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Rabbit Recombinant Monoclonal GDF3 antibody. Carrier free. Suitable for WB and reacts with Human samples.

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Images

Western blot - Anti-GDF3 antibody [EPR4828] - BSA and Azide free (AB247699), expandable thumbnail
  • OI-RD Scanning - Anti-GDF3 antibody [EPR4828] - BSA and Azide free (AB247699), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Predicted
Rat
Predicted

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Growth factor involved in early embryonic development and adipose-tissue homeostasis. During embryogenesis controls formation of anterior visceral endoderm and mesoderm and the establishment of anterior-posterior identity through a receptor complex comprising the receptor ACVR1B and the coreceptor CRIPTO (By similarity). Regulates adipose-tissue homeostasis and energy balance under nutrient overload in part by signaling through the receptor complex based on ACVR1C and CRIPTO/Cripto (PubMed:21805089).

Alternative names

UNQ222/PRO248, GDF3, Growth/differentiation factor 3, GDF-3

Recommended products

Rabbit Recombinant Monoclonal GDF3 antibody. Carrier free. Suitable for WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR4828
Purification technique
Affinity purification
Dissociation constant
5.5 x 10-11 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab247699 is the carrier-free version of Anti-GDF3 antibody [EPR4828] ab108617.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-GDF3 antibody [EPR4828] - BSA and Azide free (ab247699), expandable thumbnail

    Western blot - Anti-GDF3 antibody [EPR4828] - BSA and Azide free (ab247699)

    This data was developed using Anti-GDF3 antibody [EPR4828] ab108617, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-GDF3 antibody [EPR4828] (Anti-GDF3 antibody [EPR4828] ab108617) at 1/1000 dilution

    Lane 1: SH-SY5Y lysates at 10 µg

    Lane 2: 293T lysate at 10 µg

    Lane 3: Human fetal brain lysates at 10 µg

    Lane 4: HepG2 lysates at 10 µg

    Lane 5: Seminoma lysates at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 41 kDa

  • OI-RD Scanning - Anti-GDF3 antibody [EPR4828] - BSA and Azide free (ab247699), expandable thumbnail

    OI-RD Scanning - Anti-GDF3 antibody [EPR4828] - BSA and Azide free (ab247699)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com