AB90783

Anti-GEF H1 antibody [B4/7]

Name: Anti-GEF H1 antibody [B4/7] (ab90783) | Abcam
Brand: Abcam Limited
SKU: AB90783
Price: 505 USD
Availability: InStock
Rating: 4 (3 reviews)

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What is this?

(3 Reviews)

(7 Publications)

Mouse Monoclonal GEF H1 antibody. Suitable for Flow Cyt, IHC-P and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Cell preparation containing ARHGEF2 protein.

View Alternative Names

Rho guanine nucleotide exchange factor 2, Guanine nucleotide exchange factor H1, Tight junction-associated guanine nucleotide exchange factor isoform A, GEF-H1

2 Images

Flow Cytometry - Anti-GEF H1 antibody [B4/7] (AB90783)

Flow Cyt

Unknown

Flow Cytometry - Anti-GEF H1 antibody [B4/7] (AB90783)

Overlay histogram showing HeLa cells stained with ab90783 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab90783, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GEF H1 antibody [B4/7] (AB90783)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GEF H1 antibody [B4/7] (AB90783)

IHC image of ab90783 staining in human breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab90783, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

B4/7

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

Flow Cyt, IHC-P

applications

Immunogen

Cell preparation containing ARHGEF2 protein. The exact immunogen used to generate this antibody is proprietary information.

Q865S3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-2 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p>" }, "Dog": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

0.2 µm filtered antibody solution

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Activates Rho-GTPases by promoting the exchange of GDP for GTP. May be involved in epithelial barrier permeability, cell motility and polarization, dendritic spine morphology, antigen presentation, leukemic cell differentiation, cell cycle regulation, innate immune response, and cancer. Binds Rac-GTPases, but does not seem to promote nucleotide exchange activity toward Rac-GTPases. May stimulate instead the cortical activity of Rac. Inactive toward CDC42, TC10, or Ras-GTPases. Forms an intracellular sensing system along with NOD1 for the detection of microbial effectors during cell invasion by pathogens. Involved in innate immune signaling transduction pathway promoting cytokine IL6/interleukin-6 and TNF secretion in macrophage upon stimulation by bacterial peptidoglycans; acts as a signaling intermediate between NOD2 receptor and RIPK2 kinase. Contributes to the tyrosine phosphorylation of RIPK2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Involved in neuronal progenitor cell division and differentiation. Involved in the migration of precerebellar neurons (By similarity). Overexpression activates Rho-, but not Rac-GTPases, and increases paracellular permeability (PubMed : 12604587).

See full target information ARHGEF2

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:1394 PubMed39952917

2025

Matrix mechano-sensing at the invasive front induces a cytoskeletal and transcriptional memory supporting metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Oscar Maiques,Marta C Sallan,Roman Laddach,Pahini Pandya,Adrian Varela,Eva Crosas-Molist,Jaume Barcelo,Olivia Courbot,Yanbo Liu,Vittoria Graziani,Youssef Arafat,Joanne Sewell,Irene Rodriguez-Hernandez,Bruce Fanshawe,Yaiza Jung-Garcia,Paul Rc Imbert,Eloise M Grasset,Jean Albrengues,Maria Santacana,Anna Macià,Jordi Tarragona,Xavier Matias-Guiu,Rosa M Marti,Sophia Tsoka,Cedric Gaggioli,Jose L Orgaz,Gilbert O Fruhwirth,Fredrik Wallberg,Kai Betteridge,Constantino Carlos Reyes-Aldasoro,Syed Haider,Andrejs Braun,Sophia N Karagiannis,Alberto Elosegui-Artola,Victoria Sanz-Moreno

The Journal of cell biology 218:1725-1742 PubMed30948425

2019

VE-PTP stabilizes VE-cadherin junctions and the endothelial barrier via a phosphatase-independent mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Vanessa V Juettner,Kevin Kruse,Arkaprava Dan,Vinh H Vu,Yousaf Khan,Jonathan Le,Deborah Leckband,Yulia Komarova,Asrar B Malik

Pathology, research and practice 215:1012-1019 PubMed30846413

2019

Increased expression of GEF-H1 promotes colon cancer progression by RhoA signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Cao,Tian Yang,DeHua Tang,Fan Zhou,YuanXia Qian,XiaoPing Zou

Biochimica et biophysica acta. Molecular cell rese 1864:1714-1733 PubMed28554775

2017

Zonula occludens-2 regulates Rho proteins activity and the development of epithelial cytoarchitecture and barrier function.

Applications

Unspecified application

Species

Unspecified reactive species

Arturo Raya-Sandino,Alejandro Castillo-Kauil,Alaide Domínguez-Calderón,Lourdes Alarcón,David Flores-Benitez,Francisco Cuellar-Perez,Bruno López-Bayghen,Bibiana Chávez-Munguía,José Vázquez-Prado,Lorenza González-Mariscal

The Journal of clinical investigation 126:585-98 PubMed26690702

2015

Wnt5a induces ROR1/ROR2 heterooligomerization to enhance leukemia chemotaxis and proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Yu,Liguang Chen,Bing Cui,George F Widhopf,Zhouxin Shen,Rongrong Wu,Ling Zhang,Suping Zhang,Steven P Briggs,Thomas J Kipps

The Journal of biological chemistry 289:22500-11 PubMed24986862

2014

ZO proteins redundantly regulate the transcription factor DbpA/ZONAB.

Applications

Unspecified application

Species

Unspecified reactive species

Domenica Spadaro,Rocio Tapia,Lionel Jond,Marius Sudol,Alan S Fanning,Sandra Citi

Journal of cell science 124:1214-23 PubMed21406564

2011

Calpain-6, a microtubule-stabilizing protein, regulates Rac1 activity and cell motility through interaction with GEF-H1.

Applications

WB, ICC/IF

Species

Mouse, Mouse

Kazuo Tonami,Yukiko Kurihara,Satoshi Arima,Koichi Nishiyama,Yasunobu Uchijima,Tomoichiro Asano,Hiroyuki Sorimachi,Hiroki Kurihara

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GEF H1 antibody [B4/7]

Flow Cytometry - Anti-GEF H1 antibody [B4/7] (AB90783)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GEF H1 antibody [B4/7] (AB90783)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (7)

Product promise