Rabbit Recombinant Monoclonal GCLM antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Mouse, Rat, Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCLM antibody [EPR6667] - BSA and Azide free (AB248137), expandable thumbnail](https://content.abcam.com/products/images/gclm-antibody-epr6667-bsa-and-azide-free-ab248137--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134263.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCLM antibody [EPR6667] - BSA and Azide free (AB248137)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCLM antibody [EPR6667] - BSA and Azide free (AB248137), expandable thumbnail](https://content.abcam.com/products/images/gclm-antibody-epr6667-bsa-and-azide-free-ab248137--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img114160.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCLM antibody [EPR6667] - BSA and Azide free (AB248137)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCLM antibody [EPR6667] - BSA and Azide free (AB248137), expandable thumbnail](https://content.abcam.com/products/images/gclm-antibody-epr6667-bsa-and-azide-free-ab248137--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img55865.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCLM antibody [EPR6667] - BSA and Azide free (AB248137)")
![Western blot - Anti-GCLM antibody [EPR6667] - BSA and Azide free (AB248137), expandable thumbnail](https://content.abcam.com/products/images/gclm-antibody-epr6667-bsa-and-azide-free-ab248137--western-blot-img53601.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GCLM antibody [EPR6667] - BSA and Azide free (AB248137)")
![Western blot - Anti-GCLM antibody [EPR6667] - BSA and Azide free (AB248137), expandable thumbnail](https://content.abcam.com/products/images/gclm-antibody-epr6667-bsa-and-azide-free-ab248137--western-blot-img2151.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GCLM antibody [EPR6667] - BSA and Azide free (AB248137)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | |
|---|---|---|---|---|
| Human | Expected | Not recommended | Tested | Tested |
| Mouse | Expected | Not recommended | Expected | Tested |
| Rat | Expected | Not recommended | Expected | Tested |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target data
Alternative names
GLCLR, GCLM, Glutamate--cysteine ligase regulatory subunit, GCS light chain, Gamma-ECS regulatory subunit, Gamma-glutamylcysteine synthetase regulatory subunit, Glutamate--cysteine ligase modifier subunit
Recommended products
Rabbit Recombinant Monoclonal GCLM antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Mouse, Rat, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR6667
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab248137 is the carrier-free version of Anti-GCLM antibody [EPR6667] ab126704.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCLM antibody [EPR6667] - BSA and Azide free (ab248137), expandable thumbnail](https://content.abcam.com/products/images/gclm-antibody-epr6667-bsa-and-azide-free-ab248137--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134263.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCLM antibody [EPR6667] - BSA and Azide free (ab248137)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCLM antibody [EPR6667] - BSA and Azide free (ab248137)
This data was developed using Anti-GCLM antibody [EPR6667] ab126704, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat liver tissue sections labeling GCLM with purified Anti-GCLM antibody [EPR6667] ab126704 at 1/50 dilution (2.4 µg/mL). Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCLM antibody [EPR6667] - BSA and Azide free (ab248137), expandable thumbnail](https://content.abcam.com/products/images/gclm-antibody-epr6667-bsa-and-azide-free-ab248137--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img114160.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCLM antibody [EPR6667] - BSA and Azide free (ab248137)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCLM antibody [EPR6667] - BSA and Azide free (ab248137)
This data was developed using Anti-GCLM antibody [EPR6667] ab126704, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cardiac muscle tissue sections labeling GCLM with purified Anti-GCLM antibody [EPR6667] ab126704 at 1/50 dilution (2.4 µg/mL). Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCLM antibody [EPR6667] - BSA and Azide free (ab248137), expandable thumbnail](https://content.abcam.com/products/images/gclm-antibody-epr6667-bsa-and-azide-free-ab248137--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img55865.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCLM antibody [EPR6667] - BSA and Azide free (ab248137)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCLM antibody [EPR6667] - BSA and Azide free (ab248137)
This data was developed using Anti-GCLM antibody [EPR6667] ab126704, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human bladder cancer tissue sections labeling GCLM with purified Anti-GCLM antibody [EPR6667] ab126704 at 1/50 dilution (2.4 µg/mL). Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.![Western blot - Anti-GCLM antibody [EPR6667] - BSA and Azide free (ab248137), expandable thumbnail](https://content.abcam.com/products/images/gclm-antibody-epr6667-bsa-and-azide-free-ab248137--western-blot-img53601.jpg "Western blot - Anti-GCLM antibody [EPR6667] - BSA and Azide free (ab248137)")
Western blot - Anti-GCLM antibody [EPR6667] - BSA and Azide free (ab248137)
This data was developed using Anti-GCLM antibody [EPR6667] ab126704, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-GCLM antibody [EPR6667] (Anti-GCLM antibody [EPR6667] ab126704) at 1/10000 dilution
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lane 3: PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysate
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 31 kDa
![Western blot - Anti-GCLM antibody [EPR6667] - BSA and Azide free (ab248137), expandable thumbnail](https://content.abcam.com/products/images/gclm-antibody-epr6667-bsa-and-azide-free-ab248137--western-blot-img2151.jpg "Western blot - Anti-GCLM antibody [EPR6667] - BSA and Azide free (ab248137)")
Western blot - Anti-GCLM antibody [EPR6667] - BSA and Azide free (ab248137)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-GCLM antibody [EPR6667] ab126704).
Lanes 1 - 3: Merged signal (red and green). Green - Anti-GCLM antibody [EPR6667] ab126704 observed at 31 kDa. Red - loading control, Anti-Vinculin antibody [VIN-54] ab130007, observed at 130 kDa.
Anti-GCLM antibody [EPR6667] ab126704 was shown to recognize GCLM in wild-type HAP1 cells as signal was lost at the expected MW in GCLM knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and GCLM knockout samples were subjected to SDS-PAGE. Anti-GCLM antibody [EPR6667] ab126704 and Anti-Vinculin antibody [VIN-54] ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.All lanes: Western blot - Anti-GCLM antibody [EPR6667] (Anti-GCLM antibody [EPR6667] ab126704) at 1 µg/mL
Lane 1: Wild-type HAP1 whole cell lysate at 20 µg
Lane 2: GCLM knockout HAP1 whole cell lysate at 20 µg
Lane 3: HeLa whole cell lysate at 20 µg
Predicted band size: 31 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com