AB75836

Anti-GCN2 (phospho T899) antibody [EPR2320Y]

Name: Anti-GCN2 (phospho T899) antibody [EPR2320Y] (ab75836) | Abcam
Brand: Abcam Limited
SKU: AB75836
Price: 315 USD
Availability: InStock
Rating: 4 (13 reviews)

RabMAb
Recombinant
20ul selling size
What is this?

(13 Reviews)

(101 Publications)

Anti-GCN2 (phospho T899) antibody [EPR2320Y] (ab75836) is a rabbit monoclonal antibody detecting GCN2 in Western Blot, Dot Blot. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency
- Over 70 publications
- Trusted since 2009

View Alternative Names

GCN2, KIAA1338, EIF2AK4, eIF-2-alpha kinase GCN2, Eukaryotic translation initiation factor 2-alpha kinase 4, GCN2-like protein

5 Images

Lab

Western blot - Anti-GCN2 (phospho T899) antibody [EPR2320Y] (AB75836)

Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-GCN2 (phospho T899) antibody [EPR2320Y] (ab75836) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) grown in serum free media for 24hours, whole cell lysate at 15 µg

Lane 2:

HeLa grown in serum free media for 24hours, then treated with 100nM Calyculin A for 30min, whole cell lysate at 15 µg

Lane 3:

HeLa grown in serum free media for 24hours, then treated with 100nM Calyculin A for 30min, whole cell lysate. Then the membrane was incubated with alkaline phosphatase at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 187 kDa

false

Lab

Western blot - Anti-GCN2 (phospho T899) antibody [EPR2320Y] (AB75836)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-Vinculin antibody [EPR8185] - Loading Control (ab129002) staining at 1/1000,000 dilution.

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

Exposure time : Lane 1 : 20 seconds; Lane 2, 3 : 180 seconds.

Lane 1:

Western blot - Anti-GCN2 (phospho T899) antibody [EPR2320Y] (ab75836) at 1/1000 dilution

Lane 2:

Non-Abcam antibody target pGCN2 at 1/1000 dilution

All lanes:

HeLa (human cervix adenocarcinoma epithelial cell) treated with 50 nM Calyculin A for 2 h. whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 187 kDa

Observed band size: 187 kDa,124 kDa

true

Lab

Western blot - Anti-GCN2 (phospho T899) antibody [EPR2320Y] (AB75836)

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-GCN2 (phospho T899) antibody [EPR2320Y] (ab75836) at 1/1000 dilution

Lane 1:

HeLa cell lysate - untreated at 10 µg

Lane 2:

HeLa cell lysate - treated with Calyculin at 10 µg

Secondary

All lanes:

Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 187 kDa

Observed band size: 220 kDa

false

Lab

Western blot - Anti-GCN2 (phospho T899) antibody [EPR2320Y] (AB75836)

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-GCN2 (phospho T899) antibody [EPR2320Y] (ab75836) at 1/500 dilution

Lane 1:

HeLa cell lysate - untreated at 10 µg

Lane 2:

HeLa cell lysate - treated with Calyculin A at 10 µg

Secondary

All lanes:

Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 187 kDa

Observed band size: 220 kDa

false

Unknown

Dot Blot - Anti-GCN2 (phospho T899) antibody [EPR2320Y] (AB75836)

Dot blot analysis of GCN2 (phospho T899) phospho peptide (Lane 1) and GCN2 non-phospho peptide (Lane 2) labelling GCN2 (phospho T899) phospho peptide with ab75836 at a dilution of 1 : 1000 dilution (0.277μg/ml). A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1 : 20,000 dilution. Blocking buffer : 5% NFDM/TBST. Dilution buffer : 5% NFDM /TBST .

Carrier free

Anti-GCN2 (phospho T899) antibody [EPR2320Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2320Y

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "For unpurified use at 1/500.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Synthetic peptide": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Product Specifications
Anti-GCN2 (phospho T899) antibody [EPR2320Y] (ab75836) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Dot blot, WB in human samples.
Anti-GCN2 (phospho T899) antibody [EPR2320Y] (ab75836) specifically detects GCN2 Phospho-T899 (UniProt ID: Q9P2K8; Molecular weight: 187kDa) and is sold in 100 µL and 1 mL selling sizes.

Quality and Validation
Abcam's high quality manufacturing and validation processes ensure Anti-GCN2 (phospho T899) antibody [EPR2320Y] (ab75836) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-GCN2 (phospho T899) antibody [EPR2320Y] (ab75836) has been cited over 79 times in peer reviewed journals and is trusted by the scientific community.
Anti-GCN2 (phospho T899) antibody [EPR2320Y] (ab75836) has 12 independent reviews from customers.

Related Products
Conjugation-ready, carrier free format available for antibody clone EPR2320Y - ab221935.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) in response to low amino acid availability (PubMed : 25329545, PubMed : 32610081). Plays a role as an activator of the integrated stress response (ISR) required for adaptation to amino acid starvation (By similarity). EIF2S1/eIF-2-alpha phosphorylation in response to stress converts EIF2S1/eIF-2-alpha into a global protein synthesis inhibitor, leading to a global attenuation of cap-dependent translation, and thus to a reduced overall utilization of amino acids, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion (PubMed : 32610081). Binds uncharged tRNAs (By similarity). Required for the translational induction of protein kinase PRKCH following amino acid starvation (By similarity). Involved in cell cycle arrest by promoting cyclin D1 mRNA translation repression after the unfolded protein response pathway (UPR) activation or cell cycle inhibitor CDKN1A/p21 mRNA translation activation in response to amino acid deprivation (PubMed : 26102367). Plays a role in the consolidation of synaptic plasticity, learning as well as formation of long-term memory (By similarity). Plays a role in neurite outgrowth inhibition (By similarity). Plays a proapoptotic role in response to glucose deprivation (By similarity). Promotes global cellular protein synthesis repression in response to UV irradiation independently of the stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK) and p38 MAPK signaling pathways (By similarity). Plays a role in the antiviral response against alphavirus infection; impairs early viral mRNA translation of the incoming genomic virus RNA, thus preventing alphavirus replication (By similarity).. (Microbial infection) Plays a role in modulating the adaptive immune response to yellow fever virus infection; promotes dendritic cells to initiate autophagy and antigene presentation to both CD4(+) and CD8(+) T-cells under amino acid starvation (PubMed : 24310610).

See full target information EIF2AK4 phospho T899

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Publications (101)

Recent publications for all applications. Explore the full list and refine your search

Life science alliance 8: PubMed40912912

2025

Differential cell survival outcomes in response to diverse amino acid stress.

Applications

Unspecified application

Species

Unspecified reactive species

Marion Russier,Alessandra Fiore,Ana Bici,Annette Groß,Maria Tanzer,Assa Yeroslaviz,Matthias Mann,Peter J Murray

Chemical biology & drug design 105:e70147 PubMed40574308

2025

Nesfatin-1 Reduces Cholic Acid-Induced Intrahepatic Cholestasis of Pregnancy by Regulating the General Control Nonderepressible 2/Eukaryotic Initiation Factor 2α Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Liang,Xiaomin Wen,Zhe Sun,Yuqiong Meng,Shuhan Lv

Cell metabolism 37:1715-1731.e11 PubMed40513561

2025

The integrated stress response fine-tunes stem cell fate decisions upon serine deprivation and tissue injury.

Applications

Unspecified application

Species

Unspecified reactive species

Jesse S S Novak,Lisa Polak,Sanjeethan C Baksh,Douglas W Barrows,Marina Schernthanner,Benjamin T Jackson,Elizabeth A N Thompson,Anita Gola,M Deniz Abdusselamoglu,Alain R Bonny,Kevin A U Gonzales,Julia S Brunner,Anna E Bridgeman,Katie S Stewart,Lynette Hidalgo,June Dela Cruz-Racelis,Ji-Dung Luo,Shiri Gur-Cohen,H Amalia Pasolli,Thomas S Carroll,Lydia W S Finley,Elaine Fuchs

Science advances 11:eadt3142 PubMed40138418

2025

The clinical antiprotozoal drug halofuginone promotes weight loss by elevating GDF15 and FGF21.

Applications

Unspecified application

Species

Unspecified reactive species

Suowen Xu,Zhenghong Liu,Tian Tian,Wenqi Zhao,Zhihua Wang,Monan Liu,Mengyun Xu,Fanshun Zhang,Zhidan Zhang,Meijie Chen,Yanjun Yin,Meiming Su,Wenxiang Fang,Wenhao Pan,Shiyong Liu,Min-Dian Li,Peter J Little,Danielle Kamato,Songyang Zhang,Dongdong Wang,Stefan Offermanns,John R Speakman,Jianping Weng

PLoS pathogens 21:e1012934 PubMed39951426

2025

Inhibition of the Integrated stress response by Epstein-Barr virus oncoprotein LMP1 attenuates epithelial cell differentiation and lytic viral reactivation.

Applications

Unspecified application

Species

Unspecified reactive species

Deo R Singh,Yitao Zhang,Sophia J White,Scott E Nelson,Stuart A Fogarty,Abigail S Pawelski,Alisha S Kansra,Shannon C Kenney

Nutrients 16: PubMed39683422

2024

Gut Microbiota Modulates Fgf21 Expression and Metabolic Phenotypes Induced by Ketogenic Diet.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyi Wei,Yunxu Lu,Shangyu Hong

Science signaling 17:eadp1375 PubMed39591412

2024

Coordination between the eIF2 kinase GCN2 and p53 signaling supports purine metabolism and the progression of prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ricardo A Cordova,Noah R Sommers,Andrew S Law,Angela J Klunk,Katherine E Brady,David W Goodrich,Tracy G Anthony,Jeffrey J Brault,Roberto Pili,Ronald C Wek,Kirk A Staschke

Cancer & metabolism 12:31 PubMed39462426

2024

GCN2-SLC7A11 axis coordinates autophagy, cell cycle and apoptosis and regulates cell growth in retinoblastoma upon arginine deprivation.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Wang,Wai Kit Chu,Jason Cheuk Sing Yam,Chi Pui Pang,Yun Chung Leung,Alisa Sau Wun Shum,Sun-On Chan

Cancer research 84:4031-4048 PubMed39240686

2024

MYC Drives mRNA Pseudouridylation to Mitigate Proliferation-Induced Cellular Stress during Cancer Development.

Applications

Unspecified application

Species

Unspecified reactive species

Jane Ding,Mohit Bansal,Yuxia Cao,Bingwei Ye,Rui Mao,Anamika Gupta,Sunil Sudarshan,Han-Fei Ding

The Journal of cell biology 223: PubMed39150520

2024

Integrated stress response activator halofuginone protects mice from diabetes-like phenotypes.

Applications

Unspecified application

Species

Unspecified reactive species

Shashank Rai,Maria Szaruga,Aleksandra P Pitera,Anne Bertolotti

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GCN2 (phospho T899) antibody [EPR2320Y]

Western blot - Anti-GCN2 (phospho T899) antibody [EPR2320Y] (AB75836)

Western blot - Anti-GCN2 (phospho T899) antibody [EPR2320Y] (AB75836)

Western blot - Anti-GCN2 (phospho T899) antibody [EPR2320Y] (AB75836)

Western blot - Anti-GCN2 (phospho T899) antibody [EPR2320Y] (AB75836)

Dot Blot - Anti-GCN2 (phospho T899) antibody [EPR2320Y] (AB75836)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (101)

Product promise