Rabbit Recombinant Monoclonal GEF H1 antibody. C-terminal. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Human, Rat samples. Cited in 4 publications.
Images
![Western blot - Anti-GEF H1 antibody [EPR17963] - C-terminal (AB201687), expandable thumbnail](https://content.abcam.com/products/images/gef-h1-antibody-epr17963-c-terminal-ab201687--western-blot-img100359.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GEF H1 antibody [EPR17963] - C-terminal (AB201687)")
![Western blot - Anti-GEF H1 antibody [EPR17963] - C-terminal (AB201687), expandable thumbnail](https://content.abcam.com/products/images/gef-h1-antibody-epr17963-c-terminal-ab201687--western-blot-img10506.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GEF H1 antibody [EPR17963] - C-terminal (AB201687)")
![Western blot - Anti-GEF H1 antibody [EPR17963] - C-terminal (AB201687), expandable thumbnail](https://content.abcam.com/products/images/gef-h1-antibody-epr17963-c-terminal-ab201687--western-blot-img160083.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GEF H1 antibody [EPR17963] - C-terminal (AB201687)")
![Western blot - Anti-GEF H1 antibody [EPR17963] - C-terminal (AB201687), expandable thumbnail](https://content.abcam.com/products/images/gef-h1-antibody-epr17963-c-terminal-ab201687--western-blot-img108217.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GEF H1 antibody [EPR17963] - C-terminal (AB201687)")
![Immunocytochemistry/ Immunofluorescence - Anti-GEF H1 antibody [EPR17963] - C-terminal (AB201687), expandable thumbnail](https://content.abcam.com/products/images/gef-h1-antibody-epr17963-c-terminal-ab201687--immunocytochemistry-immunofluorescence-img111299.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-GEF H1 antibody [EPR17963] - C-terminal (AB201687)")
Publications
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- Evidence-based complementary and alternative medicine : eCAM 2022:70741572022Catalpol Regulates Oligodendrocyte Regeneration and Remyelination by Activating the GEF-Cdc42/Rac1 Signaling Pathway in EAE Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMinghui Wu,Qi Kang,Yuezhi Kang,Yanping Tong,Tao Yang,Yongping FanPubMed 36482934
- Cell death & disease 13:9272022Androgen deprivation restores ARHGEF2 to promote neuroendocrine differentiation of prostate cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuanrong Chen,Yi Shao,Wanqing Wei,Shimiao Zhu,Yang Li,Yutong Chen,Hanling Li,Hao Tian,Guijiang Sun,Yuanjie Niu,Zhiqun ShangPubMed 36335093
- Blood advances 5:3120-31332021Arhgef2 regulates mitotic spindle orientation in hematopoietic stem cells and is essential for productive hematopoiesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDerek C H Chan,Joshua Xu,Ana Vujovic,Nicholas Wong,Victor Gordon,Laura P M H de Rooij,Steven Moreira,Cailin E Joyce,Jose La Rose,María-José Sandí,Bradley W Doble,Carl D Novina,Robert K Rottapel,Kristin J HopePubMed 34406376
- Annals of translational medicine 9:1652021Actin-granule formation is an additional step in cardiac myofibroblast differentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi He,Ruiqi Liu,Honghua Yue,Shuofang Ren,Guonian Zhu,Yingqiang Guo,Chaoyi QinPubMed 33569467
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected |
| Rat | Tested | Expected | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/2000 | Notes - |
Species Human | Dilution info 1/2000 | Notes - |
Species Rat | Dilution info 1/2000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 | Notes - |
Species Rat | Dilution info 1/500 | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Activates Rho-GTPases by promoting the exchange of GDP for GTP. May be involved in epithelial barrier permeability, cell motility and polarization, dendritic spine morphology, antigen presentation, leukemic cell differentiation, cell cycle regulation, innate immune response, and cancer. Binds Rac-GTPases, but does not seem to promote nucleotide exchange activity toward Rac-GTPases, which was uniquely reported in PubMed:9857026. May stimulate instead the cortical activity of Rac. Inactive toward CDC42, TC10, or Ras-GTPases. Forms an intracellular sensing system along with NOD1 for the detection of microbial effectors during cell invasion by pathogens. Required for RHOA and RIP2 dependent NF-kappaB signaling pathways activation upon S.flexneri cell invasion. Involved not only in sensing peptidoglycan (PGN)-derived muropeptides through NOD1 that is independent of its GEF activity, but also in the activation of NF-kappaB by Shigella effector proteins (IpgB2 and OspB) which requires its GEF activity and the activation of RhoA. Involved in innate immune signaling transduction pathway promoting cytokine IL6/interleukin-6 and TNF-alpha secretion in macrophage upon stimulation by bacterial peptidoglycans; acts as a signaling intermediate between NOD2 receptor and RIPK2 kinase. Contributes to the tyrosine phosphorylation of RIPK2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Overexpression activates Rho-, but not Rac-GTPases, and increases paracellular permeability (By similarity). Involved in neuronal progenitor cell division and differentiation (PubMed:28453519). Involved in the migration of precerebellar neurons (By similarity).
Alternative names
KIAA0651, LFP40, ARHGEF2, Rho guanine nucleotide exchange factor 2, Guanine nucleotide exchange factor H1, Microtubule-regulated Rho-GEF, Proliferating cell nucleolar antigen p40, GEF-H1
Recommended products
Rabbit Recombinant Monoclonal GEF H1 antibody. C-terminal. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Human, Rat samples. Cited in 4 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR17963
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Western blot - Anti-GEF H1 antibody [EPR17963] - C-terminal (ab201687), expandable thumbnail](https://content.abcam.com/products/images/gef-h1-antibody-epr17963-c-terminal-ab201687--western-blot-img100359.jpg "Western blot - Anti-GEF H1 antibody [EPR17963] - C-terminal (ab201687)")
Western blot - Anti-GEF H1 antibody [EPR17963] - C-terminal (ab201687)
Lanes 1-3: Merged signal (red and green). Green - ab201687 observed at 75 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.ab201687 Anti-GEF H1 antibody [EPR17963] - C-terminal was shown to specifically react with GEF H1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human ARHGEF2 (GEF H1) knockout HeLa cell line ab265797 (knockout cell lysate Human ARHGEF2 (GEF H1) knockout HeLa cell lysate ab257223) was used. Wild-type and GEF H1 knockout samples were subjected to SDS-PAGE. ab201687 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-GEF H1 antibody [EPR17963] - C-terminal (ab201687) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: ARHGEF2 knockout HeLa cell lysate at 20 µg
Lane 3: HEK293 cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 111 kDa
Observed band size: 75 kDa
![Western blot - Anti-GEF H1 antibody [EPR17963] - C-terminal (ab201687), expandable thumbnail](https://content.abcam.com/products/images/gef-h1-antibody-epr17963-c-terminal-ab201687--western-blot-img10506.jpg "Western blot - Anti-GEF H1 antibody [EPR17963] - C-terminal (ab201687)")
Western blot - Anti-GEF H1 antibody [EPR17963] - C-terminal (ab201687)
Lanes 1-3: Merged signal (red and green). Green - ab201687 observed at 112 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.ab201687 Anti-GEF H1 antibody [EPR17963] - C-terminal was shown to specifically react with GEF H1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human ARHGEF2 (GEF H1) knockout HeLa cell line ab265797 (knockout cell lysate Human ARHGEF2 (GEF H1) knockout HeLa cell lysate ab257223) was used. Wild-type and GEF H1 knockout samples were subjected to SDS-PAGE. ab201687 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-GEF H1 antibody [EPR17963] - C-terminal (ab201687) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: ARHGEF knockout HeLa cell lysate at 20 µg
Lane 2: Western blot - Human ARHGEF2 (GEF H1) knockout HeLa cell line (Human ARHGEF2 (GEF H1) knockout HeLa cell line ab265797)
Lane 3: HEK-293T cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 111 kDa
Observed band size: 112 kDa, 75 kDa
![Western blot - Anti-GEF H1 antibody [EPR17963] - C-terminal (ab201687), expandable thumbnail](https://content.abcam.com/products/images/gef-h1-antibody-epr17963-c-terminal-ab201687--western-blot-img160083.jpg "Western blot - Anti-GEF H1 antibody [EPR17963] - C-terminal (ab201687)")
Western blot - Anti-GEF H1 antibody [EPR17963] - C-terminal (ab201687)
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-GEF H1 antibody [EPR17963] - C-terminal (ab201687) at 1/10000 dilution
Lane 1: HEK293 (Human embryonic kidney) lysate at 20 µg
Lane 2: HEK293 (Human epithelial cells from cervix adenocarcinoma) lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 111 kDa
Observed band size: 112 kDa
Exposure time: 30s
![Western blot - Anti-GEF H1 antibody [EPR17963] - C-terminal (ab201687), expandable thumbnail](https://content.abcam.com/products/images/gef-h1-antibody-epr17963-c-terminal-ab201687--western-blot-img108217.jpg "Western blot - Anti-GEF H1 antibody [EPR17963] - C-terminal (ab201687)")
Western blot - Anti-GEF H1 antibody [EPR17963] - C-terminal (ab201687)
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-GEF H1 antibody [EPR17963] - C-terminal (ab201687) at 1/2000 dilution
Lane 1: Mouse brain lysate at 10 µg
Lane 2: Rat brain lysate at 10 µg
Lane 3: Raw264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) lysate at 10 µg
Lane 4: C6 (Rat glial tumor cells) lysate at 10 µg
Lane 5: NIH/3T3 (Mouse embyro fibroblast cells) at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 111 kDa
Observed band size: 112 kDa
Exposure time: 15s
![Immunocytochemistry/ Immunofluorescence - Anti-GEF H1 antibody [EPR17963] - C-terminal (ab201687), expandable thumbnail](https://content.abcam.com/products/images/gef-h1-antibody-epr17963-c-terminal-ab201687--immunocytochemistry-immunofluorescence-img111299.jpg "Immunocytochemistry/ Immunofluorescence - Anti-GEF H1 antibody [EPR17963] - C-terminal (ab201687)")
Immunocytochemistry/ Immunofluorescence - Anti-GEF H1 antibody [EPR17963] - C-terminal (ab201687)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling GEF H1 with ab201687 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/500 dilution (green).
Confocal image showing cytoplasmic staining on HeLa cell line is observed.
The nuclear counter stain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows;
1. ab201687 at 1/500 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
2. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.![Immunocytochemistry/ Immunofluorescence - Anti-GEF H1 antibody [EPR17963] - C-terminal (ab201687), expandable thumbnail](https://content.abcam.com/products/images/gef-h1-antibody-epr17963-c-terminal-ab201687--immunocytochemistry-immunofluorescence-img77422.jpg "Immunocytochemistry/ Immunofluorescence - Anti-GEF H1 antibody [EPR17963] - C-terminal (ab201687)")
Immunocytochemistry/ Immunofluorescence - Anti-GEF H1 antibody [EPR17963] - C-terminal (ab201687)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling GEF H1 with ab201687 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/500 dilution (green).
Confocal image showing cytoplasmic staining on MCF7 cell line is observed.
The nuclear counter stain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows;
1. ab201687 at 1/500 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
2. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.![Flow Cytometry (Intracellular) - Anti-GEF H1 antibody [EPR17963] - C-terminal (ab201687), expandable thumbnail](https://content.abcam.com/products/images/gef-h1-antibody-epr17963-c-terminal-ab201687--flow-cytometry-intracellular-img45872.jpg "Flow Cytometry (Intracellular) - Anti-GEF H1 antibody [EPR17963] - C-terminal (ab201687)")
Flow Cytometry (Intracellular) - Anti-GEF H1 antibody [EPR17963] - C-terminal (ab201687)
Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HEK293 (human embryonic kidney) cells labeling GEF H1 with ab201687 at 1/100 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and a unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
![Immunocytochemistry/ Immunofluorescence - Anti-GEF H1 antibody [EPR17963] - C-terminal (ab201687), expandable thumbnail](https://content.abcam.com/products/images/gef-h1-antibody-epr17963-c-terminal-ab201687--immunocytochemistry-immunofluorescence-img77422.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-GEF H1 antibody [EPR17963] - C-terminal (ab201687)")
![Flow Cytometry (Intracellular) - Anti-GEF H1 antibody [EPR17963] - C-terminal (ab201687), expandable thumbnail](https://content.abcam.com/products/images/gef-h1-antibody-epr17963-c-terminal-ab201687--flow-cytometry-intracellular-img45872.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-GEF H1 antibody [EPR17963] - C-terminal (ab201687)")
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