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Rabbit Recombinant Monoclonal Gemin 2 antibody. Carrier free. Suitable for WB and reacts with Human samples.

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Images

Western blot - Anti-Gemin 2 antibody [EPR10040(B)] - BSA and Azide free (AB248955), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFFlow CytWBIHC-P
Human
Not recommended
Not recommended
Tested
Not recommended
Mouse
Not recommended
Not recommended
Predicted
Not recommended
Rat
Not recommended
Not recommended
Predicted
Not recommended

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs (PubMed:18984161, PubMed:9323129). Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP (Sm core) (PubMed:18984161). In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG (5Sm) are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP (PubMed:18984161). To assemble core snRNPs, the SMN complex accepts the trapped 5Sm proteins from CLNS1A (PubMed:18984161, PubMed:9323129). Binding of snRNA inside 5Sm ultimately triggers eviction of the SMN complex, thereby allowing binding of SNRPD3 and SNRPB to complete assembly of the core snRNP (PubMed:31799625). Within the SMN complex, GEMIN2 constrains the conformation of 5Sm, thereby promoting 5Sm binding to snRNA containing the snRNP code (a nonameric Sm site and a 3'-adjacent stem-loop), thus preventing progression of assembly until a cognate substrate is bound (PubMed:16314521, PubMed:21816274, PubMed:31799625).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal Gemin 2 antibody. Carrier free. Suitable for WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR10040(B)
Purification technique
Affinity purification
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab248955 is the carrier-free version of Anti-Gemin 2 antibody [EPR10040(B)] ab150383.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Gemin 2 also known simply as "gemin" is a protein identified by its mass of approximately 29 kDa. The protein is an essential component in the survival of motor neuron (SMN) complex. Gemin 2 localizes mainly in the cytoplasm and is expressed in a variety of tissues throughout the body with a notable presence in motor neurons. This protein works closely within a multi-protein assembly regulating several functions related to the synthesis and processing of RNA.

Biological function summary

Gemin 2 plays a role in the assembly of small nuclear ribonucleoproteins (snRNPs) which are integral to pre-mRNA splicing. As part of the SMN complex Gemin 2 ensures the proper assembly and delivery of snRNPs to the spliceosome. This activity is necessary for accurate splicing of pre-mRNA which is a critical step in gene expression. The protein's binding and interaction capabilities stabilize this complex contributing to efficient splicing processes.

Pathways

Gemin 2 is integral to RNA processing mechanisms and the RNP biogenesis pathway. It associates with other proteins like Gemin 6 and Gemin 7 within the SMN complex to promote snRNP biogenesis and function. Additionally Gemin 2 contributes to pathways that oversee proper mRNA processing and stability ensuring that gene expression is accurately regulated in the cell.

Associated diseases and disorders

Mutations or dysfunctions in the Gemin 2 protein are linked with spinal muscular atrophy (SMA) a neurodegenerative disorder affecting motor neurons. In SMA the SMN complex including Gemin 2 is disrupted leading to deficits in snRNP assembly and widespread splicing defects. Also disruptions in this protein may have connections to muscular dystrophies where imbalances in splicing can cause muscle weakness and atrophy. Gemin 2's interplay with the SMN protein is particularly significant in these disorders as their interaction is critical for maintaining motor neuron function and viability.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Western blot - Anti-Gemin 2 antibody [EPR10040(B)] - BSA and Azide free (ab248955), expandable thumbnail

    Western blot - Anti-Gemin 2 antibody [EPR10040(B)] - BSA and Azide free (ab248955)

    This data was developed using Anti-Gemin 2 antibody [EPR10040(B)] ab150383, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-Gemin 2 antibody [EPR10040(B)] - BSA and Azide free (ab248955) at 1/1000 dilution

    Lane 1: HepG2 cell lysate at 10 µg

    Lane 2: K562 cell lysate at 10 µg

    Lane 3: HeLa cell lysate at 10 µg

    Secondary

    All lanes: HRP labeled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 31 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com