Anti-GCNA1 antibody [TRA98]

• IHC-Fr

AbReview53977****

Immunohistochemistry (Frozen sections) - Anti-GCNA1 antibody [TRA98] (AB82527)

ab82527 staining GCNA1 in adult mouse testis tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Triton and blocked with 3% BSA in PBS for 30 minutes at 20°C. Samples were incubated with primary antibody (1/1000) for 1 hour at 20°C. ab150165 was used as the secondary antibody, undiluted.

This image is courtesy of an Abreview by Bryan Niedenberger.

• WB

Unknown

Western blot - Anti-GCNA1 antibody [TRA98] (AB82527)

Western blot analysis of various mouse tissues, using ab82527.
In adult tissues, bands of 60-100 kDa proteins are detected only in the testis. The signals are observed in male and female embryos after embryonic day 12.5. The signal in male is detected during development of germ cells and also after birth, but the signal in female disappears by 5 days after birth. The antigen is localized only in the nuclear fraction of testicular germ cells.

All lanes:

Western blot - Anti-GCNA1 antibody [TRA98] (ab82527) at 0.1 µg/mL

Lane 1:

mouse brain lysate at 100 µg

Lane 2:

mouse heart lysate at 100 µg

Lane 3:

mouse intestine lysate at 100 µg

Lane 4:

mouse kidney lysate at 100 µg

Lane 5:

mouse liver lysate at 100 µg

Lane 6:

mouse lung lysate at 100 µg

Lane 7:

mouse spleen lysate at 100 µg

Lane 8:

mouse ovary lysate at 100 µg

Lane 9:

mouse testis lysate at 100 µg

Predicted band size: 146 kDa

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