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Rabbit Recombinant Monoclonal GCN2 antibody. Suitable for WB and reacts with Rat, Mouse, Human samples.

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Images

Western blot - Anti-GCN2 antibody [EPR25345-125] (AB302609), expandable thumbnail
  • Western blot - Anti-GCN2 antibody [EPR25345-125] (AB302609), expandable thumbnail
  • Western blot - Anti-GCN2 antibody [EPR25345-125] (AB302609), expandable thumbnail
  • Western blot - Anti-GCN2 antibody [EPR25345-125] (AB302609), expandable thumbnail
  • Western blot - Anti-GCN2 antibody [EPR25345-125] (AB302609), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow Cyt (Intra)IHC-PWBICC/IF
Human
Not recommended
Not recommended
Not recommended
Tested
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Tested
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Tested
Not recommended

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Rat, Mouse, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Rat
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) in response to low amino acid availability (PubMed:25329545, PubMed:32610081). Plays a role as an activator of the integrated stress response (ISR) required for adaptation to amino acid starvation (By similarity). EIF2S1/eIF-2-alpha phosphorylation in response to stress converts EIF2S1/eIF-2-alpha into a global protein synthesis inhibitor, leading to a global attenuation of cap-dependent translation, and thus to a reduced overall utilization of amino acids, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion (PubMed:32610081). Binds uncharged tRNAs (By similarity). Required for the translational induction of protein kinase PRKCH following amino acid starvation (By similarity). Involved in cell cycle arrest by promoting cyclin D1 mRNA translation repression after the unfolded protein response pathway (UPR) activation or cell cycle inhibitor CDKN1A/p21 mRNA translation activation in response to amino acid deprivation (PubMed:26102367). Plays a role in the consolidation of synaptic plasticity, learning as well as formation of long-term memory (By similarity). Plays a role in neurite outgrowth inhibition (By similarity). Plays a proapoptotic role in response to glucose deprivation (By similarity). Promotes global cellular protein synthesis repression in response to UV irradiation independently of the stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK) and p38 MAPK signaling pathways (By similarity). Plays a role in the antiviral response against alphavirus infection; impairs early viral mRNA translation of the incoming genomic virus RNA, thus preventing alphavirus replication (By similarity). (Microbial infection) Plays a role in modulating the adaptive immune response to yellow fever virus infection; promotes dendritic cells to initiate autophagy and antigene presentation to both CD4(+) and CD8(+) T-cells under amino acid starvation (PubMed:24310610).

Alternative names

GCN2, KIAA1338, EIF2AK4, eIF-2-alpha kinase GCN2, Eukaryotic translation initiation factor 2-alpha kinase 4, GCN2-like protein

Recommended products

Rabbit Recombinant Monoclonal GCN2 antibody. Suitable for WB and reacts with Rat, Mouse, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR25345-125
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-GCN2 antibody [EPR25345-125] (ab302609), expandable thumbnail

    Western blot - Anti-GCN2 antibody [EPR25345-125] (ab302609)

    GCN2 Western blot staining using rabbit Anti-GCN2 antibody

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/1000000 dilution.

    Lysate was freshly made and used for Western blotting immediately to minimize protein degradation.

    All lanes: Western blot - Anti-GCN2 antibody [EPR25345-125] (ab302609) at 1/1000 dilution

    Lane 1: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

    Lane 2: Fresh HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Performed under non-reducing conditions.

    Observed band size: 187 kDa

    Exposure time: 60s

  • Western blot - Anti-GCN2 antibody [EPR25345-125] (ab302609), expandable thumbnail

    Western blot - Anti-GCN2 antibody [EPR25345-125] (ab302609)

    GCN2 Western blot staining using rabbit Anti-GCN2 antibody

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/1000000 dilution.

    Anti-GCN2 antibody [EPR5970(2)] ab134053 is more sensitive than ab302609 in WB testing.

    Lanes 1 - 2: Western blot - Anti-GCN2 antibody [EPR25345-125] (ab302609) at 1/1000 dilution

    Lanes 3 - 4: Western blot - Anti-GCN2 antibody [EPR5970(2)] (Anti-GCN2 antibody [EPR5970(2)] ab134053) at 1/1000 dilution

    Lanes 1 and 3: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

    Lanes 2 and 4: 293T (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 187 kDa

    Exposure time: 60s

  • Western blot - Anti-GCN2 antibody [EPR25345-125] (ab302609), expandable thumbnail

    Western blot - Anti-GCN2 antibody [EPR25345-125] (ab302609)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    All lanes: Western blot - Anti-GCN2 antibody [EPR25345-125] (ab302609) at 1/1000 dilution

    Lane 1: MEF (mouse embryonic fibroblast (immortalized)), whole cell lysate at 20 µg

    Lane 2: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg

    Lane 3: Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate at 20 µg

    Lane 4: MOLT-4 (human lymphoblastic leukemia T lymphoblast), whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Observed band size: 186 kDa

    Exposure time: 3min

  • Western blot - Anti-GCN2 antibody [EPR25345-125] (ab302609), expandable thumbnail

    Western blot - Anti-GCN2 antibody [EPR25345-125] (ab302609)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    All lanes: Western blot - Anti-GCN2 antibody [EPR25345-125] (ab302609) at 1/1000 dilution

    Lane 1: Wild-type HAP1 (human chronic myelogenous leukemia near-haploid cell line), whole cell lysate

    Lane 2: EIF2AK4 (GCN2) KO HAP1 whole cell lysate

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Observed band size: 186 kDa

    Exposure time: 3min

  • Western blot - Anti-GCN2 antibody [EPR25345-125] (ab302609), expandable thumbnail

    Western blot - Anti-GCN2 antibody [EPR25345-125] (ab302609)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST


    Exposure time: 3 minutes

    All lanes: Western blot - Anti-GCN2 antibody [EPR25345-125] (ab302609) at 1/1000 dilution

    All lanes: C6 (rat glial tumor glial cell), whole cell lysate 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 186 kDa

    Exposure time: 3min

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com