Anti-Gelsolin antibody [EPR1942] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gelsolin antibody [EPR1942] - BSA and Azide free (AB236029)

Immunohistochemical analysis of Gelsolin expression in paraffin-embedded human kidney tissue using ab109014 at 1/250 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109014).

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gelsolin antibody [EPR1942] - BSA and Azide free (AB236029)

Immunohistochemical analysis of Gelsolin expression in paraffin-embedded Human lung tissue using ab109014 at 1/250 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109014).

• WB

Collaborator

Western blot - Anti-Gelsolin antibody [EPR1942] - BSA and Azide free (AB236029)

This data was developed using the same antibody in a different buffer formulation (ab109014).

ab109014 was shown to react with GSN in wild-type HeLa cells in Western blot with loss of signal observed in GSN knockout cell line ab265201 (GSN knockout cell lysate ab257204). Wild-type HeLa and GSN knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab109014 overnight at 4 °C at a 1/20000 dilution. Blots were incubated with goat anti-rabbit HRP secondary antibodies at 1/5000 before imaging. These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Western blot - Anti-Gelsolin antibody [EPR1942] (<a href='/en-us/products/primary-antibodies/gelsolin-antibody-epr1942-ab109014'>ab109014</a>) at 1/20000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

GSN knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human GSN (Gelsolin) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-gsn-gelsolin-knockout-hela-cell-line-ab265201'>ab265201</a>)

Predicted band size: 86 kDa

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