

Supplier Data

Western blot - Anti-GCLM antibody (AB153967)

12% SDS PAGE

All lanes:

Western blot - Anti-GCLM antibody (ab153967) at 1/1000 dilution

All lanes:

A431 whole cell lysate at 30 µg

Predicted band size: 31 kDa

false

Western blot - Anti-GCLM antibody (AB153967)
  • WB

Supplier Data

Western blot - Anti-GCLM antibody (AB153967)

12% SDS PAGE

All lanes:

Western blot - Anti-GCLM antibody (ab153967) at 1/1000 dilution

All lanes:

C8D30 whole cell lysate at 30 µg

Predicted band size: 31 kDa

false

Western blot - Anti-GCLM antibody (AB153967)
  • WB

Supplier Data

Western blot - Anti-GCLM antibody (AB153967)

12% SDS PAGE

All lanes:

Western blot - Anti-GCLM antibody (ab153967) at 1/1000 dilution

All lanes:

Rat liver lysate at 50 µg

Predicted band size: 31 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human GCLM aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P48507

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information GCLM
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Antioxidants (Basel, Switzerland) 13: PubMed38397767

2024

Taurine Chloramine-Mediated Nrf2 Activation and HO-1 Induction Confer Protective Effects in Astrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Song-I Seol,In Soon Kang,Ji Seok Lee,Ja-Kyeong Lee,Chaekyun Kim

American journal of translational research 13:11178-11193 PubMed34786050

2021

Bladder cancer cells prevent cisplatin-induced oxidative stress by upregulating Nestin1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Yifan Zhang,Yunlong Li

Cellular and molecular gastroenterology and hepatology : PubMed33484913

2021

p53-independent induction of p21 fails to control regeneration and hepatocarcinogenesis in a murine liver injury model.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Elisa Buitrago-Molina,Silke Marhenke,Diana Becker,Robert Geffers,Timo Itzel,Andreas Teufel,Hartmut Jaeschke,André Lechel,Kristian Unger,Jovana Markovic,Amar Deep Sharma,Jens U Marquardt,Michael Saborowski,Anna Saborowski,Arndt Vogel

Mechanisms of ageing and development 180:70-81 PubMed30978363

2019

Astragaloside IV and ferulic acid synergistically promote neurite outgrowth through Nrf2 activation.

Applications

Unspecified application

Species

Unspecified reactive species

Yini Liang,Yu Zou,Chengu Niu,Yingcai Niu

Molecular metabolism 23:1-13 PubMed30850319

2019

Dysregulated transmethylation leading to hepatocellular carcinoma compromises redox homeostasis and glucose formation.

Applications

Unspecified application

Species

Unspecified reactive species

Curtis C Hughey,Freyja D James,Zhizhang Wang,Mickael Goelzer,David H Wasserman
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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