AB233783

Anti-GCSF Receptor antibody [8F8B12]

Name: Anti-GCSF Receptor antibody [8F8B12] (ab233783) | Abcam
Brand: Abcam Limited
SKU: AB233783
Price: 495 USD
Availability: InStock

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(1 Publication)

Mouse Monoclonal GCSF Receptor antibody. Suitable for Flow Cyt, WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CSF3R aa 1-200.

View Alternative Names

CD114, GCSFR, CSF3R, Granulocyte colony-stimulating factor receptor, G-CSF receptor, G-CSF-R

5 Images

Flow Cytometry - Anti-GCSF Receptor antibody [8F8B12] (AB233783)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-GCSF Receptor antibody [8F8B12] (AB233783)

Flow cytometric analysis of Ramos (human Burkitt's lymphoma cell line) cell line labeling GCSF Receptor with ab233783 at 1/200 dilution (green) compared with a negative control (red).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCSF Receptor antibody [8F8B12] (AB233783)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCSF Receptor antibody [8F8B12] (AB233783)

Paraffin-embedded human bladder cancer tissue stained for GCSF Receptor using ab233783 at 1/200 dilution in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-GCSF Receptor antibody [8F8B12] (AB233783)

All lanes:

Western blot - Anti-GCSF Receptor antibody [8F8B12] (ab233783) at 1/500 dilution

Lane 1:

A431 (human epidermoid carcinoma cell line) whole cell lysate

Lane 2:

K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate

Lane 3:

THP-1 (human monocytic leukemia cell line) whole cell lysate

Lane 4:

MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate

Lane 5:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

Predicted band size: 92 kDa

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Supplier Data

Western blot - Anti-GCSF Receptor antibody [8F8B12] (AB233783)

Expected MW is 46.5 kDa.

Lane 1:

Western blot - Anti-GCSF Receptor antibody [8F8B12] (ab233783) at 1/500 dilution

Lane 1:

Western blot - Anti-BTN2A2/BTF2 antibody [6C7D2] (<a href='/en-us/products/primary-antibodies/btn2a2-btf2-antibody-6c7d2-ab233763'>ab233763</a>) at 1/500 dilution

All lanes:

Recombinant human GCSF receptor (AA: extra 25-187) protein

Predicted band size: 59 kDa,92 kDa

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Supplier Data

Western blot - Anti-GCSF Receptor antibody [8F8B12] (AB233783)

Lanes 1 - 2:

Western blot - Anti-BTN2A2/BTF2 antibody [6C7D2] (<a href='/en-us/products/primary-antibodies/btn2a2-btf2-antibody-6c7d2-ab233763'>ab233763</a>) at 1/500 dilution

Lanes 1 - 2:

Western blot - Anti-BTN2A2/BTF2 antibody [4B11C8] (<a href='/en-us/products/primary-antibodies/btn2a2-btf2-antibody-4b11c8-ab233752'>ab233752</a>) at 1/500 dilution

Lanes 1 - 2:

Western blot - Anti-GCSF Receptor antibody [8F8B12] (ab233783) at 1/500 dilution

Lane 1:

HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

Lane 2:

GCSF Receptor (AA: extra 25-187)-hIgGFc transfected HEK-293 cell lysate

Predicted band size: 59 kDa,92 kDa

true

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

8F8B12

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human CSF3R aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q99062

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from tissue culture supernatant.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for granulocyte colony-stimulating factor (CSF3), essential for granulocytic maturation. Plays a crucial role in the proliferation, differentiation and survival of cells along the neutrophilic lineage. In addition it may function in some adhesion or recognition events at the cell surface.

See full target information CSF3R

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biomolecules & biomedicine 24:848-856 PubMed38149831

2023

Hypomethylation of the promoter region contributes to the occurrence and development of ovarian endometriosis via the AKT/mTOR/4EBP1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yuzhen Qin,Yishuai Li,Yali Hao,Yan Li,Shan Kang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GCSF Receptor antibody [8F8B12]

Flow Cytometry - Anti-GCSF Receptor antibody [8F8B12] (AB233783)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCSF Receptor antibody [8F8B12] (AB233783)

Western blot - Anti-GCSF Receptor antibody [8F8B12] (AB233783)

Western blot - Anti-GCSF Receptor antibody [8F8B12] (AB233783)

Western blot - Anti-GCSF Receptor antibody [8F8B12] (AB233783)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

Product promise