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AB95172

Anti-GCP6 antibody

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(7 Publications)

Rabbit Polyclonal GCP6 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human TUBGCP6 aa 1350-1400.

View Alternative Names

GCP6, KIAA1669, TUBGCP6, Gamma-tubulin complex component 6, GCP-6

2 Images
Western blot - Anti-GCP6 antibody (AB95172)
  • WB

Unknown

Western blot - Anti-GCP6 antibody (AB95172)

All lanes:

Western blot - Anti-GCP6 antibody (ab95172) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 200 kDa

false

Immunoprecipitation - Anti-GCP6 antibody (AB95172)
  • IP

Unknown

Immunoprecipitation - Anti-GCP6 antibody (AB95172)

Lane 1 : 3μg ab95172 were used to immunoprecipitate GCP3 from 1mg HeLa whole cell lysate. 20% IP was loaded per lane and probed with ab95170 at 1μg/ml.
Lane 2 : same, using a control IgG.
Detection : chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-GCP6 antibody (ab95172)

Predicted band size: 200 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human TUBGCP6 aa 1350-1400. The exact immunogen used to generate this antibody is proprietary information.

Q96RT7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome.
See full target information TUBGCP6
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

eLife 12: PubMed37737843

2023

CRB3 navigates Rab11 trafficking vesicles to promote γTuRC assembly during ciliogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Wang,Zheyong Liang,Tan Tan,Miao Zhang,Yina Jiang,Yangyang Shang,Xiaoqian Gao,Shaoran Song,Ruiqi Wang,He Chen,Jie Liu,Juan Li,Yu Ren,Peijun Liu

Cell reports 41:111642 PubMed36384111

2022

Α γ-tubulin complex-dependent pathway suppresses ciliogenesis by promoting cilia disassembly.

Applications

Unspecified application

Species

Unspecified reactive species

Sahana Shankar,Zi-Ting Hsu,Artur Ezquerra,Chien-Chien Li,Tzu-Lun Huang,Etienne Coyaud,Ricardo Viais,Cédric Grauffel,Brian Raught,Carmay Lim,Jens Lüders,Su-Yi Tsai,Kuo-Chiang Hsia

Biochimica et biophysica acta. Molecular cell research 1865:158-171 PubMed29050966

2017

Characterization of gamma-tubulin filaments in mammalian cells.

Applications

WB

Species

Unspecified reactive species

Lisa Lindström,Maria Alvarado-Kristensson

Journal of cell science 130:406-419 PubMed27852835

2016

MZT1 regulates microtubule nucleation by linking γTuRC assembly to adapter-mediated targeting and activation.

Applications

Unspecified application

Species

Unspecified reactive species

Rosa Ramírez Cota,Neus Teixidó-Travesa,Artur Ezquerra,Susana Eibes,Cristina Lacasa,Joan Roig,Jens Lüders

The Journal of biological chemistry 291:23112-23125 PubMed27660388

2016

Functional Analysis of γ-Tubulin Complex Proteins Indicates Specific Lateral Association via Their N-terminal Domains.

Applications

Unspecified application

Species

Unspecified reactive species

Dorian Farache,Alain Jauneau,Cécile Chemin,Marine Chartrain,Marie-Hélène Rémy,Andreas Merdes,Laurence Haren

The Journal of biological chemistry 289:31719-35 PubMed25246530

2014

TACC3 protein regulates microtubule nucleation by affecting γ-tubulin ring complexes.

Applications

Unspecified application

Species

Unspecified reactive species

Puja Singh,Geethu Emily Thomas,Koyikulangara K Gireesh,Tapas K Manna

Nature cell biology 16:770-8 PubMed24976384

2014

The dynamics of microtubule minus ends in the human mitotic spindle.

Applications

Unspecified application

Species

Unspecified reactive species

Nicolas Lecland,Jens Lüders
View all publications
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