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AB201954

Anti-GDF8 / Myostatin antibody [6E4B2]

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(11 Publications)

Mouse Monoclonal GDF8 / Myostatin antibody. Suitable for Flow Cyt, WB, IHC-P and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MSTN aa 1-300.

View Alternative Names

GDF8, MSTN, Growth/differentiation factor 8, GDF-8, Myostatin

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF8 / Myostatin antibody [6E4B2] (AB201954)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF8 / Myostatin antibody [6E4B2] (AB201954)

Immunohistochemical analysis of Human liver cancer tissue labeling GDF8 / Myostatin using ab201954 at 1/200 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF8 / Myostatin antibody [6E4B2] (AB201954)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF8 / Myostatin antibody [6E4B2] (AB201954)

Immunohistochemical analysis of Human cervical cancer tissue labeling GDF8 / Myostatin using ab201954 at 1/200 dilution.

Flow Cytometry - Anti-GDF8 / Myostatin antibody [6E4B2] (AB201954)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-GDF8 / Myostatin antibody [6E4B2] (AB201954)

Flow cytometric analysis of LNcap cells labeling GDF8 / Myostatin using ab201954 at 1/200 dilution (green) and negative control (red).

Western blot - Anti-GDF8 / Myostatin antibody [6E4B2] (AB201954)
  • WB

Supplier Data

Western blot - Anti-GDF8 / Myostatin antibody [6E4B2] (AB201954)

All lanes:

Western blot - Anti-GDF8 / Myostatin antibody [6E4B2] (ab201954) at 1/500 dilution

All lanes:

LNcap cell lysate

Predicted band size: 43 kDa

false

Western blot - Anti-GDF8 / Myostatin antibody [6E4B2] (AB201954)
  • WB

Supplier Data

Western blot - Anti-GDF8 / Myostatin antibody [6E4B2] (AB201954)

Expected MW is 28.9 kDa

All lanes:

Western blot - Anti-GDF8 / Myostatin antibody [6E4B2] (ab201954) at 1/500 dilution

All lanes:

Human GDF8 / Myostatin (AA:24-266 ) recombinant protein

Predicted band size: 43 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

6E4B2

Isotype

IgG2b

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Human MSTN aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

O14793

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-ab170192'>ab170192</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chicken": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Goat": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Horse": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Sheep": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

This product was changed from ascites to supernatant. Lot no's high than GR285002-6 are from Tissue Culture Supernatant
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified from tissue culture supernatant.
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts specifically as a negative regulator of skeletal muscle growth.
See full target information MSTN
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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed38791317

2024

Inactivation of Myostatin Delays Senescence via TREX1-SASP in Bovine Skeletal Muscle Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Miaomiao Yang,Li Gao,Yajie Gao,Zhenting Hao,Xinyu Zhou,Guanghua Su,Chunling Bai,Zhuying Wei,Xuefei Liu,Lei Yang,Guangpeng Li

Recent patents on anti-cancer drug discovery 19:188-198 PubMed38214358

2024

Human Chorionic Gonadotropin Regulates the Smad Signaling Pathway by Antagonizing TGF-β in Giant Cell Tumor of Bone.

Applications

Unspecified application

Species

Unspecified reactive species

Tangbing Xu,Shenglin Xu,Guangwen Ma,Jun Chang,Chi Zhang,Ping Zhou,Chao Wang,Pengfei Xu,Junjun Yang,Yong Hu,Yunfeng Wu

EMBO reports 25:832-852 PubMed38191874

2024

BRD4 isoforms have distinct roles in tumour progression and metastasis in rhabdomyosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Dipanwita Das,Jia Yu Leung,Shivaranjani Balamurugan,Vinay Tergaonkar,Amos Hong Pheng Loh,Cheng-Ming Chiang,Reshma Taneja

Thoracic cancer 14:1969-1979 PubMed37220935

2023

Circular non-coding RNA circ_0072088 serves as a ceRNA, targeting the miR-1225-5p/WT1 axis to regulate non-small cell lung cancer cell malignant behavior.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaofang Zhu,Jing Wan,Xu You,Wanli Yang,Lei Zhao

International journal of molecular sciences 24: PubMed36901894

2023

Myostatin Overexpression and Smad Pathway in Detrusor Derived from Pediatric Patients with End-Stage Lower Urinary Tract Dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Souzan Salemi,Larissa J Schori,Tim Gerwinn,Maya Horst,Daniel Eberli

International journal of molecular sciences 23: PubMed36430183

2022

Myostatin Knockout Affects Mitochondrial Function by Inhibiting the AMPK/SIRT1/PGC1α Pathway in Skeletal Muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Mingjuan Gu,Zhuying Wei,Xueqiao Wang,Yang Gao,Dong Wang,Xuefei Liu,Chunling Bai,Guanghua Su,Lei Yang,Guangpeng Li

International journal of molecular sciences 23: PubMed36012334

2022

A New Method of Myostatin Inhibition in Mice via Oral Administration of Expressing Modified Myostatin Protein, BLS-M22.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Kyung Sung,Hyeongseop Kim,Sang Eon Park,Jiwon Lee,Ju-A Kim,Young-Chul Park,Hong Bae Jeon,Jong Wook Chang,Jeehun Lee

Oxidative medicine and cellular longevity 2022:3497644 PubMed35663205

2022

Myostatin Deficiency Enhances Antioxidant Capacity of Bovine Muscle via the SMAD-AMPK-G6PD Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Zhu,Xueqiao Wang,Zhuying Wei,Miaomiao Yang,Xinyu Zhou,Jiaru Lei,Chunling Bai,Guanghua Su,Xuefei Liu,Lei Yang,Guangpeng Li

Cells 11: PubMed35406665

2022

The Effect of Ex Vivo Human Serum from Liver Disease Patients on Cellular Protein Synthesis and Growth.

Applications

Unspecified application

Species

Unspecified reactive species

Sophie L Allen,Alex P Seabright,Jonathan I Quinlan,Amritpal Dhaliwal,Felicity R Williams,Nicholas H F Fine,David J Hodson,Matthew J Armstrong,Ahmed M Elsharkaway,Carolyn A Greig,Yu-Chiang Lai,Janet M Lord,Gareth G Lavery,Leigh Breen

Frontiers in cell and developmental biology 9:774185 PubMed35155444

2022

Myostatin Mutation Promotes Glycolysis by Increasing Phosphorylation of Phosphofructokinase Activation of PDE5A-cGMP-PKG in Cattle Heart.

Applications

Unspecified application

Species

Unspecified reactive species

Mingjuan Gu,Xinyu Zhou,Lin Zhu,Yajie Gao,Li Gao,Chunling Bai,Lei Yang,Guangpeng Li
View all publications
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