Skip to main content

Mouse Monoclonal GCSF Receptor antibody. Suitable for Flow Cyt, WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CSF3R aa 1-200.

Be the first to review this product! Submit a review

Images

Flow Cytometry - Anti-GCSF Receptor antibody [8F8B12] (AB233783), expandable thumbnail
  • Western blot - Anti-GCSF Receptor antibody [8F8B12] (AB233783), expandable thumbnail
  • Western blot - Anti-GCSF Receptor antibody [8F8B12] (AB233783), expandable thumbnail
  • Western blot - Anti-GCSF Receptor antibody [8F8B12] (AB233783), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCSF Receptor antibody [8F8B12] (AB233783), expandable thumbnail

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

Preservative: 0.05% Sodium azide
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human CSF3R aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q99062

Abcam Recommends

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWBIHC-P
Human
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/200.00000 - 1/400.00000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500.00000 - 1/2000.00000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/200.00000 - 1/1000.00000
Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Receptor for granulocyte colony-stimulating factor (CSF3), essential for granulocytic maturation. Plays a crucial role in the proliferation, differentiation and survival of cells along the neutrophilic lineage. In addition it may function in some adhesion or recognition events at the cell surface.

Alternative names

CD114, GCSFR, CSF3R, Granulocyte colony-stimulating factor receptor, G-CSF receptor, G-CSF-R

Recommended products

Mouse Monoclonal GCSF Receptor antibody. Suitable for Flow Cyt, WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CSF3R aa 1-200.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Fragment Protein within Human CSF3R aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q99062
Clone number
8F8B12
Purification technique
Affinity purification Protein G
Concentration
Loading...
Purification notes

Purified from tissue culture supernatant.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Flow Cytometry - Anti-GCSF Receptor antibody [8F8B12] (ab233783), expandable thumbnail

    Flow Cytometry - Anti-GCSF Receptor antibody [8F8B12] (ab233783)

    Flow cytometric analysis of Ramos (human Burkitt's lymphoma cell line) cell line labeling GCSF Receptor with ab233783 at 1/200 dilution (green) compared with a negative control (red).

  • Western blot - Anti-GCSF Receptor antibody [8F8B12] (ab233783), expandable thumbnail

    Western blot - Anti-GCSF Receptor antibody [8F8B12] (ab233783)

    Lanes 1 - 2: Western blot - Anti-BTN2A2/BTF2 antibody [6C7D2] (Anti-BTN2A2/BTF2 antibody [6C7D2] ab233763) at 1/500 dilution

    Lanes 1 - 2: Western blot - Anti-BTN2A2/BTF2 antibody [4B11C8] (Anti-BTN2A2/BTF2 antibody [4B11C8] ab233752) at 1/500 dilution

    Lanes 1 - 2: Western blot - Anti-GCSF Receptor antibody [8F8B12] (ab233783) at 1/500 dilution

    Lane 1: HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

    Lane 2: GCSF Receptor (AA: extra 25-187)-hIgGFc transfected HEK-293 cell lysate

    Developed using the ECL technique.

    Predicted band size: 59 kDa, 92 kDa

  • Western blot - Anti-GCSF Receptor antibody [8F8B12] (ab233783), expandable thumbnail

    Western blot - Anti-GCSF Receptor antibody [8F8B12] (ab233783)

    All lanes: Western blot - Anti-GCSF Receptor antibody [8F8B12] (ab233783) at 1/500 dilution

    Lane 1: A431 (human epidermoid carcinoma cell line) whole cell lysate

    Lane 2: K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate

    Lane 3: THP-1 (human monocytic leukemia cell line) whole cell lysate

    Lane 4: MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate

    Lane 5: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

    Developed using the ECL technique.

    Predicted band size: 92 kDa

  • Western blot - Anti-GCSF Receptor antibody [8F8B12] (ab233783), expandable thumbnail

    Western blot - Anti-GCSF Receptor antibody [8F8B12] (ab233783)

    Expected MW is 46.5 kDa.

    Lane 1: Western blot - Anti-GCSF Receptor antibody [8F8B12] (ab233783) at 1/500 dilution

    Lane 1: Western blot - Anti-BTN2A2/BTF2 antibody [6C7D2] (Anti-BTN2A2/BTF2 antibody [6C7D2] ab233763) at 1/500 dilution

    All lanes: Recombinant human GCSF receptor (AA: extra 25-187) protein

    Developed using the ECL technique.

    Predicted band size: 59 kDa, 92 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCSF Receptor antibody [8F8B12] (ab233783), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCSF Receptor antibody [8F8B12] (ab233783)

    Paraffin-embedded human bladder cancer tissue stained for GCSF Receptor using ab233783 at 1/200 dilution in immunohistochemical analysis. DAB staining.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com