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AB153939

Anti-GCP4 antibody

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(1 Publication)

Rabbit Polyclonal GCP4 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human TUBGCP4 aa 50-350.

View Alternative Names

76P, GCP4, TUBGCP4, Gamma-tubulin complex component 4, GCP-4, hGCP4, Gamma-ring complex protein 76 kDa, h76p, hGrip76

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-GCP4 antibody (AB153939)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GCP4 antibody (AB153939)

Confocal Immunofluorescent analysis of paraformaldehyde-fixed 293T cells labeling GCP4 with ab153939 at 1/500 dilution (green). Alpha-tubulin filaments are labeled in red

Immunocytochemistry/ Immunofluorescence - Anti-GCP4 antibody (AB153939)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GCP4 antibody (AB153939)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling GCP4 protein at cytoplasm and centrosome using ab153939 at a 1/1000 dilution (Green).
alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody at a 1/1000 dilution (Red). Hoechst 33342 was used for nuclear staining (Blue).

Western blot - Anti-GCP4 antibody (AB153939)
  • WB

Unknown

Western blot - Anti-GCP4 antibody (AB153939)

10 % SDS-PAGE

All lanes:

Western blot - Anti-GCP4 antibody (ab153939) at 1/1000 dilution

All lanes:

NIH 3T3 whole cell lysate at 30 µg

Predicted band size: 76 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human TUBGCP4 aa 50-350. The exact immunogen used to generate this antibody is proprietary information.

Q9UGJ1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus tropicalis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the gamma-tubulin ring complex (gTuRC) which mediates microtubule nucleation (PubMed : 38305685, PubMed : 38609661, PubMed : 39321809). The gTuRC regulates the minus-end nucleation of alpha-beta tubulin heterodimers that grow into microtubule protafilaments, a critical step in centrosome duplication and spindle formation (PubMed : 38305685, PubMed : 38609661, PubMed : 39321809).
See full target information TUBGCP4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 133: PubMed37581935

2023

CFTR-rich ionocytes mediate chloride absorption across airway epithelia.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Lei,Soumba Traore,Guillermo S Romano Ibarra,Philip H Karp,Tayyab Rehman,David K Meyerholz,Joseph Zabner,David A Stoltz,Patrick L Sinn,Michael J Welsh,Paul B McCray,Ian M Thornell
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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