Rabbit Polyclonal GDF10 antibody. Suitable for WB, IHC-P and reacts with Rat, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GDF10 aa 350 to C-terminus.
Images
Publications
Filter by
- Cell death & disease 12:1562021Role of microRNA-335 carried by bone marrow mesenchymal stem cells-derived extracellular vesicles in bone fracture recovery.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaifeng Hu,Dong Wang,Lihong Li,Haiyang Yin,Guoyu He,Yonghong ZhangPubMed 33542183
- Journal of translational medicine 19:122021PGM5-AS1 impairs miR-587-mediated GDF10 inhibition and abrogates progression of prostate cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLei Du,Yongli GaoPubMed 33407592
- Aging 11:3298-33142019GDF10 inhibits proliferation and epithelial-mesenchymal transition in triple-negative breast cancer via upregulation of Smad7.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTian Zhou,Lei Yu,Jianjun Huang,Xueke Zhao,Yanwen Li,Yaxin Hu,Yu LeiPubMed 31147529
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human GDF10 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P55107
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Expected | Tested |
| Mouse | Predicted | Predicted |
| Rat | Tested | Expected |
| Cow | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/500.00000 - 1/5000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/200.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow | Dilution info - | Notes - |
Target data
Function
Growth factor involved in osteogenesis and adipogenesis. Plays an inhibitory role in the process of osteoblast differentiation via SMAD2/3 pathway. Plays an inhibitory role in the process of adipogenesis.
Alternative names
BMP3B, GDF10, Growth/differentiation factor 10, GDF-10, Bone morphogenetic protein 3B, Bone-inducing protein, BMP-3B, BIP
Recommended products
Rabbit Polyclonal GDF10 antibody. Suitable for WB, IHC-P and reacts with Rat, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GDF10 aa 350 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human GDF10 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P55107
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
Purity >95%
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF10 antibody (ab235005)
Paraffin-embedded human testis tissue stained for GDF10 using ab235005 at 1/200 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
Western blot - Anti-GDF10 antibody (ab235005)
All lanes: Western blot - Anti-GDF10 antibody (ab235005) at 1/500 dilution
All lanes: Rat brain tissue lysate.
SecondaryAll lanes: Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 53 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF10 antibody (ab235005)
Paraffin-embedded human prostate tissue stained for GDF10 using ab235005 at 1/200 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com