Anti-GEF H1 antibody

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GEF H1 antibody (AB155785)

Immunofluorescence analysis of HeLa cells fixed in ice-cold MeOH for 5 min labelling GEF-H1 protein at cytoskeleton and Golgi apparatus using ab155785 at a 1/500 dilution (Green). Hoechst 33342 was used for nuclear staining (Blue).
Scale bar = 10 µM

• ICC/IF

AbReview51054****

Immunocytochemistry/ Immunofluorescence - Anti-GEF H1 antibody (AB155785)

Immunocytochemical immunofluorescence analysis of formaldehyde-fixed human HepG2 cells, labelling GEF H1 with ab155785 with a dilution of 1/50 incubated for 3 hours at 22°C in 1% donkey serum in 0.1% PBST diluent. Permeablization with 0.2% Triton X-100. Blocking was with 1% donkey serum in 0.1% PBST incubated for 1 hour at 22°C. Secondary was a donkey anti-rabbit polyclonal Alexa Fluor^® 594 at 1/200. Counterstain is DAPI (blue) against nuclear DNA.

Image is courtesy of an AbReview submitted by Dr Armen Petrosyan.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GEF H1 antibody (AB155785)

Immunohistochemical analysis of paraffin-embedded Human Gastric carcinoma tissue, labeling GEF H1 with ab155785 at 1/500 dilution.

• IP

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Immunoprecipitation - Anti-GEF H1 antibody (AB155785)

Immunoprecipitation of GEF H1 from 293T whole cell extracts using 5 μg of ab155785. Western blot analysis was performed using ab155785.

All lanes:

Immunoprecipitation - Anti-GEF H1 antibody (ab155785)

Predicted band size: 111 kDa

false

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GEF H1 antibody (AB155785)

Immunohistochemical analysis of paraffin-embedded mouse brain tissue stained for GEF-H1 protein at cytoplasm with ab155785 at a 1/1000 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GEF H1 antibody (AB155785)

Immunohistochemical analysis of paraffin-embedded Mouse testis tissue, labeling GEF H1 with ab155785 at 1/500 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GEF H1 antibody (AB155785)

Immunohistochemical analysis of paraffin-embedded rat hind brain tissue stained for GEF-H1 protein at cytosol on rat hind brain with ab155785 at a 1/500 dilution.

Antigen retrieval : Trilogy™ (EDTA based, pH 8.0) buffer, 15min

• WB

Supplier Data

Western blot - Anti-GEF H1 antibody (AB155785)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-GEF H1 antibody (ab155785) at 1/2000 dilution

Lane 1:

293T whole cell extracts at 30 µg

Lane 2:

A431 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 20 kDa

false

• WB

Supplier Data

Western blot - Anti-GEF H1 antibody (AB155785)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-GEF H1 antibody (ab155785) at 1/4000 dilution

Lane 1:

293T whole cell extracts at 30 µg

Lane 2:

shRNA transfected 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 111 kDa

false

• WB

Supplier Data

Western blot - Anti-GEF H1 antibody (AB155785)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-GEF H1 antibody (ab155785) at 1/1000 dilution

Lane 1:

Neuro2A whole cell lysate at 30 µg

Lane 2:

GL261 whole cell lysate at 30 µg

Lane 3:

C8D30 whole cell lysate at 30 µg

Lane 4:

NIH-3T3 whole cell lysate at 30 µg

Lane 5:

BCL-1 whole cell lysate at 30 µg

Lane 6:

Raw 264.7 whole cell lysate at 30 µg

Lane 7:

C2Cl2 whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 111 kDa

false

• WB

Supplier Data

Western blot - Anti-GEF H1 antibody (AB155785)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-GEF H1 antibody (ab155785) at 1/500 dilution

Lane 1:

PC-12 whole cell extracts at 30 µg

Lane 2:

Rat2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 111 kDa

false

• WB

Supplier Data

Western blot - Anti-GEF H1 antibody (AB155785)

8% SDS PAGE

All lanes:

Western blot - Anti-GEF H1 antibody (ab155785) at 1/1000 dilution

Lane 1:

hMSC-3A6 whole cell lysate, mock transfected at 10 µg

Lane 2:

hMSC-3A6 whole cell lysate, transfected with GEF H1 siRNA at 10 µg

Predicted band size: 111 kDa

false