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anti-Gelsolin plasma antibody [EPR1941Y] ab247406 is a carrier free rabbit monoclonal antibody that is used in Gelsolin plasma western blotting, IHC and Immunoprecipitation. Suitable for human and mouse samples.

- PBS only, conjugation-ready, removing anything extra from your antibodies, greater flexibility in assay design
- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray validation

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Images

Western blot - Anti-Gelsolin plasma antibody [EPR1941Y] - BSA and Azide free (AB247406), expandable thumbnail
  • Immunoprecipitation - Anti-Gelsolin plasma antibody [EPR1941Y] - BSA and Azide free (AB247406), expandable thumbnail
  • Western blot - Anti-Gelsolin plasma antibody [EPR1941Y] - BSA and Azide free (AB247406), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gelsolin plasma antibody [EPR1941Y] - BSA and Azide free (AB247406), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytIHC-PICC/IFIPWB
Human
Not recommended
Tested
Not recommended
Expected
Tested
Mouse
Not recommended
Expected
Not recommended
Tested
Expected

Not recommended
Not recommended

Species
Mouse, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed (PubMed:19666512). Plays a role in ciliogenesis (PubMed:20393563).

Alternative names

Gelsolin, AGEL, Actin-depolymerizing factor, Brevin, ADF, GSN

Recommended products

anti-Gelsolin plasma antibody [EPR1941Y] ab247406 is a carrier free rabbit monoclonal antibody that is used in Gelsolin plasma western blotting, IHC and Immunoprecipitation. Suitable for human and mouse samples.

- PBS only, conjugation-ready, removing anything extra from your antibodies, greater flexibility in assay design
- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray validation

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR1941Y
Purification technique
Affinity purification Protein A
Specificity

The antibody only detects the isoform 1 of the protein (86kDa).

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab247406 is the carrier-free version of Anti-Gelsolin plasma antibody [EPR1941Y] ab75832.

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Gelsolin plasma also known as plasma gelsolin is a multifunctional actin-binding protein. It has a molecular mass of approximately 86 kDa. Gelsolin plasma severs and caps actin filaments playing a critical role in the reorganization of the actin cytoskeleton. It is expressed in the cytoplasm and secreted into the blood plasma facilitating actin filament turnover and cellular motility. It also binds to phosphatidylinositol 45-bisphosphate (PIP2) which affects its activity and regulation.

Biological function summary

Gelsolin plasma significantly impacts cell shape movement and signaling. It dismantles actin polymers converting them into monomeric actin important for maintaining the dynamic equilibrium of actin filaments. Gelsolin plasma also participates in the immune response and inflammation modulation. While not specifically part of a larger protein complex it interacts with other cytoskeletal elements and signaling molecules.

Pathways

The functions of gelsolin plasma integrate primarily in the actin cytoskeleton regulation pathway and PIP2 signaling pathway. In the actin cytoskeleton pathway it works alongside proteins such as cofilin and profilin to mediate actin dynamics. In the PIP2 signaling pathway gelsolin plasma interacts with PIP2 which influences its ability to modulate actin filament assembly and disassembly. These pathways are essential for processes like cell migration and cellular signaling.

Associated diseases and disorders

Gelsolin plasma shows involvement in conditions such as familial amyloidosis and inflammation-related diseases. Mutations in gelsolin can lead to familial amyloidosis of the Finnish type characterized by gelsolin-derived amyloid deposits. Moreover alterations in gelsolin levels relate to inflammatory disorders affecting the body's response to injury and infection. The protein also links to other amyloidogenic proteins emphasizing its role in amyloidosis pathology.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com