AB181213

Anti-GDI1 antibody [EPR13195]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal GDI1 antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

GDIL, OPHN2, RABGDIA, XAP4, GDI1, Rab GDP dissociation inhibitor alpha, Rab GDI alpha, Guanosine diphosphate dissociation inhibitor 1, Oligophrenin-2, Protein XAP-4, GDI-1

3 Images

Flow Cytometry (Intracellular) - Anti-GDI1 antibody [EPR13195] (AB181213)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-GDI1 antibody [EPR13195] (AB181213)

Intracellular flow cytometric analysis of HeLa cells (2% paraformaldehyde-fixed) labeling GDI1 with ab181213at 1/40 dilution (red), or a rabbit monoclonal IgG isotype control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as a secondary antibody.

Supplier Data

Immunoprecipitation - Anti-GDI1 antibody [EPR13195] (AB181213)

Western blot analysis of immunoprecipitation pellet from Human fetal brain lysate (lane 1) or a negative control (lane 2) immunoprecipitated using ab181213 at 1/50 dilution.
Secondary : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-GDI1 antibody [EPR13195] (ab181213)

Predicted band size: 51 kDa

false

Supplier Data

Western blot - Anti-GDI1 antibody [EPR13195] (AB181213)

All lanes:

Western blot - Anti-GDI1 antibody [EPR13195] (ab181213) at 1/10000 dilution

Lane 1:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Lane 3:

SH-SY5Y cell lysate at 20 µg

Lane 4:

Human fetal brain tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 51 kDa

false

Carrier free

Anti-GDI1 antibody [EPR13195] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13195

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30 - 1/50", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/40", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Regulates the GDP/GTP exchange reaction of most Rab proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. Promotes the dissociation of GDP-bound Rab proteins from the membrane and inhibits their activation. Promotes the dissociation of RAB1A, RAB3A, RAB5A and RAB10 from membranes.

See full target information GDI1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:7421 PubMed39198433

2024

IGF2BP3 promotes mRNA degradation through internal mG modification.

Applications

Unspecified application

Species

Unspecified reactive species

Chang Liu,Xiaoyang Dou,Yutao Zhao,Linda Zhang,Lisheng Zhang,Qing Dai,Jun Liu,Tong Wu,Yu Xiao,Chuan He

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GDI1 antibody [EPR13195]

Flow Cytometry (Intracellular) - Anti-GDI1 antibody [EPR13195] (AB181213)

Immunoprecipitation - Anti-GDI1 antibody [EPR13195] (AB181213)

Western blot - Anti-GDI1 antibody [EPR13195] (AB181213)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

Product promise