Anti-GCN1 antibody [EPR28542-86] - BSA and Azide free

• WB

Supplier Data

Western blot - Anti-GCN1 antibody [EPR28542-86] - BSA and Azide free (AB315473)

This data was developed using ab315472, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Lysates were freshly made and used immediately to minimize protein degradation.

Exposure time : Lane1, 4 : 180 seconds, lane2-3 : 59 seconds

All lanes:

Western blot - Anti-GCN1 antibody [EPR28542-86] (<a href='/en-us/products/primary-antibodies/gcn1-antibody-epr28542-86-ab315472'>ab315472</a>) at 1/1000 dilution

Lane 1:

Human skeletal muscle tissue at 20 µg

Lane 2:

PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

DU-145 (human prostate carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 295 kDa

false

• WB

Supplier Data

Western blot - Anti-GCN1 antibody [EPR28542-86] - BSA and Azide free (AB315473)

This data was developed using ab315472, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, ab315472 was shown to bind specifically to GCN1.

Target of interest was observed at 295 KD in wild-type HEK293T cell lysates (lane 1, wild-type cell line ab255449) with no signal observed at this size in GCN1L1 knockout cell line (lane 2, knockout cell line ab266780/ knockout cell lysate ab263213).

Lysates were freshly made and used immediately to minimize protein degradation.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-GCN1 antibody [EPR28542-86] (<a href='/en-us/products/primary-antibodies/gcn1-antibody-epr28542-86-ab315472'>ab315472</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 2:

GCN1L1 knockout HEK293T whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 295 kDa,124 kDa

false

Exposure time: 48s

• WB

Supplier Data

Western blot - Anti-GCN1 antibody [EPR28542-86] - BSA and Azide free (AB315473)

This data was developed using ab315472, the same antibody clone in a different buffer formulation.

Lysates were freshly made and used immediately to minimize protein degradation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-GCN1 antibody [EPR28542-86] (<a href='/en-us/products/primary-antibodies/gcn1-antibody-epr28542-86-ab315472'>ab315472</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse liver tissue lysate at 20 µg

Lane 3:

Rat liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 295 kDa

false

Exposure time: 180s