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Rabbit Recombinant Monoclonal GCN2 antibody. Carrier free. Suitable for WB and reacts with Rat, Mouse, Human samples.

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Images

Western blot - Anti-GCN2 antibody [EPR25345-125] (BSA and Azide free) (AB302610), expandable thumbnail
  • Western blot - Anti-GCN2 antibody [EPR25345-125] (BSA and Azide free) (AB302610), expandable thumbnail
  • Western blot - Anti-GCN2 antibody [EPR25345-125] (BSA and Azide free) (AB302610), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow Cyt (Intra)IHC-PWBICC/IF
Human
Not recommended
Not recommended
Not recommended
Tested
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Tested
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Tested
Not recommended

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Human, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Rat, Mouse, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Rat, Mouse, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Target data

Function

Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) in response to low amino acid availability (PubMed:25329545). Plays a role as an activator of the integrated stress response (ISR) required for adaptation to amino acid starvation (By similarity). EIF2S1/eIF-2-alpha phosphorylation in response to stress converts EIF2S1/eIF-2-alpha in a global protein synthesis inhibitor, leading to a global attenuation of cap-dependent translation, and thus to a reduced overall utilization of amino acids, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion (By similarity). Binds uncharged tRNAs (By similarity). Involved in cell cycle arrest by promoting cyclin D1 mRNA translation repression after the unfolded protein response pathway (UPR) activation or cell cycle inhibitor CDKN1A/p21 mRNA translation activation in response to amino acid deprivation (PubMed:26102367). Plays a role in the consolidation of synaptic plasticity, learning as well as formation of long-term memory (By similarity). Plays a role in neurite outgrowth inhibition (By similarity). Plays a proapoptotic role in response to glucose deprivation (By similarity). Promotes global cellular protein synthesis repression in response to UV irradiation independently of the stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK) and p38 MAPK signaling pathways (By similarity). Plays a role in the antiviral response against alphavirus infection; impairs early viral mRNA translation of the incoming genomic virus RNA, thus preventing alphavirus replication (By similarity).(Microbial infection) Plays a role in modulating the adaptive immune response to yellow fever virus infection; promotes dendritic cells to initiate autophagy and antigene presentation to both CD4(+) and CD8(+) T-cells under amino acid starvation (PubMed:24310610).

Alternative names

eIF-2-alpha kinase GCN2, Eukaryotic translation initiation factor 2-alpha kinase 4, GCN2-like protein, GCN2, KIAA1338, EIF2AK4

Recommended products

Rabbit Recombinant Monoclonal GCN2 antibody. Carrier free. Suitable for WB and reacts with Rat, Mouse, Human samples.

Alternative names

eIF-2-alpha kinase GCN2, Eukaryotic translation initiation factor 2-alpha kinase 4, GCN2-like protein, GCN2, KIAA1338, EIF2AK4

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR25345-125

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-GCN2 antibody [EPR25345-125] (BSA and Azide free) (ab302610), expandable thumbnail

    Western blot - Anti-GCN2 antibody [EPR25345-125] (BSA and Azide free) (ab302610)

    This data was developed using Anti-GCN2 antibody [EPR25345-125] ab302609, the same antibody clone in a different buffer
    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    This blot was developed using a high sensitivity ECL substrate.

    Exposure time: 3 minutes

    All lanes: Western blot - Anti-GCN2 antibody [EPR25345-125] (Anti-GCN2 antibody [EPR25345-125] ab302609) at 1/1000 dilution

    Lane 1: Wild-type HAP1 (human chronic myelogenous leukemia near-haploid cell line), whole cell lysate

    Lane 2: EIF2AK4 (GCN2) KO HAP1 whole cell lysate

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 186 kDa

    Exposure time: 3min

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    This blot was developed using a high sensitivity ECL substrate.

    Exposure time: 3 minutes

  • Western blot - Anti-GCN2 antibody [EPR25345-125] (BSA and Azide free) (ab302610), expandable thumbnail

    Western blot - Anti-GCN2 antibody [EPR25345-125] (BSA and Azide free) (ab302610)

    This data was developed using Anti-GCN2 antibody [EPR25345-125] ab302609, the same antibody clone in a different buffer
    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    This blot was developed using a high sensitivity ECL substrate.

    Exposure time: 3 minutes

    All lanes: Western blot - Anti-GCN2 antibody [EPR25345-125] (Anti-GCN2 antibody [EPR25345-125] ab302609) at 1/1000 dilution

    Lane 1: MEF (mouse embryonic fibroblast (immortalized)), whole cell lysate 20 µg

    Lane 2: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate 20 µg

    Lane 3: Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate 20 µg

    Lane 4: MOLT-4 (human lymphoblastic leukemia T lymphoblast), whole cell lysate 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 186 kDa

    Exposure time: 3min

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    This blot was developed using a high sensitivity ECL substrate.

    Exposure time: 3 minutes

  • Western blot - Anti-GCN2 antibody [EPR25345-125] (BSA and Azide free) (ab302610), expandable thumbnail

    Western blot - Anti-GCN2 antibody [EPR25345-125] (BSA and Azide free) (ab302610)

    This data was developed using Anti-GCN2 antibody [EPR25345-125] ab302609, the same antibody clone in a different buffer
    Blocking and diluting buffer and concentration: 5% NFDM/TBST


    Exposure time: 3 minutes

    All lanes: Western blot - Anti-GCN2 antibody [EPR25345-125] (Anti-GCN2 antibody [EPR25345-125] ab302609) at 1/1000 dilution

    All lanes: C6 (rat glial tumor glial cell), whole cell lysate 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 186 kDa

    Exposure time: 3min

    Blocking and diluting buffer and concentration: 5% NFDM/TBST


    Exposure time: 3 minutes

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com