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AB244211

Anti-GDNF antibody [BS376]

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(3 Publications)

Mouse Monoclonal GDNF antibody. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Rat, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human GDNF aa 50-100.

View Alternative Names

Glial cell line-derived neurotrophic factor, hGDNF, Astrocyte-derived trophic factor, ATF, GDNF

3 Images
Flow Cytometry (Intracellular) - Anti-GDNF antibody [BS376] (AB244211)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-GDNF antibody [BS376] (AB244211)

Fixing and permeabilization of SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells : Absolute methanol (10 minutes in ice) and 0.1% Tween-20 in PBS, Blocking : 1% BSA, Primary antibody : ab244211 at 2 μg per ~106 cells for 30 minutes at room temperature, Secondary antibody : Goat anti-mouse PE labeled secondary antibody (1/100) with incubation for 20 minutes in dark at room temperature. Non-specific Control IgG, clone X63 was used as negative control under same conditions (black dashed).

Immunocytochemistry/ Immunofluorescence - Anti-GDNF antibody [BS376] (AB244211)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GDNF antibody [BS376] (AB244211)

Immunofluorescence analysis of proGDNF expression in SH-SY5Y (human neuroblastoma cell line from bone marrow) cells. Fixed (4% formaldehyde), permeabilized, and blocked (10% normal horse serum, 0.1% Triton X-100) SH-SY5Y cells were incubated with ab244211 at 2 μg/mL, (green) for 1 hour. Primary antibody binding was visualized with a secondary donkey anti-mouse-CF488A antibody (4 μg/mL, 1 hour incubation). Cell nuclei were stained with Hoechst dye (blue).

Immunocytochemistry/ Immunofluorescence - Anti-GDNF antibody [BS376] (AB244211)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GDNF antibody [BS376] (AB244211)

Immunofluorescence analysis of proGDNF expression in C6 (rat glial tumor cell line) cells. Fixed (4% formaldehyde), permeabilized, and blocked (10% normal horse serum, 0.1% Triton X-100) SH-SY5Y cells were incubated with ab244211 at 2 μg/mL, (green) for 1 hour. Primary antibody binding was visualized with a secondary donkey anti-mouse-CF488A antibody (4 μg/mL, 1 hour incubation). Cell nuclei were stained with Hoechst dye (blue).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

BS376

Isotype

IgG3

Light chain type

kappa

Carrier free

No

Reacts with

Rat, Human

Applications

Flow Cyt (Intra), ICC/IF

applications

Immunogen

Synthetic Peptide within Human GDNF aa 50-100. The exact immunogen used to generate this antibody is proprietary information.

P39905

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-2 µg/mL", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "2 µg/cells for 6 µg/cells", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-2 µg/mL", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Properties and storage information

Form
Lyophilized
Reconstitution
reconstitute with water at 100µL
Purification technique
Affinity purification Protein G
Purification notes
Purified from TCS.
Storage buffer
pH: 7 - 7.4 Constituents: PBS, 3% Trehalose
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake (PubMed : 8493557). Acts by binding to its coreceptor, GFRA1, leading to autophosphorylation and activation of the RET receptor (PubMed : 10829012, PubMed : 25242331, PubMed : 31535977). Involved in the development of the neural crest (PubMed : 15242795).
See full target information GDNF
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 10:e38070 PubMed39381248

2024

Neuroprotective effects of pink lotus oil in kainic acid-induced epilepsy.

Applications

Unspecified application

Species

Unspecified reactive species

Ratchaniporn Kongsui,Teera Chanmanee,Tichanon Promsrisuk,Lars Klimaschewski,Napatr Sriraksa,Jinatta Jittiwat,Sitthisak Thongrong

Molecular and cellular biology 44:87-102 PubMed38520226

2024

LncRNA AA465934 Improves Podocyte Injury by Promoting Tristetraprolin-Mediated HMGB1 DownRegulation in Diabetic Nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Nan Yang,Yue Zhang,Peiyao Ren,Li Zhao,Danna Zheng,Lanjun Fu,Juan Jin

F1000Research 12:846 PubMed38434672

2024

Pinostrobin mitigates neurodegeneration through an up-regulation of antioxidants and GDNF in a rat model of Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Ratchaniporn Kongsui,Tichanon Promsrisuk,Lars Klimaschewski,Napatr Sriraksa,Jinatta Jittiwat,Sitthisak Thongrong
View all publications
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