Rat Monoclonal GCNA1 antibody. Suitable for IHC-Fr, WB and reacts with Mouse samples. Cited in 95 publications. Immunogen corresponding to Cell preparation containing GCNA1 protein.
Images
![Immunohistochemistry (Frozen sections) - Anti-GCNA1 antibody [TRA98] (AB82527), expandable thumbnail](https://content.abcam.com/products/images/gcna1-antibody-tra98-ab82527--immunohistochemistry-frozen-sections-img131858.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-GCNA1 antibody [TRA98] (AB82527)")
![Western blot - Anti-GCNA1 antibody [TRA98] (AB82527), expandable thumbnail](https://content.abcam.com/products/images/gcna1-antibody-tra98-ab82527--western-blot-img6711.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GCNA1 antibody [TRA98] (AB82527)")
Publications
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- Frontiers in cell and developmental biology 11:12841842023Core binding factor subunit β plays diverse and essential roles in the male germline.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMustika Rahmawati,Kassie M Stadler,Blanca Lopez-Biladeau,Tia M Hoisington,Nathan C LawPubMed 38020932
- Frontiers in cell and developmental biology 11:12704082023Transcription and chromatin regulation by TAF4b during cellular quiescence of developing prospermatogonia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMegan A Gura,Myles A Bartholomew,Kimberly M Abt,Soňa Relovská,Kimberly A Seymour,Richard N FreimanPubMed 37900284
- iScience 26:1079492023CD38 regulates ovarian function and fecundity via NAD metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRosalba Perrone,Prasanna Vadhana Ashok Kumaar,Lauren Haky,Cosmo Hahn,Rebeccah Riley,Julia Balough,Giuliana Zaza,Bikem Soygur,Kaitlyn Hung,Leandro Prado,Herbert G Kasler,Ritesh Tiwari,Hiroyuki Matsui,Genesis Vega Hormazabal,Indra Heckenbach,Morten Scheibye-Knudsen,Francesca E Duncan,Eric VerdinPubMed 37822499
- iScience 26:1076162023WNK1 is required during male pachynema to sustain fertility.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRu-Pin Alicia Chi,Xiaojiang Xu,Jian-Liang Li,Xin Xu,Guang Hu,Paula Brown,Cynthia Willson,Oleksandr Kirsanov,Christopher Geyer,Chou-Long Huang,Marcos Morgan,Francesco DeMayoPubMed 37694147
- Frontiers in cell and developmental biology 11:12372732023Spermatogonial fate in mice with increased activin A bioactivity and testicular somatic cell tumours.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPenny A F Whiley,Benedict Nathaniel,Peter G Stanton,Robin M Hobbs,Kate L LovelandPubMed 37564373
- Scientific reports 13:86712023Prenatal exposure to benzo[a]pyrene depletes ovarian reserve and masculinizes embryonic ovarian germ cell transcriptome transgenerationally.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinhwan Lim,Toshihiro Shioda,Kelli F Malott,Keiko Shioda,Junko Odajima,Kathleen N Leon Parada,Julie Nguyen,Samantha Getze,Melody Lee,Jonathon Nguyen,Samantha Reshel Blakeley,Vienna Trinh,Hong-An Truong,Ulrike LudererPubMed 37248279
- International journal of molecular sciences 24:2023Cocktails of NSAIDs and 17α Ethinylestradiol at Environmentally Relevant Doses in Drinking Water Alter Puberty Onset in Mice Intergenerationally.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPascal Philibert,Stéphanie Déjardin,Mélissa Girard,Quentin Durix,Anne-Alicia Gonzalez,Xavier Mialhe,Mathieu Tardat,Francis Poulat,Brigitte Boizet-BonhourePubMed 36982971
- Nucleic acids research 51:4791-48132023GCNA is a histone binding protein required for spermatogonial stem cell maintenance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJonathan Ribeiro,Gerry P CrossanPubMed 36919611
- PLoS genetics 19:e10106562023The RNA binding protein DND1 is elevated in a subpopulation of pro-spermatogonia and targets chromatin modifiers and translational machinery during late gestation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVictor A Ruthig,Talia Hatkevich,Josiah Hardy,Matthew B Friedersdorf,Chloé Mayère,Serge Nef,Jack D Keene,Blanche CapelPubMed 36857387
- Cell death & disease 14:1342023Single cell epigenomic and transcriptomic analysis uncovers potential transcription factors regulating mitotic/meiotic switch.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFa-Li Zhang,Yan-Qin Feng,Jing-Ya Wang,Ke-Xin Zhu,Lu Wang,Jia-Mao Yan,Xiu-Xiu Li,Jun-Jie Wang,Wei Ge,Massimo De Felici,Wei ShenPubMed 36797258
Key facts
- Isotype
- IgG2a
- Host species
- Rat
- Storage buffer
pH: 6 - 8.5
Constituents: 50% Glycerol (glycerin, glycerine), 50% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Cell preparation containing GCNA1 protein. The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-Fr | WB | |
|---|---|---|
| Mouse | Tested | Tested |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/5000 | Notes - |
Target data
Alternative names
GENA, Testicular germ cell-specific antigen
Recommended products
Rat Monoclonal GCNA1 antibody. Suitable for IHC-Fr, WB and reacts with Mouse samples. Cited in 95 publications. Immunogen corresponding to Cell preparation containing GCNA1 protein.
Key facts
- Isotype
- IgG2a
- Host species
- Rat
- Storage buffer
pH: 6 - 8.5
Constituents: 50% Glycerol (glycerin, glycerine), 50% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Cell preparation containing GCNA1 protein. The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- TRA98
- Specificity
In PubMed: 27718356 the epitope of ab82527 has been mapped to a murine-specific 8-amino-acid tandem GE(P/M/S)E(S/T)EAK repeat that occurs 25 times in mouse GCNA1 (aa 167- 383).
- Concentration
- Purification notes
This product was purified from serum-free culture medium of the hybridoma clone TRA98 by proprietary chromatography processes under mild conditions.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunohistochemistry (Frozen sections) - Anti-GCNA1 antibody [TRA98] (ab82527), expandable thumbnail](https://content.abcam.com/products/images/gcna1-antibody-tra98-ab82527--immunohistochemistry-frozen-sections-img131858.jpg "Immunohistochemistry (Frozen sections) - Anti-GCNA1 antibody [TRA98] (ab82527)")
This image is courtesy of a customer review by Bryan Niedenberger.Immunohistochemistry (Frozen sections) - Anti-GCNA1 antibody [TRA98] (ab82527)
ab82527 staining GCNA1 in adult mouse testis tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Triton and blocked with 3% BSA in PBS for 30 minutes at 20°C. Samples were incubated with primary antibody (1/1000) for 1 hour at 20°C. Goat Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed ab150165 was used as the secondary antibody, undiluted.
![Western blot - Anti-GCNA1 antibody [TRA98] (ab82527), expandable thumbnail](https://content.abcam.com/products/images/gcna1-antibody-tra98-ab82527--western-blot-img6711.jpg "Western blot - Anti-GCNA1 antibody [TRA98] (ab82527)")
Western blot - Anti-GCNA1 antibody [TRA98] (ab82527)
Western blot analysis of various mouse tissues, using ab82527.
In adult tissues, bands of 60-100 kDa proteins are detected only in the testis. The signals are observed in male and female embryos after embryonic day 12.5. The signal in male is detected during development of germ cells and also after birth, but the signal in female disappears by 5 days after birth. The antigen is localized only in the nuclear fraction of testicular germ cells.All lanes: Western blot - Anti-GCNA1 antibody [TRA98] (ab82527) at 0.1 µg/mL
Lane 1: mouse brain lysate at 100 µg
Lane 2: mouse heart lysate at 100 µg
Lane 3: mouse intestine lysate at 100 µg
Lane 4: mouse kidney lysate at 100 µg
Lane 5: mouse liver lysate at 100 µg
Lane 6: mouse lung lysate at 100 µg
Lane 7: mouse spleen lysate at 100 µg
Lane 8: mouse ovary lysate at 100 µg
Lane 9: mouse testis lysate at 100 µg
Predicted band size: 146 kDa
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