Anti-GCN2 (phospho T899) antibody [EPR2320Y] is a rabbit recombinant monoclonal antibody designed to detect phosphorylated GCN2 at threonine 899. Validated for western blotting (WB) and dot blotting (Dot) applications. Suitable for human samples.
- Cited in over 75 publications
- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Trusted by researchers since 2009
Images
![Dot Blot - Anti-GCN2 (phospho T899) antibody [EPR2320Y] (AB75836), expandable thumbnail](https://content.abcam.com/products/images/gcn2-phospho-t899-antibody-epr2320y-ab75836--dot-blot-img15743.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-GCN2 (phospho T899) antibody [EPR2320Y] (AB75836)")
![Western blot - Anti-GCN2 (phospho T899) antibody [EPR2320Y] (AB75836), expandable thumbnail](https://content.abcam.com/products/images/gcn2-phospho-t899-antibody-epr2320y-ab75836--western-blot-img21538.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GCN2 (phospho T899) antibody [EPR2320Y] (AB75836)")
![Western blot - Anti-GCN2 (phospho T899) antibody [EPR2320Y] (AB75836), expandable thumbnail](https://content.abcam.com/products/images/gcn2-phospho-t899-antibody-epr2320y-ab75836--western-blot-img158937.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GCN2 (phospho T899) antibody [EPR2320Y] (AB75836)")
![Western blot - Anti-GCN2 (phospho T899) antibody [EPR2320Y] (AB75836), expandable thumbnail](https://content.abcam.com/products/images/gcn2-phospho-t899-antibody-epr2320y-ab75836--western-blot-img110840.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GCN2 (phospho T899) antibody [EPR2320Y] (AB75836)")
Publications
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- Nature communications 14:55352023Activation of the integrated stress response by inhibitors of its kinases.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaria Szaruga,Dino A Janssen,Claudia de Miguel,George Hodgson,Agnieszka Fatalska,Aleksandra P Pitera,Antonina Andreeva,Anne BertolottiPubMed 37684277
- Molecular carcinogenesis 62:1531-15452023Enzymatic depletion of l-Met using an engineered human enzyme as a novel therapeutic strategy for melanoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCarly S Wilder,Jennifer Chiou,Anna Battenhouse,Achinto Saha,Zhao Chen,Eunice Kim,Mohamed I Gadallah,Stefano Tiziani,George Georgiou,Everett Stone,John DiGiovanniPubMed 37378415
- Heliyon 9:e147992023Activating mutations in EGFR and PI3K promote ATF4 induction for NSCLC cell survival during amino acid deprivation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMizuki Takahashi,Yuka Okamoto,Yu Kato,Hitomi Shirahama,Satomi Tsukahara,Yoshikazu Sugimoto,Akihiro TomidaPubMed 37025861
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e228202023Cholic acid exposure during late pregnancy causes placental dysfunction and fetal growth restriction by reactive oxygen species-mediated activation of placental GCN2/eIF2α pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuai Lin,Meng-Ying Ye,Qian-Yun Fu,Chao-Lin Pan,Ya-Jie Liu,Li-Ming Zheng,Qiang Hong,Yuan-Hua ChenPubMed 36801982
- Translational oncology 27:1015922022The antitumor activity of a novel GCN2 inhibitor in head and neck squamous cell carcinoma cell lines.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJeongjae Lee,Bhumsuk Keam,Soyeon Kim,Jung-Nyoung Heo,Eunkyo Joung,Miso Kim,Tae Min Kim,Dong-Wan Kim,Dae Seog HeoPubMed 36436443
- Science advances 8:eabo13042022Ribosome changes reprogram translation for chemosurvival in G0 leukemic cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChandreyee Datta,Samuel S Truesdell,Keith Q Wu,Syed I A Bukhari,Harrison Ngue,Brienna Buchanan,Olivier Le Tonqueze,Sooncheol Lee,Swapna Kollu,Madeleine A Granovetter,Myriam Boukhali,Johannes Kreuzer,Maheen S Batool,Leonora Balaj,Wilhelm Haas,Shobha VasudevanPubMed 36306353
- The Journal of biological chemistry 298:1026292022A cell-based chemical-genetic screen for amino acid stress response inhibitors reveals torins reverse stress kinase GCN2 signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJohanna B Brüggenthies,Alessandra Fiore,Marion Russier,Christina Bitsina,Julian Brötzmann,Susanne Kordes,Sascha Menninger,Alexander Wolf,Elena Conti,Jan E Eickhoff,Peter J MurrayPubMed 36273589
- Cell death discovery 8:4062022Integrated stress response is involved in the 24(S)-hydroxycholesterol-induced unconventional cell death mechanism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYasuomi Urano,Shoya Osaki,Ren Chiba,Noriko NoguchiPubMed 36195595
- eLife 11:2022GCN2 eIF2 kinase promotes prostate cancer by maintaining amino acid homeostasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRicardo A Cordova,Jagannath Misra,Parth H Amin,Anglea J Klunk,Nur P Damayanti,Kenneth R Carlson,Andrew J Elmendorf,Hyeong-Geug Kim,Emily T Mirek,Bennet D Elzey,Marcus J Miller,X Charlie Dong,Liang Cheng,Tracy G Anthony,Roberto Pili,Ronald C Wek,Kirk A StaschkePubMed 36107759
- Cell metabolism 34:1151-1167.e72022GCN2 inhibition sensitizes arginine-deprived hepatocellular carcinoma cells to senolytic treatment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRindert Missiaen,Nicole M Anderson,Laura C Kim,Bailey Nance,Michelle Burrows,Nicolas Skuli,Madeleine Carens,Romain Riscal,An Steensels,Fuming Li,M Celeste SimonPubMed 35839757
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | Dot | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Expected | Tested | Not recommended | Not recommended |
| Synthetic peptide | Not recommended | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Synthetic peptide | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Synthetic peptide | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/2000 | Notes For unpurified use at 1/500. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Synthetic peptide | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Synthetic peptide | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) in response to low amino acid availability (PubMed:25329545, PubMed:32610081). Plays a role as an activator of the integrated stress response (ISR) required for adaptation to amino acid starvation (By similarity). EIF2S1/eIF-2-alpha phosphorylation in response to stress converts EIF2S1/eIF-2-alpha into a global protein synthesis inhibitor, leading to a global attenuation of cap-dependent translation, and thus to a reduced overall utilization of amino acids, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion (PubMed:32610081). Binds uncharged tRNAs (By similarity). Required for the translational induction of protein kinase PRKCH following amino acid starvation (By similarity). Involved in cell cycle arrest by promoting cyclin D1 mRNA translation repression after the unfolded protein response pathway (UPR) activation or cell cycle inhibitor CDKN1A/p21 mRNA translation activation in response to amino acid deprivation (PubMed:26102367). Plays a role in the consolidation of synaptic plasticity, learning as well as formation of long-term memory (By similarity). Plays a role in neurite outgrowth inhibition (By similarity). Plays a proapoptotic role in response to glucose deprivation (By similarity). Promotes global cellular protein synthesis repression in response to UV irradiation independently of the stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK) and p38 MAPK signaling pathways (By similarity). Plays a role in the antiviral response against alphavirus infection; impairs early viral mRNA translation of the incoming genomic virus RNA, thus preventing alphavirus replication (By similarity). (Microbial infection) Plays a role in modulating the adaptive immune response to yellow fever virus infection; promotes dendritic cells to initiate autophagy and antigene presentation to both CD4(+) and CD8(+) T-cells under amino acid starvation (PubMed:24310610).
Alternative names
GCN2, KIAA1338, EIF2AK4, eIF-2-alpha kinase GCN2, Eukaryotic translation initiation factor 2-alpha kinase 4, GCN2-like protein
Recommended products
Anti-GCN2 (phospho T899) antibody [EPR2320Y] is a rabbit recombinant monoclonal antibody designed to detect phosphorylated GCN2 at threonine 899. Validated for western blotting (WB) and dot blotting (Dot) applications. Suitable for human samples.
- Cited in over 75 publications
- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Trusted by researchers since 2009
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR2320Y
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-GCN2 (phospho T899) antibody [EPR2320Y] (ab75836) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Dot blot, WB in human samples.
Anti-GCN2 (phospho T899) antibody [EPR2320Y] (ab75836) specifically detects GCN2 Phospho-T899 (UniProt ID: Q9P2K8; Molecular weight: 187kDa) and is sold in 100 µL and 1 mL selling sizes.
Quality and Validation
Abcam's high quality manufacturing and validation processes ensure Anti-GCN2 (phospho T899) antibody [EPR2320Y] (ab75836) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-GCN2 (phospho T899) antibody [EPR2320Y] (ab75836) has been cited over 79 times in peer reviewed journals and is trusted by the scientific community.
Anti-GCN2 (phospho T899) antibody [EPR2320Y] (ab75836) has 12 independent reviews from customers.
Related Products
Conjugation-ready, carrier free format available for antibody clone EPR2320Y - Anti-beta III Tubulin antibody [EP1569Y] - BSA and Azide free ab221935.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Dot Blot - Anti-GCN2 (phospho T899) antibody [EPR2320Y] (ab75836), expandable thumbnail](https://content.abcam.com/products/images/gcn2-phospho-t899-antibody-epr2320y-ab75836--dot-blot-img15743.jpg "Dot Blot - Anti-GCN2 (phospho T899) antibody [EPR2320Y] (ab75836)")
Dot Blot - Anti-GCN2 (phospho T899) antibody [EPR2320Y] (ab75836)
Dot blot analysis of GCN2 (phospho T899) phospho peptide (Lane 1) and GCN2 non-phospho peptide (Lane 2) labelling GCN2 (phospho T899) phospho peptide with ab75836 at a dilution of 1:1000 dilution (0.277μg/ml). A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) was used as the secondary antibody at a dilution of 1:20,000 dilution. Blocking buffer: 5% NFDM/TBST. Dilution buffer: 5% NFDM /TBST .
![Western blot - Anti-GCN2 (phospho T899) antibody [EPR2320Y] (ab75836), expandable thumbnail](https://content.abcam.com/products/images/gcn2-phospho-t899-antibody-epr2320y-ab75836--western-blot-img21538.jpg "Western blot - Anti-GCN2 (phospho T899) antibody [EPR2320Y] (ab75836)")
Western blot - Anti-GCN2 (phospho T899) antibody [EPR2320Y] (ab75836)
Blocking and diluting buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-GCN2 (phospho T899) antibody [EPR2320Y] (ab75836) at 1/1000 dilution
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) grown in serum free media for 24hours, whole cell lysate at 15 µg
Lane 2: HeLa grown in serum free media for 24hours, then treated with 100nM Calyculin A for 30min, whole cell lysate at 15 µg
Lane 3: HeLa grown in serum free media for 24hours, then treated with 100nM Calyculin A for 30min, whole cell lysate. Then the membrane was incubated with alkaline phosphatase at 15 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 187 kDa
![Western blot - Anti-GCN2 (phospho T899) antibody [EPR2320Y] (ab75836), expandable thumbnail](https://content.abcam.com/products/images/gcn2-phospho-t899-antibody-epr2320y-ab75836--western-blot-img158937.jpg "Western blot - Anti-GCN2 (phospho T899) antibody [EPR2320Y] (ab75836)")
Western blot - Anti-GCN2 (phospho T899) antibody [EPR2320Y] (ab75836)
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
All lanes: Western blot - Anti-GCN2 (phospho T899) antibody [EPR2320Y] (ab75836) at 1/1000 dilution
Lane 1: HeLa cell lysate - untreated at 10 µg
Lane 2: HeLa cell lysate - treated with Calyculin at 10 µg
SecondaryAll lanes: Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 187 kDa
Observed band size: 220 kDa
![Western blot - Anti-GCN2 (phospho T899) antibody [EPR2320Y] (ab75836), expandable thumbnail](https://content.abcam.com/products/images/gcn2-phospho-t899-antibody-epr2320y-ab75836--western-blot-img110840.jpg "Western blot - Anti-GCN2 (phospho T899) antibody [EPR2320Y] (ab75836)")
Western blot - Anti-GCN2 (phospho T899) antibody [EPR2320Y] (ab75836)
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
All lanes: Western blot - Anti-GCN2 (phospho T899) antibody [EPR2320Y] (ab75836) at 1/500 dilution
Lane 1: HeLa cell lysate - untreated at 10 µg
Lane 2: HeLa cell lysate - treated with Calyculin A at 10 µg
SecondaryAll lanes: Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 187 kDa
Observed band size: 220 kDa
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