Anti-GCSF Receptor antibody [EPR8957]

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-GCSF Receptor antibody [EPR8957] (AB156878)

Intracellular flow cytometric analysis of permeabilized NCCIT cells labeling GCSF Receptor using ab156878 antibody at a dilution of 1/500 (red) or a rabbit IgG (negative) (green).

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-GCSF Receptor antibody [EPR8957] (AB156878)

Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with ab156878 (right) or Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (left). Cells were fixed and permeabilised with BD Cytofix/Cytoperm™ for 20 min. PBMCs were incubated for 30 min at 4°C in 1x PBS containing 10 μg/ml human IgG and 10 % normal goat serum to block FC receptors and non-specific protein-protein interaction followed by ab156878 or Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (1x 106 in 100 μl at 0.2 μg/ml (1/8500)) for 30 min at 4°C . The cells were simultaneously stained with CD14.

The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/4000 for 30min at 4°C

Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on viable cells.

• WB

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Western blot - Anti-GCSF Receptor antibody [EPR8957] (AB156878)

All lanes:

Western blot - Anti-GCSF Receptor antibody [EPR8957] (ab156878) at 1/1000 dilution

Lane 1:

Human placenta extract at 10 µg

Lane 2:

NCCIT cell lysate at 10 µg

Lane 3:

Caco-2 cell lysate at 10 µg

Predicted band size: 92 kDa

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• I-ELISA

Lab

Indirect ELISA - Anti-GCSF Receptor antibody [EPR8957] (AB156878)

ELISA analysis of GCSF Receptor recombinant protein at 1000 ng/ml with ab156878. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 was used as the secondary antibody

• OI-RD Scanning

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OI-RD Scanning - Anti-GCSF Receptor antibody [EPR8957] (AB156878)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.