AB86139

Anti-GCN1 antibody

Name: Anti-GCN1 antibody. Rabbit polyclonal (ab86139) | Abcam
Brand: Abcam Limited
SKU: AB86139
Price: 420 USD
Availability: InStock
Rating: 4 (2 reviews)

(2 Reviews)

(6 Publications)

Rabbit Polyclonal GCN1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human GCN1.

View Alternative Names

GCN1L1, KIAA0219, GCN1, Stalled ribosome sensor GCN1, GCN1 eIF-2-alpha kinase activator homolog, GCN1-like protein 1, General control of amino-acid synthesis 1-like protein 1, Translational activator GCN1, HsGCN1

2 Images

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Immunoprecipitation - Anti-GCN1 antibody (AB86139)

Detection of Human GCN1 by Immunoprecipitation, using ab86139 at 3μg/mg lysate. Image shows immunoprecipitated GCN1 detected with post IP WB, loading 20% of IP and using HeLa whole cell lysate at 1mg, with ab86139 at 1 μg/ml in lane 1 and control IgG in lane 2. Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-GCN1 antibody (ab86139)

Predicted band size: 293 kDa

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Western blot - Anti-GCN1 antibody (AB86139)

All lanes:

Western blot - Anti-GCN1 antibody (ab86139) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 293 kDa

Observed band size: 293 kDa

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Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human GCN1. The exact immunogen used to generate this antibody is proprietary information.

Q92616

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Bat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Elephant": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ribosome collision sensor that plays a key role in the RNF14-RNF25 translation quality control pathway, a pathway that takes place when a ribosome has stalled during translation, and which promotes ubiquitination and degradation of translation factors on stalled ribosomes (PubMed : 32610081, PubMed : 36638793, PubMed : 37651229, PubMed : 37951215, PubMed : 37951216). Directly binds to the ribosome and acts as a sentinel for colliding ribosomes : activated following ribosome stalling and promotes recruitment of RNF14, which directly ubiquitinates EEF1A1/eEF1A, leading to its degradation (PubMed : 36638793, PubMed : 37951215, PubMed : 37951216). In addition to EEF1A1/eEF1A, the RNF14-RNF25 translation quality control pathway mediates degradation of ETF1/eRF1 and ubiquitination of ribosomal protein (PubMed : 36638793, PubMed : 37651229). GCN1 also acts as a positive activator of the integrated stress response (ISR) by mediating activation of EIF2AK4/GCN2 in response to amino acid starvation (By similarity). Interaction with EIF2AK4/GCN2 on translating ribosomes stimulates EIF2AK4/GCN2 kinase activity, leading to phosphorylation of eukaryotic translation initiation factor 2 (eIF-2-alpha/EIF2S1) (By similarity). EIF2S1/eIF-2-alpha phosphorylation converts EIF2S1/eIF-2-alpha into a global protein synthesis inhibitor, leading to a global attenuation of cap-dependent translation, and thus to a reduced overall utilization of amino acids, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion (By similarity).

See full target information GCN1

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Molecular cell 83:4290-4303.e9 PubMed37951216

2023

RNF14-dependent atypical ubiquitylation promotes translation-coupled resolution of RNA-protein crosslinks.

Applications

Unspecified application

Species

Unspecified reactive species

Shubo Zhao,Jacqueline Cordes,Karolina M Caban,Maximilian J Götz,Timur Mackens-Kiani,Anthony J Veltri,Niladri K Sinha,Pedro Weickert,Selay Kaya,Graeme Hewitt,Danny D Nedialkova,Thomas Fröhlich,Roland Beckmann,Allen R Buskirk,Rachel Green,Julian Stingele

Parasites & vectors 15:383 PubMed36271393

2022

General control nonderepressible 1 interacts with cationic amino acid transporter 1 and affects Aedes aegypti fecundity.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew Pinch,Theodore Muka,Yashoda Kandel,Mahesh Lamsal,Nathan Martinez,Marialuisa Teixeira,Dmitri Y Boudko,Immo A Hansen

International journal of molecular sciences 23: PubMed35328622

2022

Inducible Systemic Deletion in Mice Leads to Transient Body Weight Loss upon Tamoxifen Treatment Associated with Decrease of Fat and Liver Glycogen Storage.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Liu,Shuya Kasai,Yota Tatara,Hiromi Yamazaki,Junsei Mimura,Seiya Mizuno,Fumihiro Sugiyama,Satoru Takahashi,Tsubasa Sato,Taku Ozaki,Kunikazu Tanji,Koichi Wakabayashi,Hayato Maeda,Hiroki Mizukami,Yasuhiro Shinkai,Yoshito Kumagai,Hirofumi Tomita,Ken Itoh

Molecular cancer research : MCR 20:607-621 PubMed34992146

2022

MIRO2 Regulates Prostate Cancer Cell Growth via GCN1-Dependent Stress Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Madison Furnish,Dillon P Boulton,Victoria Genther,Denisa Grofova,Mitchell Lee Ellinwood,Lina Romero,M Scott Lucia,Scott D Cramer,M Cecilia Caino

Neuron 93:1082-1093.e5 PubMed28279353

2017

Small-Molecule Stabilization of 14-3-3 Protein-Protein Interactions Stimulates Axon Regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew Kaplan,Barbara Morquette,Antje Kroner,SooYuen Leong,Carolin Madwar,Ricardo Sanz,Sara L Banerjee,Jack Antel,Nicolas Bisson,Samuel David,Alyson E Fournier

Molecular & cellular proteomics : MCP 15:1220-31 PubMed26711467

2015

Application of Mass Spectrometry Profiling to Establish Brusatol as an Inhibitor of Global Protein Synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Steffan Vartanian,Taylur P Ma,James Lee,Peter M Haverty,Donald S Kirkpatrick,Kebing Yu,David Stokoe

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Anti-GCN1 antibody

Immunoprecipitation - Anti-GCN1 antibody (AB86139)

Western blot - Anti-GCN1 antibody (AB86139)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (6)

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Product promise