Anti-GDA antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDA antibody (AB233221)

Formalin-fixed, paraffin-embedded human stomach tissue stained for GDA using ab233221 at 30 μg/ml in immunohistochemical analysis.

Secondary used : HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 μg/ml. DAB staining.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDA antibody (AB233221)

Formalin-fixed, paraffin-embedded human liver tissue stained for GDA using ab233221 at 30 μg/ml in immunohistochemical analysis.

Secondary used : HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 μg/ml. DAB staining.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDA antibody (AB233221)

Formalin-fixed, paraffin-embedded human brain tissue stained for GDA using ab233221 at 30 μg/ml in immunohistochemical analysis.

Secondary used : HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 μg/ml. DAB staining.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDA antibody (AB233221)

Formalin-fixed, paraffin-embedded human liver cancer tissue stained for GDA using ab233221 at 30 μg/ml in immunohistochemical analysis.

Secondary used : HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 μg/ml. DAB staining.

• WB

Unknown

Western blot - Anti-GDA antibody (AB233221)

All lanes:

Western blot - Anti-GDA antibody (ab233221) at 1 µg/mL

Lane 1:

Human serum

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

Lane 3:

Mouse brain lysate

Lane 4:

Mouse kidney lysate

Lane 5:

Cow brain lysate

Secondary

All lanes:

HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution

Predicted band size: 51 kDa

false

• WB

Lab

Western blot - Anti-GDA antibody (AB233221)

False colour image of Western blot : Anti-GDA antibody staining at 0.5 μg/ml, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab233221 was shown to bind specifically to GDA. A band was observed at 51 kDa in wild-type HeLa cell lysates with no signal observed at this size in GDA knockout cell line ab264695 (knockout cell lysate ab258431). To generate this image, wild-type and GDA knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-GDA antibody (ab233221) at 0.5 µg/mL

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human GDA knockout HeLa cell lysate (<a href='/en-us/products/cell-lysates/human-gda-knockout-hela-cell-lysate-ab258431'>ab258431</a>)

Lane 2:

GDA knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human GDA knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-gda-knockout-hela-cell-line-ab264695'>ab264695</a>)

Lane 3:

Mouse kidney cell lysate at 20 µg

Lane 4:

Daudi cell lysate at 20 µg

Predicted band size: 51 kDa

Observed band size: 51 kDa

false

• WB

Unknown

Western blot - Anti-GDA antibody (AB233221)

All lanes:

Western blot - Anti-GDA antibody (ab233221) at 1 µg/mL

All lanes:

Recombinant human GDA protein

Secondary

All lanes:

HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution

Predicted band size: 51 kDa

false