AB150383

Anti-Gemin 2 antibody [EPR10040(B)]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal Gemin 2 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

View Alternative Names

SIP1, GEMIN2, Gem-associated protein 2, Gemin-2, Component of gems 2, Survival of motor neuron protein-interacting protein 1, SMN-interacting protein 1

1 Images

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Western blot - Anti-Gemin 2 antibody [EPR10040(B)] (AB150383)

All lanes:

Western blot - Anti-Gemin 2 antibody [EPR10040(B)] (ab150383) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 31 kDa

false

Carrier free

Anti-Gemin 2 antibody [EPR10040(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10040(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Gemin 2 also known simply as "gemin" is a protein identified by its mass of approximately 29 kDa. The protein is an essential component in the survival of motor neuron (SMN) complex. Gemin 2 localizes mainly in the cytoplasm and is expressed in a variety of tissues throughout the body with a notable presence in motor neurons. This protein works closely within a multi-protein assembly regulating several functions related to the synthesis and processing of RNA.

Biological function summary

Gemin 2 plays a role in the assembly of small nuclear ribonucleoproteins (snRNPs) which are integral to pre-mRNA splicing. As part of the SMN complex Gemin 2 ensures the proper assembly and delivery of snRNPs to the spliceosome. This activity is necessary for accurate splicing of pre-mRNA which is a critical step in gene expression. The protein's binding and interaction capabilities stabilize this complex contributing to efficient splicing processes.

Pathways

Gemin 2 is integral to RNA processing mechanisms and the RNP biogenesis pathway. It associates with other proteins like Gemin 6 and Gemin 7 within the SMN complex to promote snRNP biogenesis and function. Additionally Gemin 2 contributes to pathways that oversee proper mRNA processing and stability ensuring that gene expression is accurately regulated in the cell.

Mutations or dysfunctions in the Gemin 2 protein are linked with spinal muscular atrophy (SMA) a neurodegenerative disorder affecting motor neurons. In SMA the SMN complex including Gemin 2 is disrupted leading to deficits in snRNP assembly and widespread splicing defects. Also disruptions in this protein may have connections to muscular dystrophies where imbalances in splicing can cause muscle weakness and atrophy. Gemin 2's interplay with the SMN protein is particularly significant in these disorders as their interaction is critical for maintaining motor neuron function and viability.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs (PubMed : 18984161, PubMed : 9323129). Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP (Sm core) (PubMed : 18984161). In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG (5Sm) are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP (PubMed : 18984161). To assemble core snRNPs, the SMN complex accepts the trapped 5Sm proteins from CLNS1A (PubMed : 18984161, PubMed : 9323129). Binding of snRNA inside 5Sm ultimately triggers eviction of the SMN complex, thereby allowing binding of SNRPD3 and SNRPB to complete assembly of the core snRNP (PubMed : 31799625). Within the SMN complex, GEMIN2 constrains the conformation of 5Sm, thereby promoting 5Sm binding to snRNA containing the snRNP code (a nonameric Sm site and a 3'-adjacent stem-loop), thus preventing progression of assembly until a cognate substrate is bound (PubMed : 16314521, PubMed : 21816274, PubMed : 31799625).

See full target information GEMIN2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell reports. Medicine 5:101659 PubMed39067446

2024

Isogenic patient-derived organoids reveal early neurodevelopmental defects in spinal muscular atrophy initiation.

Applications

Unspecified application

Species

Unspecified reactive species

Tobias Grass,Zeynep Dokuzluoglu,Felix Buchner,Ines Rosignol,Joshua Thomas,Antonio Caldarelli,Anna Dalinskaya,Jutta Becker,Fabian Rost,Michele Marass,Brunhilde Wirth,Marc Beyer,Lorenzo Bonaguro,Natalia Rodriguez-Muela

The Journal of clinical investigation 128:3008-3023 PubMed29672276

2018

Blocking p62-dependent SMN degradation ameliorates spinal muscular atrophy disease phenotypes.

Applications

Unspecified application

Species

Unspecified reactive species

Natalia Rodriguez-Muela,Andrey Parkhitko,Tobias Grass,Rebecca M Gibbs,Erika M Norabuena,Norbert Perrimon,Rajat Singh,Lee L Rubin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Gemin 2 antibody [EPR10040(B)]

Western blot - Anti-Gemin 2 antibody [EPR10040(B)] (AB150383)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise