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Rabbit Recombinant Monoclonal GELS antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 7 publications.

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Images

Western blot - Anti-Gelsolin antibody [EPR1942] (AB109014), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gelsolin antibody [EPR1942] (AB109014), expandable thumbnail
  • Western blot - Anti-Gelsolin antibody [EPR1942] (AB109014), expandable thumbnail
  • Western blot - Anti-Gelsolin antibody [EPR1942] (AB109014), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gelsolin antibody [EPR1942] (AB109014), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Abcam Recommends

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IFFlow Cyt (Intra)
Human
Tested
Not recommended
Tested
Not recommended
Not recommended
Mouse
Predicted
Not recommended
Predicted
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/250 - 1/500
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/20000 - 1/50000
Notes

-

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Species
Mouse
Dilution info
-
Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed (PubMed:19666512). Plays a role in ciliogenesis (PubMed:20393563).

Alternative names

Gelsolin, AGEL, Actin-depolymerizing factor, Brevin, ADF, GSN

Recommended products

Rabbit Recombinant Monoclonal GELS antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 7 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR1942
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Gelsolin is a multifunctional actin-binding protein that exists in two main isoforms: cytoplasmic and plasma. It has a molecular mass of approximately 82 kDa. Gelsolin is expressed in a wide range of tissues including muscle epithelial and nerve cells. The protein's main mechanical function involves severing actin filaments which helps to regulate actin polymerization and depolymerization. Researchers sometimes refer to gelsolin as 'GSN'. The presence of the protein helps maintain the dynamic organization of the cytoskeleton by interacting with actin filaments.

Biological function summary

This protein acts in cell movement apoptosis and signal transduction. Gelsolin contributes to the remodeling of the actin cytoskeleton by promoting the disassembly of actin filaments allowing cells to change shape and move. Although it operates independently gelsolin’s activity can be influenced by calcium ions and phosphoinositides leading to its participation in various cellular activities. Gelsolin plays a role in platelet activation and may regulate cellular responses through association with the cytoskeletal complex.

Pathways

Gelsolin participates in the regulation of the actin cytoskeleton and apoptotic pathways. Within these pathways gelsolin interacts with proteins like cofilin to modulate actin filament dynamics. In apoptotic pathways it collaborates by altering the cytoskeletal architecture impacting cell death mechanisms. Gelsolin modifications in these pathways have implications for cellular processes like cell migration and apoptosis bringing it into relation with proteins such as caspases.

Associated diseases and disorders

Gelsolin is linked to conditions such as gelsolin amyloidosis and cancer metastasis. Gelsolin amyloidosis results from mutations in the gelsolin gene which lead to the formation of amyloid fibrils causing dysfunction in nerves eyes and skin. In cancer altered gelsolin expression can influence tumor progression and metastasis through its role in cell motility. Related proteins such as actin and caspases could also play a part in these conditions either through direct interaction or through pathways in which gelsolin functions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-Gelsolin antibody [EPR1942] (ab109014), expandable thumbnail

    Western blot - Anti-Gelsolin antibody [EPR1942] (ab109014)

    ab109014 was shown to react with GSN in wild-type HeLa cells in Western blot with loss of signal observed in GSN knockout cell line Human GSN (Gelsolin) knockout HeLa cell line ab265201 (GSN knockout cell lysate Human GSN (Gelsolin) knockout HeLa cell lysate ab257204). Wild-type HeLa and GSN knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab109014 overnight at 4 °C at a 1/20000 dilution. Blots were incubated with goat anti-rabbit HRP secondary antibodies at 1/5000 before imaging. These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

    All lanes: Western blot - Anti-Gelsolin antibody [EPR1942] (ab109014) at 1/20000 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: GSN knockout HeLa cell lysate at 20 µg

    Lane 2: Western blot - Human GSN (Gelsolin) knockout HeLa cell line (Human GSN (Gelsolin) knockout HeLa cell line ab265201)

    Performed under reducing conditions.

    Predicted band size: 86 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gelsolin antibody [EPR1942] (ab109014), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gelsolin antibody [EPR1942] (ab109014)

    Immunohistochemical analysis of Gelsolin expression in paraffin-embedded human kidney tissue using ab109014 at 1/250 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-Gelsolin antibody [EPR1942] (ab109014), expandable thumbnail

    Western blot - Anti-Gelsolin antibody [EPR1942] (ab109014)

    Blocking buffer 5% NFDM/TBST
    Diluting buffer 5% NFDM/TBST

    The secretory isoform of gelsolin is at 86kDa. The cytoplasmic isoforms of gelsolin are at 81kDa.

    All lanes: Western blot - Anti-Gelsolin antibody [EPR1942] (ab109014) at 1/20000 dilution

    Lane 1: THP-1 whole cell lysate at 10 µg

    Lane 2: THP-1 cultured medium at 10 µg

    Lane 3: MCF-7 whole cell lysate at 10 µg

    Lane 4: Human Plasma at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 86 kDa

    Observed band size: 81 kDa, 86 kDa

    Exposure time: 10s

  • Western blot - Anti-Gelsolin antibody [EPR1942] (ab109014), expandable thumbnail

    Western blot - Anti-Gelsolin antibody [EPR1942] (ab109014)

    All lanes: Western blot - Anti-Gelsolin antibody [EPR1942] (ab109014) at 1/50000 dilution

    Lane 1: MCF7 cell lysate at 10 µg

    Lane 2: HL60 cell lysate at 10 µg

    Lane 3: A431 cell lysate at 10 µg

    Lane 4: HeLa cell lysate at 10 µg

    Predicted band size: 86 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gelsolin antibody [EPR1942] (ab109014), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gelsolin antibody [EPR1942] (ab109014)

    Immunohistochemical analysis of Gelsolin expression in paraffin-embedded Human lung tissue using ab109014 at 1/250 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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