Rabbit Recombinant Monoclonal GDI1 antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples.
Images
![Western blot - Anti-GDI1 antibody [EPR13195] - BSA and Azide free (AB250432), expandable thumbnail](https://content.abcam.com/products/images/gdi1-antibody-epr13195-bsa-and-azide-free-ab250432--western-blot-img104809.png/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GDI1 antibody [EPR13195] - BSA and Azide free (AB250432)")
![Immunoprecipitation - Anti-GDI1 antibody [EPR13195] - BSA and Azide free (AB250432), expandable thumbnail](https://content.abcam.com/products/images/gdi1-antibody-epr13195-bsa-and-azide-free-ab250432--immunoprecipitation-img62660.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-GDI1 antibody [EPR13195] - BSA and Azide free (AB250432)")
![Flow Cytometry (Intracellular) - Anti-GDI1 antibody [EPR13195] - BSA and Azide free (AB250432), expandable thumbnail](https://content.abcam.com/products/images/gdi1-antibody-epr13195-bsa-and-azide-free-ab250432--flow-cytometry-intracellular-img50910.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-GDI1 antibody [EPR13195] - BSA and Azide free (AB250432)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | Flow Cyt (Intra) | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Regulates the GDP/GTP exchange reaction of most Rab proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. Promotes the dissociation of GDP-bound Rab proteins from the membrane and inhibits their activation. Promotes the dissociation of RAB1A, RAB3A, RAB5A and RAB10 from membranes.
Alternative names
GDIL, OPHN2, RABGDIA, XAP4, GDI1, Rab GDP dissociation inhibitor alpha, Rab GDI alpha, Guanosine diphosphate dissociation inhibitor 1, Oligophrenin-2, Protein XAP-4, GDI-1
Recommended products
Rabbit Recombinant Monoclonal GDI1 antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR13195
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab250432 is the carrier-free version of Anti-GDI1 antibody [EPR13195] ab181213.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-GDI1 antibody [EPR13195] - BSA and Azide free (ab250432), expandable thumbnail](https://content.abcam.com/products/images/gdi1-antibody-epr13195-bsa-and-azide-free-ab250432--western-blot-img104809.png "Western blot - Anti-GDI1 antibody [EPR13195] - BSA and Azide free (ab250432)")
Western blot - Anti-GDI1 antibody [EPR13195] - BSA and Azide free (ab250432)
This data was developed using Anti-GDI1 antibody [EPR13195] ab181213, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-GDI1 antibody [EPR13195] (Anti-GDI1 antibody [EPR13195] ab181213) at 1/10000 dilution
Lane 1: U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate at 20 µg
Lane 2: HeLa cell lysate at 20 µg
Lane 3: SH-SY5Y cell lysate at 20 µg
Lane 4: Human fetal brain tissue lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 51 kDa
![Immunoprecipitation - Anti-GDI1 antibody [EPR13195] - BSA and Azide free (ab250432), expandable thumbnail](https://content.abcam.com/products/images/gdi1-antibody-epr13195-bsa-and-azide-free-ab250432--immunoprecipitation-img62660.jpg "Immunoprecipitation - Anti-GDI1 antibody [EPR13195] - BSA and Azide free (ab250432)")
Immunoprecipitation - Anti-GDI1 antibody [EPR13195] - BSA and Azide free (ab250432)
This data was developed using Anti-GDI1 antibody [EPR13195] ab181213, the same antibody clone in a different buffer formulation.Western blot analysis of immunoprecipitation pellet from Human fetal brain lysate (lane 1) or a negative control (lane 2) immunoprecipitated using Anti-GDI1 antibody [EPR13195] ab181213 at 1/50 dilution.
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.All lanes: Immunoprecipitation - Anti-GDI1 antibody [EPR13195] (Anti-GDI1 antibody [EPR13195] ab181213)
Predicted band size: 51 kDa
![Flow Cytometry (Intracellular) - Anti-GDI1 antibody [EPR13195] - BSA and Azide free (ab250432), expandable thumbnail](https://content.abcam.com/products/images/gdi1-antibody-epr13195-bsa-and-azide-free-ab250432--flow-cytometry-intracellular-img50910.jpg "Flow Cytometry (Intracellular) - Anti-GDI1 antibody [EPR13195] - BSA and Azide free (ab250432)")
Flow Cytometry (Intracellular) - Anti-GDI1 antibody [EPR13195] - BSA and Azide free (ab250432)
This data was developed using Anti-GDI1 antibody [EPR13195] ab181213, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of HeLa cells (2% paraformaldehyde-fixed) labeling GDI1 with Anti-GDI1 antibody [EPR13195] ab181213 at 1/40 dilution (red), or a rabbit monoclonal IgG isotype control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as a secondary antibody.
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