AB126167

Anti-GCSF Receptor antibody

Name: Anti-GCSF Receptor antibody (ab126167) | Abcam
Brand: Abcam Limited
SKU: AB126167

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(8 Publications)

Rabbit Polyclonal GCSF Receptor antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CSF3R aa 150-400.

View Alternative Names

CD114, GCSFR, CSF3R, Granulocyte colony-stimulating factor receptor, G-CSF receptor, G-CSF-R

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCSF Receptor antibody (AB126167)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCSF Receptor antibody (AB126167)

Formalin-fixed, paraffin embedded human breast carcinoma tissue stained fro GCSF receptor usinf a126167 at 1/250 dilution in immunohistochemical analysis,

Antigen Retrieval : EDTA based, pH 8.0 buffer, 15min

Immunocytochemistry/ Immunofluorescence - Anti-GCSF Receptor antibody (AB126167)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GCSF Receptor antibody (AB126167)

Immunofluorescence analysis of THP-1 (human monocytic leukemia cell line) cells labeling GCSF Receptor (green) using ab126167 at 1/500 dilution. Blue : Mounting medium with DAPI. Sample : THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.

Supplier Data

Western blot - Anti-GCSF Receptor antibody (AB126167)

7.5% SDS-PAGE

All lanes:

Western blot - Anti-GCSF Receptor antibody (ab126167) at 1/500 dilution

Lane 1:

Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

Lane 2:

Transfected HEK-293T whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 92 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human CSF3R aa 150-400. The exact immunogen used to generate this antibody is proprietary information.

Q99062

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for granulocyte colony-stimulating factor (CSF3), essential for granulocytic maturation. Plays a crucial role in the proliferation, differentiation and survival of cells along the neutrophilic lineage. In addition it may function in some adhesion or recognition events at the cell surface.

See full target information CSF3R

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

American journal of cancer research 14:3694-3710 PubMed39267669

2024

Paraneoplastic leukocytosis induces NETosis and thrombosis in bladder cancer PDX model.

Applications

Unspecified application

Species

Unspecified reactive species

Yung-Chia Kuo,Chen-Yang Huang,Cedric Chuan Young Ng,Chiao-Yun Lin,Wen-Kuan Huang,Li-Yu Lee,Hsien-Chi Fan,An-Chi Lin,Kai-Jie Yu,See-Tong Pang,Bin Tean Teh,Cheng-Lung Hsu

BMC oral health 23:422 PubMed37365568

2023

Effects of G-CSF on hPDLSC proliferation and osteogenic differentiation in the LPS-induced inflammatory microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Yu,Pengcheng Wang,Haibin Lu,Jiurong Guan,Fang Yao,Tianyi Zhang,Qiuxu Wang,Zuomin Wang

Frontiers in cell and developmental biology 8:601043 PubMed33415106

2020

Vitamin D Promotes Trophoblast Cell Induced Separation of Vascular Smooth Muscle Cells in Vascular Remodeling Induction of G-CSF.

Applications

Unspecified application

Species

Unspecified reactive species

Joy Yue Zhang,Peihuang Wu,Danyang Chen,Fen Ning,Qinsheng Lu,Xiu Qiu,Martin Hewison,Jennifer A Tamblyn,Mark D Kilby,Gendie E Lash

Scientific data 6:21 PubMed30967555

2019

Phospho serine and threonine analysis of normal and mutated granulocyte colony stimulating factor receptors.

Applications

Unspecified application

Species

Unspecified reactive species

Pankaj Dwivedi,David E Muench,Michael Wagner,Mohammad Azam,H Leighton Grimes,Kenneth D Greis

Haematologica 104:1789-1797 PubMed30819912

2019

mutations are recurrently acquired during chronic myeloid leukemia progression and interfere with myeloid differentiation pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Vera Magistroni,Mario Mauri,Deborah D'Aliberti,Caterina Mezzatesta,Ilaria Crespiatico,Miriam Nava,Diletta Fontana,Nitesh Sharma,Wendy Parker,Andreas Schreiber,David Yeung,Alessandra Pirola,Sara Readelli,Luca Massimino,Paul Wang,Praveen Khandelwal,Stefania Citterio,Michela Viltadi,Silvia Bombelli,Roberta Rigolio,Roberto Perego,Jacqueline Boultwood,Alessandro Morotti,Giuseppe Saglio,Dong-Wook Kim,Susan Branford,Carlo Gambacorti-Passerini,Rocco Piazza

Cancer research 77:4258-4267 PubMed28652245

2017

Unpaired Extracellular Cysteine Mutations of CSF3R Mediate Gain or Loss of Function.

Applications

Unspecified application

Species

Unspecified reactive species

Haijiao Zhang,Sophie Means,Anna Reister Schultz,Kevin Watanabe-Smith,Bruno C Medeiros,Daniel Bottomly,Beth Wilmot,Shannon K McWeeney,Tim Kükenshöner,Oliver Hantschel,Jeffrey W Tyner

Clinical cancer research : an official journal of the American Association for Cancer Research 22:757-64 PubMed26475333

2015

The Colony-Stimulating Factor 3 Receptor T640N Mutation Is Oncogenic, Sensitive to JAK Inhibition, and Mimics T618I.

Applications

Species

Human

Julia E Maxson,Samuel B Luty,Jason D MacManiman,Jason C Paik,Jason Gotlib,Peter Greenberg,Swaleh Bahamadi,Samantha L Savage,Melissa L Abel,Christopher A Eide,Marc M Loriaux,Emily A Stevens,Jeffrey W Tyner

The Journal of biological chemistry 289:5820-7 PubMed24403076

2014

Ligand independence of the T618I mutation in the colony-stimulating factor 3 receptor (CSF3R) protein results from loss of O-linked glycosylation and increased receptor dimerization.

Applications

Species

Human

Julia E Maxson,Samuel B Luty,Jason D MacManiman,Melissa L Abel,Brian J Druker,Jeffrey W Tyner

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GCSF Receptor antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCSF Receptor antibody (AB126167)

Immunocytochemistry/ Immunofluorescence - Anti-GCSF Receptor antibody (AB126167)

Western blot - Anti-GCSF Receptor antibody (AB126167)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (8)

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