AB93892

Anti-GDF 9 antibody

Name: Anti-GDF 9 antibody (ab93892) | Abcam
Brand: Abcam Limited
SKU: AB93892
Rating: 4 (8 reviews)

BOND RX™ Validated
What is this?

(8 Reviews)

(31 Publications)

Rabbit Polyclonal GDF 9 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 31 publications.

View Alternative Names

Growth/differentiation factor 9, GDF-9, GDF9

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF 9 antibody (AB93892)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF 9 antibody (AB93892)

IHC image of GDF 9 staining in human normal testis FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab93892, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX

Immunocytochemistry/ Immunofluorescence - Anti-GDF 9 antibody (AB93892)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-GDF 9 antibody (AB93892)

ab93892 staining GDF 9 in Jurkat cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab93892 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor^® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor^® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

IHC-P

AbReview37193****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF 9 antibody (AB93892)

ab93892 staining GDF 9 in Rat ovary tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 1.5% serum for 1 hour at 24°C; antigen retrieval was enzymatic. Samples were incubated with primary antibody (1μg/ml) for 12 hours at 4°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

This image is courtesy of an anonymous Abreview

Unknown

Western blot - Anti-GDF 9 antibody (AB93892)

All lanes:

Western blot - Anti-GDF 9 antibody (ab93892) at 1 µg/mL

All lanes:

Human ovary tissue lysate - total protein (<a href='/en-us/products/unavailable/human-ovary-tissue-lysate-total-protein-ab30222'>ab30222</a>) at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 51 kDa

Observed band size: 51 kDa

true

Exposure time: 2min

IHC-P

AbReview32337****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF 9 antibody (AB93892)

Immunohistochemical analysis of oocytes of primordial (A), and early antral follicles (B) of sheep ovary tissue, staining GDF 9 with ab93892.

Tissue was fixed with paraformaldehyde and blocked with 0.4% blocking agent for 5 minutes at 20°C; antigen retrieval was by heat mediation in citrate buffer (pH 6). Samples were incubated with primary antibody (1/1500 in diluent) for 24 hours at 4°C. An undiluted HRP-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody.

This image is courtesy of an Abreview submitted by Dr Rosa Ana Picazo

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Baboon": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Common marmoset": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Goat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Sheep": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required for ovarian folliculogenesis. Promotes primordial follicle development. Stimulates granulosa cell proliferation. Promotes cell transition from G0/G1 to S and G2/M phases, through an increase of CCND1 and CCNE1 expression, and RB1 phosphorylation. It regulates STAR expression and cAMP-dependent progesterone release in granulosa and thecal cells. Attenuates the suppressive effects of activin A on STAR expression and progesterone production by increasing the expression of inhibin B. It suppresses FST and FSTL3 production in granulosa-lutein cells.

See full target information GDF9

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Publications (31)

Recent publications for all applications. Explore the full list and refine your search

Reproductive sciences (Thousand Oaks, Calif.) 32:2970-2984 PubMed40789984

2025

Kuntai Capsules Improve Premature Ovarian Failure by Regulating AMPK-Mediated Autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaomin Ye,Miao Chen,Jiajing Zhong,Haofan Chen,Xinmiao Lin

International journal of reproductive biomedicine 23:33-44 PubMed40190455

2025

Cumulus cells conditioned medium facilitates germ cell differentiation from human embryonic stem cells: An experimental study.

Applications

Unspecified application

Species

Unspecified reactive species

Somayyeh Sadat Tahajjodi,Ehsan Farashahi Yazd,Azam Agharahimi,Fatemeh Akyash,Fatemeh Hajizadeh-Tafti,Jalal Golzadeh,Reza Aflatoonian,Behrouz Aflatoonian

Cell communication and signaling : CCS 22:235 PubMed38643161

2024

GDF9 and GDF9 bi-allelic variants caused female subfertility with defective follicle enlargement.

Applications

Unspecified application

Species

Unspecified reactive species

Yuwei Duan,Bing Cai,Jing Guo,Chen Wang,Qingyun Mai,Yan Xu,Yang Zeng,Yue Shi,Boyan Wang,Chenhui Ding,Minghui Chen,Canquan Zhou,Yanwen Xu

BioImpacts : BI 13:229-240 PubMed37431484

2023

Differentiation of human endometrial stem cells encapsulated in alginate hydrogel into oocyte-like cells.

Applications

Unspecified application

Species

Unspecified reactive species

Diba Ghasemi,Somayeh Ebrahimi-Barough,Mohammad Hossein Nekoofar,Abdolreza Mohamadnia,Nasrin Lotfibakhshaiesh,Naghmeh Bahrami,Roya Karimi,Vajihe Taghdiri Nooshabadi,Mahmoud Azami,Elham Hasanzadeh,Jafar Ai

Antioxidants (Basel, Switzerland) 11: PubMed36290750

2022

Tannin Reduces the Incidence of Polyspermic Penetration in Porcine Oocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Jing-Tao Sun,Jia-Hui Liu,Xi-Qing Jiang,Xin Luo,Jin-Dong Yuan,Qi Zhang,Xin-Yue Qi,Sanghoon Lee,Zhong-Hua Liu,Jun-Xue Jin

Journal of ovarian research 15:112 PubMed36242049

2022

Proteomic analysis implicates that postovulatory aging leads to aberrant gene expression, biosynthesis, RNA metabolism and cell cycle in mouse oocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Chuanxin Zhang,Xueqi Dong,Xinyi Yuan,Jinzhu Song,Jiawei Wang,Boyang Liu,Keliang Wu

Antioxidants (Basel, Switzerland) 11: PubMed36139716

2022

Ramelteon Reduces Oxidative Stress by Maintenance of Lipid Homeostasis in Porcine Oocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Jing-Tao Sun,Jin-Dong Yuan,Qi Zhang,Xin Luo,Xin-Yue Qi,Jia-Hui Liu,Xi-Qing Jiang,Sanghoon Lee,Anukul Taweechaipaisankul,Zhong-Hua Liu,Jun-Xue Jin

International journal of molecular sciences 23: PubMed35269923

2022

Long-Term Changes in Ovarian Follicles of Gilts Exposed Neonatally to Methoxychlor: Effects on Oocyte-Derived Factors, Anti-Müllerian Hormone, Follicle-Stimulating Hormone, and Cognate Receptors.

Applications

Unspecified application

Species

Unspecified reactive species

Patrycja Witek,Małgorzata Grzesiak,Marek Koziorowski,Maria Slomczynska,Katarzyna Knapczyk-Stwora

Cell death & disease 13:21 PubMed34930897

2021

CPEB3 deficiency in mice affect ovarian follicle development and causes premature ovarian insufficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Fang E,He Zhang,Wanli Yin,Chongyang Wang,Yuanli Liu,Yanze Li,Linlin Wang,Yue Wu,Runze Zhang,Chendan Zou,Tianjun Song,Cedric Matunda,Chaoxia Zou,Xu Gao

Antioxidants (Basel, Switzerland) 10: PubMed34679729

2021

Tannin Supplementation Improves Oocyte Cytoplasmic Maturation and Subsequent Embryo Development in Pigs.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi Yin,Jing-Tao Sun,Hong-Di Cui,Chao-Qian Jiang,Yu-Ting Zhang,Sanghoon Lee,Zhong-Hua Liu,Jun-Xue Jin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GDF 9 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF 9 antibody (AB93892)

Immunocytochemistry/ Immunofluorescence - Anti-GDF 9 antibody (AB93892)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF 9 antibody (AB93892)

Western blot - Anti-GDF 9 antibody (AB93892)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF 9 antibody (AB93892)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (31)

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