Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)]

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (AB124721)

Immunocytochemistry/Immunofluorescence analysis of A549 cells labelling BMP11 + GDF8/Myostatin with purified ab124721 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% triton X-100. ab150078, an Alexa Fluor^® 555-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

Control : primary antibody (1/250) and secondary antibody, ab150113, an Alexa Fluor^® 488-conjugated goat anti-mouse IgG (1/500).

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (AB124721)

ab124721 staining GDF11 + GDF8/Myostatin in the human cell line HeLa (human cervix adenocarcinoma) by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde, permabilised with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/250. A goat anti rabbit IgG (Alexa Fluor^® 488) at a dilution of 1/2000 was used as the secondary antibody.

Isoytype control : Rabbit monoclonal IgG (Black)

Unlabelled control : Cell without incubation with primary antibody and secondary antibody (Blue)

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (AB124721)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon adenocarcinoma tissue labelling BMP11 + GDF8/Myostatin with unpurified ab124721 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (AB124721)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebral cortex tissue labelling BMP11 + GDF8/Myostatin with purified ab124721 at 1/2500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

• WB

Supplier Data

Western blot - Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (AB124721)

Blocking and diluting buffer : 5% NFDM/TBST This antibody is unsuitable to detect tissue samples.

All lanes:

Western blot - Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (ab124721) at 1/1000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 3:

C2C12 (Mouse myoblasts myoblast) whole cell lysate at 20 µg

Lane 4:

C6 (Rat glial tumor glial cell) whole cell lysate at 20 µg

Lane 5:

Rat liver lysate at 20 µg

Lane 6:

Rat heart lysate at 20 µg

Lane 7:

Rat kidney lysate at 20 µg

Lane 8:

Mouse brain lysate at 20 µg

Lane 9:

Mouse liver lysate at 20 µg

Lane 10:

Human liver lysate at 20 µg

Lane 11:

Human skeletal muscle lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 45 kDa

false

Exposure time: 5s

• OI-RD Scanning

Unknown

OI-RD Scanning - Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (AB124721)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.