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AB191134

Anti-Gelsolin antibody - C-terminal

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(1 Publication)

Rabbit Polyclonal GELS antibody. C-terminal. Suitable for IHC-P, ICC, WB and reacts with Rat, Human, Mouse, African green monkey samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human GSN aa 750 to C-terminus.

View Alternative Names

Gelsolin, AGEL, Actin-depolymerizing factor, Brevin, ADF, GSN

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gelsolin antibody - C-terminal (AB191134)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gelsolin antibody - C-terminal (AB191134)

Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue labeling Gelsolin with ab191134 at 1 μg/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gelsolin antibody - C-terminal (AB191134)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gelsolin antibody - C-terminal (AB191134)

Immunohistochemical analysis of paraffin-embedded Human intestinal cancer tissue labeling Gelsolin with ab191134 at 1 μg/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gelsolin antibody - C-terminal (AB191134)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gelsolin antibody - C-terminal (AB191134)

Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labeling Gelsolin with ab191134 at 1 μg/ml.

Immunocytochemistry - Anti-Gelsolin antibody - C-terminal (AB191134)
  • ICC

Supplier Data

Immunocytochemistry - Anti-Gelsolin antibody - C-terminal (AB191134)

Immunocytochemical analysis of NIH3T3 cells labeling Gelsolin with ab191134 at 1 μg/ml.

Western blot - Anti-Gelsolin antibody - C-terminal (AB191134)
  • WB

Supplier Data

Western blot - Anti-Gelsolin antibody - C-terminal (AB191134)

5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.The proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT

All lanes:

Western blot - Anti-Gelsolin antibody - C-terminal (ab191134) at 0.5 µg/mL

Lane 1:

human HepG2 whole cell lysates at 30 µg

Lane 2:

human THP-1 whole cell lysates at 30 µg

Lane 3:

human U251 whole cell lysates at 30 µg

Lane 4:

monkey COS-7 whole cell lysates at 30 µg

Lane 5:

rat C6 whole cell lysates at 30 µg

Lane 6:

mouse NIH/3T3 whole cell lysates at 30 µg

Secondary

All lanes:

goat anti-rabbit IgG-HRP antibody at 1/5000 dilution

true

Western blot - Anti-Gelsolin antibody - C-terminal (AB191134)
  • WB

Supplier Data

Western blot - Anti-Gelsolin antibody - C-terminal (AB191134)

All lanes:

Western blot - Anti-Gelsolin antibody - C-terminal (ab191134) at 0.5 µg/mL

Lane 1:

human HeLa whole cell lysate at 50 µg

Lane 2:

human THP-1 whole cell lysate at 50 µg

Lane 3:

human A549 whole cell lysate at 50 µg

Lane 4:

monkey COS-7 whole cell lysate at 50 µg

Lane 5:

human Caco-2 whole cell lysate at 50 µg

Lane 6:

human K562 whole cell lysate at 50 µg

Lane 7:

human HL-60 whole cell lysate at 50 µg

Secondary

All lanes:

goat anti-rabbit IgG-HRP at 1/10000 dilution

Predicted band size: 86 kDa

false

Western blot - Anti-Gelsolin antibody - C-terminal (AB191134)
  • WB

Supplier Data

Western blot - Anti-Gelsolin antibody - C-terminal (AB191134)

All lanes:

Western blot - Anti-Gelsolin antibody - C-terminal (ab191134) at 0.5 µg/mL

Lane 1:

HeLa cell lysate

Lane 2:

A431 cell lysate

Predicted band size: 86 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human, African green monkey

Applications

IHC-P, ICC, WB

applications

Immunogen

Synthetic Peptide within Human GSN aa 750 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P06396

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.5 µg/mL", "WB-species-notes": "<p>The detection limit for Gelsolin is approximately 1.5ng/lane under reducing conditions.</p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "0.5-1 µg/mL", "ICC-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "African green monkey": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.5 µg/mL", "WB-species-notes": "<p>The detection limit for Gelsolin is approximately 1.5ng/lane under reducing conditions.</p>" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
Preservative: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate) Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Disodium hydrogenorthophosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Gelsolin is a multifunctional actin-binding protein that exists in two main isoforms: cytoplasmic and plasma. It has a molecular mass of approximately 82 kDa. Gelsolin is expressed in a wide range of tissues including muscle epithelial and nerve cells. The protein's main mechanical function involves severing actin filaments which helps to regulate actin polymerization and depolymerization. Researchers sometimes refer to gelsolin as 'GSN'. The presence of the protein helps maintain the dynamic organization of the cytoskeleton by interacting with actin filaments.
Biological function summary

This protein acts in cell movement apoptosis and signal transduction. Gelsolin contributes to the remodeling of the actin cytoskeleton by promoting the disassembly of actin filaments allowing cells to change shape and move. Although it operates independently gelsolin’s activity can be influenced by calcium ions and phosphoinositides leading to its participation in various cellular activities. Gelsolin plays a role in platelet activation and may regulate cellular responses through association with the cytoskeletal complex.

Pathways

Gelsolin participates in the regulation of the actin cytoskeleton and apoptotic pathways. Within these pathways gelsolin interacts with proteins like cofilin to modulate actin filament dynamics. In apoptotic pathways it collaborates by altering the cytoskeletal architecture impacting cell death mechanisms. Gelsolin modifications in these pathways have implications for cellular processes like cell migration and apoptosis bringing it into relation with proteins such as caspases.

Gelsolin is linked to conditions such as gelsolin amyloidosis and cancer metastasis. Gelsolin amyloidosis results from mutations in the gelsolin gene which lead to the formation of amyloid fibrils causing dysfunction in nerves eyes and skin. In cancer altered gelsolin expression can influence tumor progression and metastasis through its role in cell motility. Related proteins such as actin and caspases could also play a part in these conditions either through direct interaction or through pathways in which gelsolin functions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed (PubMed : 19666512). Plays a role in ciliogenesis (PubMed : 20393563).
See full target information GSN
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 22:352 PubMed38622667

2024

Quinic acid regulated TMA/TMAO-related lipid metabolism and vascular endothelial function through gut microbiota to inhibit atherosclerotic.

Applications

Unspecified application

Species

Unspecified reactive species

Qiao Jin,Chiyuan Zhang,Ran Chen,Luping Jiang,Hongli Li,Pengcui Wu,Liang Li
View all publications
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