Rabbit Recombinant Monoclonal GCN2 antibody. Carrier free. Suitable for WB and reacts with Rat, Mouse, Human samples.
Images
![Western blot - Anti-GCN2 antibody [EPR25345-125] (BSA and Azide free) (AB302610), expandable thumbnail](https://content.abcam.com/products/images/gcn2-antibody-epr25345-125-bsa-and-azide-free-ab302610--western-blot-img446597.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GCN2 antibody [EPR25345-125] (BSA and Azide free) (AB302610)")
![Western blot - Anti-GCN2 antibody [EPR25345-125] (BSA and Azide free) (AB302610), expandable thumbnail](https://content.abcam.com/products/images/gcn2-antibody-epr25345-125-bsa-and-azide-free-ab302610--western-blot-img446596.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GCN2 antibody [EPR25345-125] (BSA and Azide free) (AB302610)")
![Western blot - Anti-GCN2 antibody [EPR25345-125] (BSA and Azide free) (AB302610), expandable thumbnail](https://content.abcam.com/products/images/gcn2-antibody-epr25345-125-bsa-and-azide-free-ab302610--western-blot-img401135.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GCN2 antibody [EPR25345-125] (BSA and Azide free) (AB302610)")
![Western blot - Anti-GCN2 antibody [EPR25345-125] (BSA and Azide free) (AB302610), expandable thumbnail](https://content.abcam.com/products/images/gcn2-antibody-epr25345-125-bsa-and-azide-free-ab302610--western-blot-img401134.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GCN2 antibody [EPR25345-125] (BSA and Azide free) (AB302610)")
![Western blot - Anti-GCN2 antibody [EPR25345-125] (BSA and Azide free) (AB302610), expandable thumbnail](https://content.abcam.com/products/images/gcn2-antibody-epr25345-125-bsa-and-azide-free-ab302610--western-blot-img401136.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GCN2 antibody [EPR25345-125] (BSA and Azide free) (AB302610)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt (Intra) | IHC-P | WB | ICC/IF | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) in response to low amino acid availability (PubMed:25329545, PubMed:32610081). Plays a role as an activator of the integrated stress response (ISR) required for adaptation to amino acid starvation (By similarity). EIF2S1/eIF-2-alpha phosphorylation in response to stress converts EIF2S1/eIF-2-alpha into a global protein synthesis inhibitor, leading to a global attenuation of cap-dependent translation, and thus to a reduced overall utilization of amino acids, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion (PubMed:32610081). Binds uncharged tRNAs (By similarity). Required for the translational induction of protein kinase PRKCH following amino acid starvation (By similarity). Involved in cell cycle arrest by promoting cyclin D1 mRNA translation repression after the unfolded protein response pathway (UPR) activation or cell cycle inhibitor CDKN1A/p21 mRNA translation activation in response to amino acid deprivation (PubMed:26102367). Plays a role in the consolidation of synaptic plasticity, learning as well as formation of long-term memory (By similarity). Plays a role in neurite outgrowth inhibition (By similarity). Plays a proapoptotic role in response to glucose deprivation (By similarity). Promotes global cellular protein synthesis repression in response to UV irradiation independently of the stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK) and p38 MAPK signaling pathways (By similarity). Plays a role in the antiviral response against alphavirus infection; impairs early viral mRNA translation of the incoming genomic virus RNA, thus preventing alphavirus replication (By similarity). (Microbial infection) Plays a role in modulating the adaptive immune response to yellow fever virus infection; promotes dendritic cells to initiate autophagy and antigene presentation to both CD4(+) and CD8(+) T-cells under amino acid starvation (PubMed:24310610).
Alternative names
GCN2, KIAA1338, EIF2AK4, eIF-2-alpha kinase GCN2, Eukaryotic translation initiation factor 2-alpha kinase 4, GCN2-like protein
Recommended products
Rabbit Recombinant Monoclonal GCN2 antibody. Carrier free. Suitable for WB and reacts with Rat, Mouse, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR25345-125
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-GCN2 antibody [EPR25345-125] (BSA and Azide free) (ab302610), expandable thumbnail](https://content.abcam.com/products/images/gcn2-antibody-epr25345-125-bsa-and-azide-free-ab302610--western-blot-img446597.jpg "Western blot - Anti-GCN2 antibody [EPR25345-125] (BSA and Azide free) (ab302610)")
Western blot - Anti-GCN2 antibody [EPR25345-125] (BSA and Azide free) (ab302610)
GCN2 Western blot staining using rabbit Anti-GCN2 antibody
This data was developed using Anti-GCN2 antibody [EPR25345-125] ab302609, the same antibody clone in a different buffer formulation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/1000000 dilution.
Lysate was freshly made and used for Western blotting immediately to minimize protein degradation.
All lanes: Western blot - Anti-GCN2 antibody [EPR25345-125] (Anti-GCN2 antibody [EPR25345-125] ab302609) at 1/1000 dilution
Lane 1: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 2: Fresh HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Performed under non-reducing conditions.
Observed band size: 187 kDa
Exposure time: 60s
![Western blot - Anti-GCN2 antibody [EPR25345-125] (BSA and Azide free) (ab302610), expandable thumbnail](https://content.abcam.com/products/images/gcn2-antibody-epr25345-125-bsa-and-azide-free-ab302610--western-blot-img446596.jpg "Western blot - Anti-GCN2 antibody [EPR25345-125] (BSA and Azide free) (ab302610)")
Western blot - Anti-GCN2 antibody [EPR25345-125] (BSA and Azide free) (ab302610)
GCN2 Western blot staining using rabbit Anti-GCN2 antibody
This data was developed using Anti-GCN2 antibody [EPR25345-125] ab302609, the same antibody clone in a different buffer formulation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/1000000 dilution.
Anti-GCN2 antibody [EPR5970(2)] ab134053 is more sensitive than Anti-GCN2 antibody [EPR25345-125] ab302609 in WB testing.
Lanes 1 - 2: Western blot - Anti-GCN2 antibody [EPR25345-125] (Anti-GCN2 antibody [EPR25345-125] ab302609) at 1/1000 dilution
Lanes 3 - 4: Western blot - Anti-GCN2 antibody [EPR5970(2)] (Anti-GCN2 antibody [EPR5970(2)] ab134053) at 1/1000 dilution
Lanes 1 and 3: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Lanes 2 and 4: 293T (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 187 kDa
Exposure time: 60s
![Western blot - Anti-GCN2 antibody [EPR25345-125] (BSA and Azide free) (ab302610), expandable thumbnail](https://content.abcam.com/products/images/gcn2-antibody-epr25345-125-bsa-and-azide-free-ab302610--western-blot-img401135.jpg "Western blot - Anti-GCN2 antibody [EPR25345-125] (BSA and Azide free) (ab302610)")
Western blot - Anti-GCN2 antibody [EPR25345-125] (BSA and Azide free) (ab302610)
This data was developed using Anti-GCN2 antibody [EPR25345-125] ab302609, the same antibody clone in a different buffer.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
All lanes: Western blot - Anti-GCN2 antibody [EPR25345-125] (Anti-GCN2 antibody [EPR25345-125] ab302609) at 1/1000 dilution
Lane 1: MEF (mouse embryonic fibroblast (immortalized)), whole cell lysate at 20 µg
Lane 2: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg
Lane 3: Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate at 20 µg
Lane 4: MOLT-4 (human lymphoblastic leukemia T lymphoblast), whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Observed band size: 186 kDa
Exposure time: 3min
![Western blot - Anti-GCN2 antibody [EPR25345-125] (BSA and Azide free) (ab302610), expandable thumbnail](https://content.abcam.com/products/images/gcn2-antibody-epr25345-125-bsa-and-azide-free-ab302610--western-blot-img401134.jpg "Western blot - Anti-GCN2 antibody [EPR25345-125] (BSA and Azide free) (ab302610)")
Western blot - Anti-GCN2 antibody [EPR25345-125] (BSA and Azide free) (ab302610)
This data was developed using Anti-GCN2 antibody [EPR25345-125] ab302609, the same antibody clone in a different buffer.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
All lanes: Western blot - Anti-GCN2 antibody [EPR25345-125] (Anti-GCN2 antibody [EPR25345-125] ab302609) at 1/1000 dilution
Lane 1: Wild-type HAP1 (human chronic myelogenous leukemia near-haploid cell line), whole cell lysate
Lane 2: EIF2AK4 (GCN2) KO HAP1 whole cell lysate
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Developed using the ECL technique.
Observed band size: 186 kDa
Exposure time: 3min
![Western blot - Anti-GCN2 antibody [EPR25345-125] (BSA and Azide free) (ab302610), expandable thumbnail](https://content.abcam.com/products/images/gcn2-antibody-epr25345-125-bsa-and-azide-free-ab302610--western-blot-img401136.jpg "Western blot - Anti-GCN2 antibody [EPR25345-125] (BSA and Azide free) (ab302610)")
Western blot - Anti-GCN2 antibody [EPR25345-125] (BSA and Azide free) (ab302610)
This data was developed using Anti-GCN2 antibody [EPR25345-125] ab302609, the same antibody clone in a different buffer
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 3 minutesAll lanes: Western blot - Anti-GCN2 antibody [EPR25345-125] (Anti-GCN2 antibody [EPR25345-125] ab302609) at 1/1000 dilution
All lanes: C6 (rat glial tumor glial cell), whole cell lysate 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 186 kDa
Exposure time: 3min
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