Rabbit Polyclonal GCSF Receptor antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CSF3R aa 150-400.
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Publications
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- BMC oral health 23:4222023Effects of G-CSF on hPDLSC proliferation and osteogenic differentiation in the LPS-induced inflammatory microenvironment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHui Yu,Pengcheng Wang,Haibin Lu,Jiurong Guan,Fang Yao,Tianyi Zhang,Qiuxu Wang,Zuomin WangPubMed 37365568
- Frontiers in cell and developmental biology 8:6010432020Vitamin D Promotes Trophoblast Cell Induced Separation of Vascular Smooth Muscle Cells in Vascular Remodeling Induction of G-CSF.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJoy Yue Zhang et. al.PubMed 33415106
- Scientific data 6:212019Phospho serine and threonine analysis of normal and mutated granulocyte colony stimulating factor receptors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPankaj Dwivedi et. al.PubMed 30967555
- Haematologica 104:1789-17972019mutations are recurrently acquired during chronic myeloid leukemia progression and interfere with myeloid differentiation pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVera Magistroni et. al.PubMed 30819912
- Cancer research 77:4258-42672017Unpaired Extracellular Cysteine Mutations of CSF3R Mediate Gain or Loss of Function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaijiao Zhang et. al.PubMed 28652245
- Clinical cancer research : an official journal of the American Association for Cancer Research 22:757-642015The Colony-Stimulating Factor 3 Receptor T640N Mutation Is Oncogenic, Sensitive to JAK Inhibition, and Mimics T618I.Applications:WBReactive species:HumanJulia E Maxson,Samuel B Luty,Jason D MacManiman,Jason C Paik,Jason Gotlib,Peter Greenberg,Swaleh Bahamadi,Samantha L Savage,Melissa L Abel,Christopher A Eide,Marc M Loriaux,Emily A Stevens,Jeffrey W TynerPubMed 26475333
- The Journal of biological chemistry 289:5820-72014Ligand independence of the T618I mutation in the colony-stimulating factor 3 receptor (CSF3R) protein results from loss of O-linked glycosylation and increased receptor dimerization.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human CSF3R aa 150-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q99062
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Receptor for granulocyte colony-stimulating factor (CSF3), essential for granulocytic maturation. Plays a crucial role in the proliferation, differentiation and survival of cells along the neutrophilic lineage. In addition it may function in some adhesion or recognition events at the cell surface.
Alternative names
CD114, GCSFR, CSF3R, Granulocyte colony-stimulating factor receptor, G-CSF receptor, G-CSF-R
Recommended products
Rabbit Polyclonal GCSF Receptor antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CSF3R aa 150-400.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human CSF3R aa 150-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q99062
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCSF Receptor antibody (ab126167)
Formalin-fixed, paraffin embedded human breast carcinoma tissue stained fro GCSF receptor usinf a126167 at 1/250 dilution in immunohistochemical analysis,
Antigen Retrieval: EDTA based, pH 8.0 buffer, 15min
Immunocytochemistry/ Immunofluorescence - Anti-GCSF Receptor antibody (ab126167)
Immunofluorescence analysis of THP-1 (human monocytic leukemia cell line) cells labeling GCSF (green) using ab126167 at 1/500 dilution. Blue: Hoechst 33342 staining. Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Western blot - Anti-GCSF Receptor antibody (ab126167)
7.5% SDS-PAGE
All lanes: Western blot - Anti-GCSF Receptor antibody (ab126167) at 1/500 dilution
Lane 1: Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg
Lane 2: Transfected HEK-293T whole cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 92 kDa
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