Rabbit Polyclonal GEF H1 antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 26 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ARHGEF2 aa 650 to C-terminus.
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- iScience 26:1080492023Endothelial barrier dysfunction in systemic inflammation is mediated by soluble VE-cadherin interfering VE-PTP signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJuna-Lisa Knop,Natalie Burkard,Mahshid Danesh,Sebastian Kintrup,Thomas Dandekar,Mugdha Srivastava,Rebecca Springer,Matthias Hiermaier,Nana-Maria Wagner,Jens Waschke,Sven Flemming,Nicolas SchlegelPubMed 37822505
- Journal of cell science 136:2023Lfc subcellular localization and activity is controlled by αv-class integrin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGeorgina P Coló,Andrea Seiwert,Raquel B HagaPubMed 37129180
- eLife 12:2023Nuclear SUN1 stabilizes endothelial cell junctions via microtubules to regulate blood vessel formation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDanielle B Buglak,Pauline Bougaran,Molly R Kulikauskas,Ziqing Liu,Elizabeth Monaghan-Benson,Ariel L Gold,Allison P Marvin,Andrew Burciu,Natalie T Tanke,Morgan Oatley,Shea N Ricketts,Karina Kinghorn,Bryan N Johnson,Celia E Shiau,Stephen Rogers,Christophe Guilluy,Victoria L BautchPubMed 36989130
- Cells 11:2022Autophagy Induced by Toll-like Receptor Ligands Regulates Antigen Extraction and Presentation by B Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJonathan Lagos,Sara Sagadiev,Jheimmy Diaz,Juan Pablo Bozo,Fanny Guzman,Caroline Stefani,Silvana Zanlungo,Mridu Acharya,Maria Isabel YuseffPubMed 36497137
- Haematologica 107:2972-29762022Structure-function analysis of the role of megakaryoblastic leukemia 1 in megakaryocyte polyploidization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFiona E Reed,Nicole M Eskow,Elizabeth Min,Maximillian Carlino,Rubia Mancuso,Nayoung Kwon,Elenoe C Smith,Shannon T Larsuel,Lin Wang,Vanessa Scanlon,Diane S KrausePubMed 36453520
- Cell death & disease 13:9272022Androgen deprivation restores ARHGEF2 to promote neuroendocrine differentiation of prostate cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuanrong Chen,Yi Shao,Wanqing Wei,Shimiao Zhu,Yang Li,Yutong Chen,Hanling Li,Hao Tian,Guijiang Sun,Yuanjie Niu,Zhiqun ShangPubMed 36335093
- Scientific reports 12:173182022Lateral confined growth of cells activates Lef1 dependent pathways to regulate cell-state transitions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLuezhen Yuan,Bibhas Roy,Prasuna Ratna,Caroline Uhler,G V ShivashankarPubMed 36243826
- Journal of cell science 135:2022Molecular determinants of αVβ5 localization in flat clathrin lattices - role of αVβ5 in cell adhesion and proliferation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlba Zuidema,Wei Wang,Maaike Kreft,Onno B Bleijerveld,Liesbeth Hoekman,Jonas Aretz,Ralph T Böttcher,Reinhard Fässler,Arnoud SonnenbergPubMed 35532004
- The Journal of cell biology 221:2022Interphase microtubule disassembly is a signaling cue that drives cell rounding at mitotic entry.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKévin Leguay,Barbara Decelle,Islam E Elkholi,Michel Bouvier,Jean-François Côté,Sébastien CarrénoPubMed 35482006
- Nature materials 21:366-3772021Microtubules tune mechanosensitive cell responses.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShailaja Seetharaman,Benoit Vianay,Vanessa Roca,Aaron J Farrugia,Chiara De Pascalis,Batiste Boëda,Florent Dingli,Damarys Loew,Stéphane Vassilopoulos,Alexander Bershadsky,Manuel Théry,Sandrine Etienne-MannevillePubMed 34663953
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ARHGEF2 aa 650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q92974
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Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Expected | Tested | Tested | Expected |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/3000 | Notes - |
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 - 1/1000 | Notes - |
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
Activates Rho-GTPases by promoting the exchange of GDP for GTP. May be involved in epithelial barrier permeability, cell motility and polarization, dendritic spine morphology, antigen presentation, leukemic cell differentiation, cell cycle regulation, innate immune response, and cancer. Binds Rac-GTPases, but does not seem to promote nucleotide exchange activity toward Rac-GTPases, which was uniquely reported in PubMed:9857026. May stimulate instead the cortical activity of Rac. Inactive toward CDC42, TC10, or Ras-GTPases. Forms an intracellular sensing system along with NOD1 for the detection of microbial effectors during cell invasion by pathogens. Required for RHOA and RIP2 dependent NF-kappaB signaling pathways activation upon S.flexneri cell invasion. Involved not only in sensing peptidoglycan (PGN)-derived muropeptides through NOD1 that is independent of its GEF activity, but also in the activation of NF-kappaB by Shigella effector proteins (IpgB2 and OspB) which requires its GEF activity and the activation of RhoA. Involved in innate immune signaling transduction pathway promoting cytokine IL6/interleukin-6 and TNF-alpha secretion in macrophage upon stimulation by bacterial peptidoglycans; acts as a signaling intermediate between NOD2 receptor and RIPK2 kinase. Contributes to the tyrosine phosphorylation of RIPK2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Overexpression activates Rho-, but not Rac-GTPases, and increases paracellular permeability (By similarity). Involved in neuronal progenitor cell division and differentiation (PubMed:28453519). Involved in the migration of precerebellar neurons (By similarity).
Alternative names
KIAA0651, LFP40, ARHGEF2, Rho guanine nucleotide exchange factor 2, Guanine nucleotide exchange factor H1, Microtubule-regulated Rho-GEF, Proliferating cell nucleolar antigen p40, GEF-H1
Recommended products
Rabbit Polyclonal GEF H1 antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 26 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ARHGEF2 aa 650 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ARHGEF2 aa 650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q92974
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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8 product images
Western blot - Anti-GEF H1 antibody (ab155785)
5% SDS PAGE
All lanes: Western blot - Anti-GEF H1 antibody (ab155785) at 1/2000 dilution
Lane 1: 293T whole cell lysate at 30 µg
Lane 2: A431 whole cell lysate at 30 µg
Lane 3: HeLa whole cell lysate at 30 µg
Predicted band size: 111 kDa
Immunoprecipitation - Anti-GEF H1 antibody (ab155785)
Immunoprecipitation of GEF H1 from 293T whole cell extracts using 5 μg of ab155785. Western blot analysis was performed using ab155785.
All lanes: Immunoprecipitation - Anti-GEF H1 antibody (ab155785)
Predicted band size: 111 kDa
Image is courtesy of a customer review submitted by Dr Armen Petrosyan.Immunocytochemistry/ Immunofluorescence - Anti-GEF H1 antibody (ab155785)
Immunocytochemical immunofluorescence analysis of formaldehyde-fixed human HepG2 cells, labelling GEF H1 with ab155785 with a dilution of 1/50 incubated for 3 hours at 22°C in 1% donkey serum in 0.1% PBST diluent. Permeablization with 0.2% Triton X-100. Blocking was with 1% donkey serum in 0.1% PBST incubated for 1 hour at 22°C. Secondary was a donkey anti-rabbit polyclonal Alexa Fluor® 594 at 1/200. Counterstain is DAPI (blue) against nuclear DNA.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GEF H1 antibody (ab155785)
Immunohistochemical analysis of paraffin-embedded Human Gastric carcinoma tissue, labeling GEF H1 with ab155785 at 1/500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GEF H1 antibody (ab155785)
Immunohistochemical analysis of paraffin-embedded Mouse testis tissue, labeling GEF H1 with ab155785 at 1/500 dilution.
Western blot - Anti-GEF H1 antibody (ab155785)
8% SDS PAGE
All lanes: Western blot - Anti-GEF H1 antibody (ab155785) at 1/1000 dilution
Lane 1: hMSC-3A6 whole cell lysate, mock transfected at 10 µg
Lane 2: hMSC-3A6 whole cell lysate, transfected with GEF H1 siRNA at 10 µg
Predicted band size: 111 kDa
Western blot - Anti-GEF H1 antibody (ab155785)
5 % SDS-PAGE
All lanes: Western blot - Anti-GEF H1 antibody (ab155785) at 1/1000 dilution
Lane 1: RAW264.7 whole cell lysate at 30 µg
Lane 2: C2C12 whole cell lysate at 30 µg
Predicted band size: 111 kDa
Immunocytochemistry/ Immunofluorescence - Anti-GEF H1 antibody (ab155785)
Immunofluorescent analysis of methanol-fixed (-20°C 100% MeOH for 5 min) HeLa cells, labeling GEF H1 with ab155785 at 1/500 dilution. Right image shows cells co-stained with Hoechst 33342.
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