Anti-GDAP1 antibody [EPR29581-48]

22 product images

• 

  Western blot - Anti-GDAP1 antibody [EPR29581-48] (ab323844)

  GDAP1 Western blot staining using rabbit Anti-GDAP1 antibody

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  This antibody does not cross-react with human GDAP1L1.

  In Western blot, Anti-His tag antibody - (Anti-6X His tag® antibody [AD1.1.10] ab15149) staining at 1/1000 dilution.

  All lanes: Western blot - Anti-GDAP1 antibody [EPR29581-48] (ab323844) at 1/1000 dilution

  Lane 1: Purified 293T cells transfected with a human GDAP1 expression vector containing a His-tag at 20 µg

  Lane 2: Purified 293T cells transfected with a human GDAP1L1 expression vector containing a His-tag at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Observed band size: 37 kDa

  Exposure time: 180s

• 

  Western blot - Anti-GDAP1 antibody [EPR29581-48] (ab323844)

  GDAP1 Western blot staining using rabbit Anti-GDAP1 antibody

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  Low expression: HT-29, 786-O, F9.

  The identity of the higher MW band at approximately 70 kDa is unknown.

  In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

  All lanes: Western blot - Anti-GDAP1 antibody [EPR29581-48] (ab323844) at 1/1000 dilution

  Lane 1: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg

  Lane 2: A375 (human malignant melanoma epithelial cell) whole cell lysate at 20 µg

  Lane 3: HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

  Lane 4: 786-O (human kidney epithelial cell) whole cell lysate at 20 µg

  Lane 5: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

  Lane 6: F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  Observed band size: 37 kDa, 36 kDa

  Exposure time: 180s

• 

  Western blot - Anti-GDAP1 antibody [EPR29581-48] (ab323844)

  GDAP1 Western blot staining using rabbit Anti-GDAP1 antibody

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  Low expression: liver, lung.

  The identity of the higher MW band at approximately 70 kDa is unknown.

  In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

  All lanes: Western blot - Anti-GDAP1 antibody [EPR29581-48] (ab323844) at 1/1000 dilution

  Lane 1: Mouse brain tissue lysate at 20 µg

  Lane 2: Mouse liver tissue lysate at 20 µg

  Lane 3: Mouse lung tissue lysate at 20 µg

  Lane 4: Rat brain tissue lysate at 20 µg

  Lane 5: Rat liver tissue lysate at 20 µg

  Lane 6: Rat lung tissue lysate at 20 µg

  Lane 7: Human hippocampus tissue lysate at 20 µg

  Lane 8: Human lung tissue lysate at 20 µg

  Lane 9: Human liver tissue lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  Observed band size: 37 kDa, 36 kDa

  Exposure time: 15s

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDAP1 antibody [EPR29581-48] (ab323844)

  GDAP1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human cerebrum using rabbit Anti-GDAP1 antibody

  Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling GDAP1 with ab323844 at 1/5000 (0.101 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining in human cerebrum.
  
  The primary antibody was incubated for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
  
  Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDAP1 antibody [EPR29581-48] (ab323844)

  GDAP1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human astrocytoma using rabbit Anti-GDAP1 antibody

  Immunohistochemical analysis of paraffin-embedded Human astrocytoma tissue labeling GDAP1 with ab323844 at 1/5000 (0.101 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining in human astrocytoma.
  
  The primary antibody was incubated for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
  
  Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDAP1 antibody [EPR29581-48] (ab323844)

  GDAP1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human breast cancer using rabbit Anti-GDAP1 antibody

  Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling GDAP1 with ab323844 at 1/5000 (0.101 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining in human breast cancer.
  
  The primary antibody was incubated for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
  
  Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDAP1 antibody [EPR29581-48] (ab323844)

  GDAP1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human pancreas using rabbit Anti-GDAP1 antibody

  Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling GDAP1 with ab323844 at 1/5000 (0.101 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining in human pancreas islet.
  
  The primary antibody was incubated for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
  
  Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDAP1 antibody [EPR29581-48] (ab323844)

  GDAP1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse cerebrum using rabbit Anti-GDAP1 antibody

  Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling GDAP1 with ab323844 at 1/1000 (0.504 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining in mouse cerebrum.
  
  The primary antibody was incubated for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
  
  Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDAP1 antibody [EPR29581-48] (ab323844)

  GDAP1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Rat cerebrum using rabbit Anti-GDAP1 antibody

  Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling GDAP1 with ab323844 at 1/1000 (0.504 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining in rat cerebrum.
  
  The primary antibody was incubated for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
  
  Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDAP1 antibody [EPR29581-48] (ab323844)

  GDAP1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human liver using rabbit Anti-GDAP1 antibody

  Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling GDAP1 with ab323844 at 1/5000 (0.101 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Low expression tissue: No staining in human liver.
  
  The primary antibody was incubated for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
  
  Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDAP1 antibody [EPR29581-48] (ab323844)

  GDAP1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse liver using rabbit Anti-GDAP1 antibody

  Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling GDAP1 with ab323844 at 1/1000 (0.504 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Low expression tissue: No staining in mouse liver.
  
  The primary antibody was incubated for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
  
  Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDAP1 antibody [EPR29581-48] (ab323844)

  GDAP1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Rat liver using rabbit Anti-GDAP1 antibody

  Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling GDAP1 with ab323844 at 1/1000 (0.504 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Low expression tissue: No staining in rat liver.
  
  The primary antibody was incubated for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
  
  Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunocytochemistry/ Immunofluorescence - Anti-GDAP1 antibody [EPR29581-48] (ab323844)

  GDAP1 Immunocytochemistry/ Immunofluorescence staining of mouse primary neural/glia cells using rabbit Anti-GDAP1 antibody

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cells labelling GDAP1 with ab323844 at 1/100 (5.04 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2ug/ml) dilution (Green).

  Confocal image showing cytoplasmic staining in mouse primary neuron (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  

  Anti-MAP2 antibody [HM-2] - Neuronal Marker ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 (4ug/ml) dilution (Magenta).

  -ve control 1: ab323844 at 1/100 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution.

  -ve control 2: Anti-MAP2 antibody [HM-2] - Neuronal Marker ab11267 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-GDAP1 antibody [EPR29581-48] (ab323844)

  GDAP1 Immunocytochemistry/ Immunofluorescence staining of mouse splenocytes using rabbit Anti-GDAP1 antibody

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse splenocytes labelling GDAP1 with ab323844 at 1/100 (5.04 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2ug/ml) dilution (Green).

  Low expression: Confocal image showing no staining in mouse splenocytes (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  

  Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 200 2.5ug/ml dilution (Magenta).

  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2ug/ml) dilution.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-GDAP1 antibody [EPR29581-48] (ab323844)

  GDAP1 Immunocytochemistry/ Immunofluorescence staining of rat primary neural/glia cells using rabbit Anti-GDAP1 antibody

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural/glia cells labelling GDAP1 with ab323844 at 1/100 (5.04 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2ug/ml) dilution (Green).

  Confocal image showing cytoplasmic staining in rat primary neuron (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  

  Anti-MAP2 antibody [HM-2] - Neuronal Marker ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 (4ug/ml) dilution (Magenta).

  -ve control 1: ab323844 at 1/100 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution.

  -ve control 2: Anti-MAP2 antibody [HM-2] - Neuronal Marker ab11267 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-GDAP1 antibody [EPR29581-48] (ab323844)

  GDAP1 Immunocytochemistry/ Immunofluorescence staining of rat splenocytes using rabbit Anti-GDAP1 antibody

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat splenocytes labelling GDAP1 with ab323844 at 1/100 (5.04 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2ug/ml) dilution (Green).

  Low expression: Confocal image showing no staining in rat splenocytes (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  

  Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Magenta).

  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2ug/ml) dilution.

• 

  Immunohistochemistry (Frozen sections) - Anti-GDAP1 antibody [EPR29581-48] (ab323844)

  GDAP1 Immunohistochemistry (Frozen sections) staining of Mouse cerebrum (perfused fixed) using rabbit Anti-GDAP1 antibody

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum (perfused fixed) tissue labeling GDAP1 with ab323844 at 1/50 (10.08 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).

  Panel A: merged staining of anti-GDAP1 (ab323844, green), anti-NeuN (Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, white) and anti-GFAP (Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker ab201732, magenta) on mouse cerebrum.
  
  Panel B: anti-GDAP1 stained on mouse cerebrum.
  
  Panel C: anti-NeuN stained in neurons of mouse cerebrum.
  
  Panel D: anti-GFAP stained in astrocytes of mouse cerebrum.
  
  The section was incubated in two rounds of staining: in the order of ab323844 and Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.

  Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

• 

  Immunohistochemistry (Frozen sections) - Anti-GDAP1 antibody [EPR29581-48] (ab323844)

  GDAP1 Immunohistochemistry (Frozen sections) staining of Mouse lung (perfused fixed) using rabbit Anti-GDAP1 antibody

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse lung (perfused fixed) tissue labeling GDAP1 with ab323844 at 1/50 (10.08 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).

  Low expression: confocal image showing no staining on mouse lung. The nuclear counterstain was DAPI (Blue). The section was incubated with ab323844 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.

  Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

• 

  Immunohistochemistry (Frozen sections) - Anti-GDAP1 antibody [EPR29581-48] (ab323844)

  GDAP1 Immunohistochemistry (Frozen sections) staining of Mouse liver (perfused fixed) using rabbit Anti-GDAP1 antibody

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver (perfused fixed) tissue labeling GDAP1 with ab323844 at 1/50 (10.08 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).

  Low expression: confocal image showing no staining on mouse liver. The nuclear counterstain was DAPI (Blue). The section was incubated with ab323844 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.

  Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

• 

  Immunohistochemistry (Frozen sections) - Anti-GDAP1 antibody [EPR29581-48] (ab323844)

  GDAP1 Immunohistochemistry (Frozen sections) staining of Rat cerebrum (perfused fixed) using rabbit Anti-GDAP1 antibody

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebrum (perfused fixed) tissue labeling GDAP1 with ab323844 at 1/50 (10.08 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).

  Panel A: merged staining of anti-GDAP1 (ab323844, green), anti-NeuN (Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, white) and anti-GFAP (Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker ab201732, magenta) on rat cerebrum.
  
  Panel B: anti-GDAP1 stained on rat cerebrum.
  
  Panel C: anti-NeuN stained in neurons of rat cerebrum.
  
  Panel D: anti-GFAP stained in astrocytes of rat cerebrum.
  
  The section was incubated in two rounds of staining: in the order of ab323844 and Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.

  Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

• 

  Immunohistochemistry (Frozen sections) - Anti-GDAP1 antibody [EPR29581-48] (ab323844)

  GDAP1 Immunohistochemistry (Frozen sections) staining of Rat lung (perfused fixed) using rabbit Anti-GDAP1 antibody

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat lung (perfused fixed) tissue labeling GDAP1 with ab323844 at 1/50 (10.08 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).

  Low expression: confocal image showing no staining on rat lung. The nuclear counterstain was DAPI (Blue). The section was incubated with ab323844 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.

  Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

• 

  Immunohistochemistry (Frozen sections) - Anti-GDAP1 antibody [EPR29581-48] (ab323844)

  GDAP1 Immunohistochemistry (Frozen sections) staining of Rat liver (perfused fixed) using rabbit Anti-GDAP1 antibody

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat liver (perfused fixed) tissue labeling GDAP1 with ab323844 at 1/50 (10.08 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).

  Low expression: confocal image showing no staining on rat liver. The nuclear counterstain was DAPI (Blue). The section was incubated with ab323844 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.

  Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).