Rabbit Recombinant Monoclonal Gemin 2 antibody. Carrier free. Suitable for WB and reacts with Human samples.
pH: 7.2 - 7.4
Constituents: PBS
ICC/IF | Flow Cyt | WB | IHC-P | |
---|---|---|---|---|
Human | Not recommended | Not recommended | Tested | Not recommended |
Mouse | Not recommended | Not recommended | Predicted | Not recommended |
Rat | Not recommended | Not recommended | Predicted | Not recommended |
Species | Dilution info | Notes |
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Species Human, Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Human, Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Human, Mouse, Rat | Dilution info - | Notes - |
The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs (PubMed:18984161, PubMed:9323129). Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP (Sm core) (PubMed:18984161). In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG (5Sm) are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP (PubMed:18984161). To assemble core snRNPs, the SMN complex accepts the trapped 5Sm proteins from CLNS1A (PubMed:18984161, PubMed:9323129). Binding of snRNA inside 5Sm ultimately triggers eviction of the SMN complex, thereby allowing binding of SNRPD3 and SNRPB to complete assembly of the core snRNP (PubMed:31799625). Within the SMN complex, GEMIN2 constrains the conformation of 5Sm, thereby promoting 5Sm binding to snRNA containing the snRNP code (a nonameric Sm site and a 3'-adjacent stem-loop), thus preventing progression of assembly until a cognate substrate is bound (PubMed:16314521, PubMed:21816274, PubMed:31799625).
SIP1, GEMIN2, Gem-associated protein 2, Gemin-2, Component of gems 2, Survival of motor neuron protein-interacting protein 1, SMN-interacting protein 1
Rabbit Recombinant Monoclonal Gemin 2 antibody. Carrier free. Suitable for WB and reacts with Human samples.
pH: 7.2 - 7.4
Constituents: PBS
ab248955 is the carrier-free version of Anti-Gemin 2 antibody [EPR10040(B)] ab150383.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Gemin 2 also known simply as "gemin" is a protein identified by its mass of approximately 29 kDa. The protein is an essential component in the survival of motor neuron (SMN) complex. Gemin 2 localizes mainly in the cytoplasm and is expressed in a variety of tissues throughout the body with a notable presence in motor neurons. This protein works closely within a multi-protein assembly regulating several functions related to the synthesis and processing of RNA.
Gemin 2 plays a role in the assembly of small nuclear ribonucleoproteins (snRNPs) which are integral to pre-mRNA splicing. As part of the SMN complex Gemin 2 ensures the proper assembly and delivery of snRNPs to the spliceosome. This activity is necessary for accurate splicing of pre-mRNA which is a critical step in gene expression. The protein's binding and interaction capabilities stabilize this complex contributing to efficient splicing processes.
Gemin 2 is integral to RNA processing mechanisms and the RNP biogenesis pathway. It associates with other proteins like Gemin 6 and Gemin 7 within the SMN complex to promote snRNP biogenesis and function. Additionally Gemin 2 contributes to pathways that oversee proper mRNA processing and stability ensuring that gene expression is accurately regulated in the cell.
Mutations or dysfunctions in the Gemin 2 protein are linked with spinal muscular atrophy (SMA) a neurodegenerative disorder affecting motor neurons. In SMA the SMN complex including Gemin 2 is disrupted leading to deficits in snRNP assembly and widespread splicing defects. Also disruptions in this protein may have connections to muscular dystrophies where imbalances in splicing can cause muscle weakness and atrophy. Gemin 2's interplay with the SMN protein is particularly significant in these disorders as their interaction is critical for maintaining motor neuron function and viability.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Terms & Conditions.
This data was developed using Anti-Gemin 2 antibody [EPR10040(B)] ab150383, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Gemin 2 antibody [EPR10040(B)] - BSA and Azide free (ab248955) at 1/1000 dilution
Lane 1: HepG2 cell lysate at 10 µg
Lane 2: K562 cell lysate at 10 µg
Lane 3: HeLa cell lysate at 10 µg
All lanes: HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 31 kDa
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